Abstract
• A set of 11, 9 and 24 AIF genes were identified in B. rapa, B. oleracea , and B. napus , respectively. • BnaAIF1-C03 is highly induced under low-temperature. • Overexpression of BnaAIF1-C03 significantly improved the seed germination under cold stress (4 °C) and the survival rate of seedlings under freezing stress (−4 °C) in rapeseed. • BnaAIF1-C03, localized in the nucleus, interacts with BnaICE1 to activate the ICE1-CBF-COR pathway, thereby enhancing freezing tolerance. Rapeseed ( Brassica napus L.) is an important oilseed crop in the world, it’s often damaged by low-temperature (especially freezing) stress, which has the potential to cause significant yield losses. However, little is known about the molecular mechanisms for coping with low-temperature stress in rapeseed. In this study, a total of 24 atypical bHLH transcription factor AIF (ATBS1 INTERACTING FACTOR) gene family members were identified by systematically bioinformatics analysis in rapeseed, among which all six BnaAIF1 genes were strongly induced by low temperature stress, especially BnaAIF1-A03/C03 . Overexpression of BnaAIF1-C03 improved the seed germination under cold stress (4 °C) and the survival rate of seedlings under freezing stress (-4 °C) in rapeseed, with enhanced stability of light system II (Fv/Fm and NPQ_Lss increased) and reduced oxidative damage (decreased contents of H 2 O 2 and MDA). Further analysis revealed that BnaAIF1-C03 was localized in the nucleus and interacted with BnaICE1, activating the ICE1-CBF-COR pathway genes and thus participating in the low-temperature stress response of rapeseed. Overall, this study provides an important theoretical basis and new target gene for future breeding of low-temperature tolerant rapeseed.
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Claims 11
Claim · model-written summary
Eleven AIF genes were identified in B. rapa.
- Taxon
- B. rapa
Brassica rapae0 - Gene
- AIF genese0
- Outcome
- AIF genes were identifiede0
- Value
- 11e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“11, 9 and 24 AIF genes were identified in B. rapa, B. oleracea , and B. napus , respectively”
Claim · model-written summary
Nine AIF genes were identified in B. oleracea.
- Taxon
- B. oleracea
Brassica oleraceae0 - Gene
- AIF genese0
- Outcome
- AIF genes were identifiede0
- Value
- 9e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“11, 9 and 24 AIF genes were identified in B. rapa, B. oleracea , and B. napus , respectively”
Claim · model-written summary
Twenty-four AIF genes were identified in B. napus.
- Taxon
- B. napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napuse0 - Gene
- AIF genese0
- Outcome
- AIF genes were identifiede0
- Value
- 24e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“11, 9 and 24 AIF genes were identified in B. rapa, B. oleracea , and B. napus , respectively”
Claim · model-written summary
All six BnaAIF1 genes were strongly induced by low temperature, especially BnaAIF1-A03/C03.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- low temperature stresse0
- Gene
- BnaAIF1 genese0
- Outcome
- BnaAIF1 genese0
- Direction
- strongly inducede0
- Value
- sixe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all six BnaAIF1 genes were strongly induced by low temperature stress, especially BnaAIF1-A03/C03”
t0“Brassica napus”
Claim · model-written summary
BnaAIF1-C03 overexpression improved seed germination at 4 °C.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- Overexpression of BnaAIF1-C03e0
- Gene
- BnaAIF1-C03e0
- Outcome
- seed germinatione0
- Direction
- improvede0
- Qualifier
- cold stress (4 °C)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of BnaAIF1-C03 improved the seed germination under cold stress (4 °C)”
t0“Brassica napus”
Claim · model-written summary
BnaAIF1-C03 overexpression improved seedling survival at −4 °C.
- Taxon
- rapeseede0
- Treatment
- Overexpression of BnaAIF1-C03e0
- Gene
- BnaAIF1-C03e0
- Outcome
- survival rate of seedlingse0
- Direction
- improvede0
- Qualifier
- freezing stress (-4 °C)e0
Not reportedComparator
Verbatim
e0“Overexpression of BnaAIF1-C03 improved the seed germination under cold stress (4 °C) and the survival rate of seedlings under freezing stress (-4 °C) in rapeseed”
Claim · model-written summary
Overexpression increased Fv/Fm and NPQ_Lss, indicating more stable photosystem II.
Not reportedComparator
Verbatim
e0“Overexpression of BnaAIF1-C03 improved the seed germination under cold stress (4 °C) and the survival rate of seedlings under freezing stress (-4 °C) in rapeseed, with enhanced stability of light system II (Fv/Fm and NPQ_Lss increased)”
Claim · model-written summary
Overexpression decreased hydrogen peroxide and MDA contents.
Not reportedComparator
Verbatim
e0“Overexpression of BnaAIF1-C03 improved the seed germination under cold stress (4 °C) and the survival rate of seedlings under freezing stress (-4 °C) in rapeseed, with enhanced stability of light system II (Fv/Fm and NPQ_Lss increased) and reduced oxidative damage (decreased contents of H 2 O 2 and MDA)”
Claim · model-written summary
BnaAIF1-C03 was localized in the nucleus.
- Taxon
- Brassica napusBrassica napust0
- Gene
- BnaAIF1-C03e0
- Outcome
- localized in the nucleuse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BnaAIF1-C03 was localized in the nucleus”
t0“Brassica napus”
Claim · model-written summary
BnaAIF1-C03 interacted with BnaICE1.
- Taxon
- Brassica napusBrassica napust0
- Gene
- BnaAIF1-C03e0
- Outcome
- interacted with BnaICE1e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BnaAIF1-C03 was localized in the nucleus and interacted with BnaICE1”
t0“Brassica napus”
Claim · model-written summary
The BnaAIF1-C03–BnaICE1 interaction activated ICE1-CBF-COR pathway genes in the low-temperature response.
Verbatim
e0“interacted with BnaICE1, activating the ICE1-CBF-COR pathway genes and thus participating in the low-temperature stress response of rapeseed”
The same questions in other papers
cold · molecular: expression · Brassica napus (oilseed rape)Positive and negative results
12 papers in the same direction as this paper · 8 in the opposite direction
- no effect
- no effect
- no effect
17 papers more in this group
See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.