Paper · 2026 · Molecules

Germination as a Sustainable Green Pre-Treatment for the Recovery and Enhancement of High-Value Compounds in Broccoli and Kale

Christine (Neagu) Dragomir; Corina Dana Misca; Sylvestre Dossa; et al.

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Open access · cc-by

Abstract

Germination is widely recognized as an effective strategy to enhance the nutritional quality and reduce anti-nutritional factors in plant foods. This study evaluated the impact of germination on Cruciferous vegetables (family Cruciferae or Brassicaceae) broccoli and kale by assessing changes in proximate composition, macro- and microelement profiles, total and individual polyphenols, phytic acid content, antimicrobial activity, and structural characteristics using Fourier Transform Infrared Spectroscopy (FTIR) and Small- and Wide-Angle X-ray Scattering (SAXS/WAXS) analyses. Germination significantly increased protein content (30.33% in broccoli sprouts and 30.21% in kale sprouts), total phenolic content (424.40 mg/100 g in broccoli sprouts and 497.94 mg/100 g in kale sprouts), and essential minerals, while reducing phytic acid levels in both species (up to 82.20%). Antimicrobial effects were matrix-dependent, being detected in broccoli and kale seed powders, while no inhibitory activity was observed for the corresponding sprout powders under the tested conditions. FTIR spectra indicated notable modifications in functional groups related to carbohydrates, proteins, and phenolic compounds, while SAXS analysis revealed structural reorganizations at the nanoscale. Overall, germination improved the nutritional and phytochemical quality of broccoli and kale while decreasing anti-nutritional compounds, highlighting its potential to enhance the health-promoting value of Brassica sprouts.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 10

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Germination significantly increased broccoli sprout protein content to 30.33%.

Treatment
Germinatione0
Outcome
protein contente0
Direction
increasede0
Value
30.33%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Germination significantly increased protein content (30.33% in broccoli sprouts and 30.21% in kale sprouts)”

✓ located verbatim in the source · abstract, characters 581–688 · show in text ↑
t0

“Broccoli”

✓ located verbatim in the source · title, characters 109–117 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Germination significantly increased kale sprout protein content to 30.21%.

Treatment
Germinatione0
Outcome
protein contente0
Direction
increasede0
Value
30.21%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. viridis
Brassica oleracea var. viridis · TaxonAnn Perry · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Germination significantly increased protein content (30.33% in broccoli sprouts and 30.21% in kale sprouts)”

✓ located verbatim in the source · abstract, characters 581–688 · show in text ↑
t0

“Kale”

✓ located verbatim in the source · title, characters 122–126 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Germination significantly increased broccoli sprout total phenolics to 424.40 mg/100 g.

Treatment
Germinationt0
Outcome
total phenolic contente0
Value
424.40 mg/100 ge0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“total phenolic content (424.40 mg/100 g in broccoli sprouts and 497.94 mg/100 g in kale sprouts)”

✓ located verbatim in the source · abstract, characters 690–786 · show in text ↑
t0

“Germination as a Sustainable Green Pre-Treatment for the Recovery and Enhancement of High-Value Compounds in Broccoli and Kale”

✓ located verbatim in the source · title, characters 0–126 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Germination significantly increased kale sprout total phenolics to 497.94 mg/100 g.

Treatment
Germinationt0
Outcome
total phenolic contente0
Value
497.94 mg/100 ge0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. viridis
Brassica oleracea var. viridis · TaxonAnn Perry · Public domain · Wikimedia Commons ↗

Verbatim

e0

“total phenolic content (424.40 mg/100 g in broccoli sprouts and 497.94 mg/100 g in kale sprouts)”

✓ located verbatim in the source · abstract, characters 690–786 · show in text ↑
t0

“Germination as a Sustainable Green Pre-Treatment for the Recovery and Enhancement of High-Value Compounds in Broccoli and Kale”

✓ located verbatim in the source · title, characters 0–126 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Germination increased essential minerals in both species.

Treatment
Germinationt0
Outcome
essential mineralse0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. viridis
Brassica oleracea var. viridis · TaxonAnn Perry · Public domain · Wikimedia Commons ↗
Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“and essential minerals, while reducing phytic acid levels in both species (up to 82.20%)”

✓ located verbatim in the source · abstract, characters 788–876 · show in text ↑
t0

“Germination as a Sustainable Green Pre-Treatment for the Recovery and Enhancement of High-Value Compounds in Broccoli and Kale”

✓ located verbatim in the source · title, characters 0–126 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Germination reduced phytic acid levels in both species by up to 82.20%.

Treatment
Germinationt0
Outcome
phytic acid levelse0
Direction
reducinge0
Value
up to 82.20%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. viridis
Brassica oleracea var. viridis · TaxonAnn Perry · Public domain · Wikimedia Commons ↗
Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“reducing phytic acid levels in both species (up to 82.20%)”

✓ located verbatim in the source · abstract, characters 818–876 · show in text ↑
t0

“Germination as a Sustainable Green Pre-Treatment for the Recovery and Enhancement of High-Value Compounds in Broccoli and Kale”

✓ located verbatim in the source · title, characters 0–126 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Broccoli and kale seed powders showed antimicrobial effects.

Organ / stage
seed powderse0
Outcome
Antimicrobial effectse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. viridis
Brassica oleracea var. viridis · TaxonAnn Perry · Public domain · Wikimedia Commons ↗
Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Antimicrobial effects were matrix-dependent, being detected in broccoli and kale seed powders”

✓ located verbatim in the source · abstract, characters 878–971 · show in text ↑
t0

“Broccoli and Kale”

✓ located verbatim in the source · title, characters 109–126 · show in text ↑
Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

Broccoli and kale sprout powders showed no inhibitory activity under tested conditions.

Organ / stage
sprout powderse0
Outcome
no inhibitory activitye0
Qualifier
under the tested conditionse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. viridis
Brassica oleracea var. viridis · TaxonAnn Perry · Public domain · Wikimedia Commons ↗
Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“no inhibitory activity was observed for the corresponding sprout powders under the tested conditions”

✓ located verbatim in the source · abstract, characters 979–1079 · show in text ↑
t0

“Broccoli and Kale”

✓ located verbatim in the source · title, characters 109–126 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

FTIR indicated changes to carbohydrate, protein and phenolic functional groups after germination.

Outcome
modifications in functional groups related to carbohydrates, proteins, and phenolic compoundse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. viridis
Brassica oleracea var. viridis · TaxonAnn Perry · Public domain · Wikimedia Commons ↗
Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“FTIR spectra indicated notable modifications in functional groups related to carbohydrates, proteins, and phenolic compounds”

✓ located verbatim in the source · abstract, characters 1081–1205 · show in text ↑
t0

“Broccoli and Kale”

✓ located verbatim in the source · title, characters 109–126 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

SAXS analysis revealed nanoscale structural reorganization after germination.

Outcome
structural reorganizations at the nanoscalee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. viridis
Brassica oleracea var. viridis · TaxonAnn Perry · Public domain · Wikimedia Commons ↗
Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“SAXS analysis revealed structural reorganizations at the nanoscale”

✓ located verbatim in the source · abstract, characters 1213–1279 · show in text ↑
t0

“Broccoli and Kale”

✓ located verbatim in the source · title, characters 109–126 · show in text ↑

processing · molecular: metabolites · Brassica oleracea (wild cabbage)Positive and negative results

24 papers: 18 positive, 6 negative or no effect

17 papers in the same direction as this paper · 6 in the opposite direction

  1. negative
  2. no effect
  3. negative

20 papers more in this group

See all 24 papers →

processing · quality: phytochemicals · Brassica oleracea (wild cabbage)Positive and negative results

24 papers: 12 positive, 12 negative or no effect

11 papers in the same direction as this paper · 11 in the opposite direction · 1 reporting no difference from an alternative

  1. no effect
  2. negative
  3. negative

20 papers more in this group

See all 24 papers →

processing · quality: protein · Brassica oleracea (wild cabbage)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.