Paper · 2025 · Baghdad Journal of Biochemistry and Applied Biological Sciences

Assessing the Effects of Blanching and Pickling on the Microbiological Quality and Nutritional Retention of Cabbage

Nnenna Omorodion; Adikea Ezinne H.

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citations
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Open access · cc-by

Abstract

Background: Cabbage is a widely consumed vegetable that is highly perishable and prone tomicrobial contamination. Processing techniques such as blanching and pickling can improveits safety and extend shelf life, but their effects on microbial quality and nutrient compositionrequire systematic evaluation.Objective: This study investigated the impact of blanching at varying time–temperature conditions and pickling on the microbial safety and nutritional composition of cabbage.Materials and Methods: Fresh cabbage samples were blanched at 80°C for 1 min, 85°C for 2 min,90°C for 3 min, 95°C for 5 min, and 100°C for 7 min, or processed by pickling. A control samplewas left untreated. Microbial quality was assessed using standard microbiological techniques,including enumeration of total heterotrophic bacterial counts (THBC), staphylococci (TSC),fungi (TFC), and coliforms. Proximate composition (moisture, ash, protein, fiber, and carbohydrate) was determined using standard analytical methods.Results: THBC progressively declined with increasing blanching temperature and duration,with the lowest counts observed at 95–100°C, where bacterial growth was nearly inhibitedafter week 3. Pickled samples showed a steady decline (4.6 × 10³ to 1.2 × 10³ CFU/g), whilecontrols retained high counts (>10⁵ CFU/g). TSC was effectively inhibited from week 2 at ≥90°C,whereas pickling reduced counts by week 2. Fungal growth declined substantially at 90–95°C(as low as 4.4 × 10² CFU/g), while coliforms were absent in all treated samples. Nutritionalanalysis indicated that blanching enhanced crude protein and fiber retention, while picklingincreased moisture but reduced ash content. Carbohydrates remained relatively constantacross treatments.Conclusion: Blanching, particularly at ≥90°C, and pickling significantly improved microbialsafety of cabbage while maintaining key nutrients. These findings underscore the potential ofboth techniques as practical preservation methods for producing safe, nutritious, and shelf-stable cabbage products.

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Claims 11

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Bacterial counts declined as blanching temperature and duration increased.

Treatment
blanchinge0
Outcome
THBCe0
Direction
declinede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. capitata
Brassica oleracea var. capitata · TaxonThamizhpparithi Maari · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“THBC progressively declined with increasing blanching temperature and duration”

✓ located verbatim in the source · abstract, characters 1008–1086 · show in text ↑
t0

“Cabbage”

✓ located verbatim in the source · title, characters 108–115 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

At 95–100°C bacterial growth was nearly inhibited after week 3.

Treatment
95–100°Ce0
Outcome
bacterial growthe0
Direction
nearly inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. capitata
Brassica oleracea var. capitata · TaxonThamizhpparithi Maari · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“lowest counts observed at 95–100°C, where bacterial growth was nearly inhibitedafter week 3”

✓ located verbatim in the source · abstract, characters 1096–1187 · show in text ↑
t0

“Cabbage”

✓ located verbatim in the source · title, characters 108–115 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Pickling reduced bacterial counts from 4.6 × 10³ to 1.2 × 10³ CFU/g, while control counts remained above 10⁵ CFU/g.

Treatment
Picklede0
Outcome
countse0
Direction
declinee0
Value
4.6 × 10³ to 1.2 × 10³ CFU/ge0
Comparator
controlse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. capitata
Brassica oleracea var. capitata · TaxonThamizhpparithi Maari · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Pickled samples showed a steady decline (4.6 × 10³ to 1.2 × 10³ CFU/g), whilecontrols retained high counts (>10⁵ CFU/g)”

✓ located verbatim in the source · abstract, characters 1189–1308 · show in text ↑
t0

“Cabbage”

✓ located verbatim in the source · title, characters 108–115 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Staphylococci were inhibited from week 2 by blanching at ≥90°C.

Treatment
≥90°Ce0
Outcome
TSCe0
Direction
inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. capitata
Brassica oleracea var. capitata · TaxonThamizhpparithi Maari · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“TSC was effectively inhibited from week 2 at ≥90°C”

✓ located verbatim in the source · abstract, characters 1310–1360 · show in text ↑
t0

“Cabbage”

✓ located verbatim in the source · title, characters 108–115 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Pickling reduced staphylococci counts by week 2.

Treatment
picklinge0
Outcome
countse0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. capitata
Brassica oleracea var. capitata · TaxonThamizhpparithi Maari · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“pickling reduced counts by week 2”

✓ located verbatim in the source · abstract, characters 1369–1402 · show in text ↑
e1

“TSC was effectively inhibited from week 2”

✓ located verbatim in the source · abstract, characters 1310–1351 · show in text ↑
t0

“Cabbage”

✓ located verbatim in the source · title, characters 108–115 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Fungal growth declined substantially at 90–95°C, reaching 4.4 × 10² CFU/g.

Treatment
90–95°Ce0
Outcome
Fungal growthe0
Direction
declinede0
Value
4.4 × 10² CFU/ge0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. capitata
Brassica oleracea var. capitata · TaxonThamizhpparithi Maari · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Fungal growth declined substantially at 90–95°C(as low as 4.4 × 10² CFU/g)”

✓ located verbatim in the source · abstract, characters 1404–1478 · show in text ↑
t0

“Cabbage”

✓ located verbatim in the source · title, characters 108–115 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Coliforms were absent from all treated cabbage samples.

Outcome
coliformse0
Direction
absente0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. capitata
Brassica oleracea var. capitata · TaxonThamizhpparithi Maari · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“coliforms were absent in all treated samples”

✓ located verbatim in the source · abstract, characters 1486–1530 · show in text ↑
t0

“Cabbage”

✓ located verbatim in the source · title, characters 108–115 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Blanching improved crude protein and fiber retention.

Treatment
blanchinge0
Outcome
crude protein and fiber retentione0
Direction
enhancede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. capitata
Brassica oleracea var. capitata · TaxonThamizhpparithi Maari · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“blanching enhanced crude protein and fiber retention”

✓ located verbatim in the source · abstract, characters 1567–1619 · show in text ↑
t0

“Cabbage”

✓ located verbatim in the source · title, characters 108–115 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Pickling increased cabbage moisture.

Treatment
picklinge0
Outcome
moisturee0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. capitata
Brassica oleracea var. capitata · TaxonThamizhpparithi Maari · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“picklingincreased moisture but reduced ash content”

✓ located verbatim in the source · abstract, characters 1627–1677 · show in text ↑
t0

“Cabbage”

✓ located verbatim in the source · title, characters 108–115 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Pickling reduced cabbage ash content.

Treatment
picklinge0
Outcome
ash contente0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. capitata
Brassica oleracea var. capitata · TaxonThamizhpparithi Maari · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“picklingincreased moisture but reduced ash content”

✓ located verbatim in the source · abstract, characters 1627–1677 · show in text ↑
t0

“Cabbage”

✓ located verbatim in the source · title, characters 108–115 · show in text ↑
Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

Carbohydrate content remained relatively constant across treatments.

Outcome
Carbohydratese0
Direction
relatively constante0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. capitata
Brassica oleracea var. capitata · TaxonThamizhpparithi Maari · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Carbohydrates remained relatively constantacross treatments”

✓ located verbatim in the source · abstract, characters 1679–1738 · show in text ↑
t0

“Cabbage”

✓ located verbatim in the source · title, characters 108–115 · show in text ↑

processing · soil: biology · Brassica oleracea (wild cabbage)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

processing · quality: protein · Brassica oleracea (wild cabbage)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.