Abstract
Sucrose transporters (SUTs) mediate sucrose movement across plant membranes, playing a crucial role in carbon allocation and stress responses. Although finger millet (Eleusine coracana) is known for its inherent drought resistance, the specific involvement of SUT genes in this characteristic is still unclear. This study aimed to identify the SUT genes of millet and to assess their expression in drought conditions. Five SUT genes (EcSUT1-EcSUT5) were identified that encode proteins with 9-12 transmembrane domains. Phylogenetic analysis clustered these SUT members across all three main SUT groups, suggesting an evolutionary divergence within the family. Synteny analysis revealed conserved genomic regions, with EcSUT2 showing 91-94% identity with orthologs in closely related grasses. Structural models further confirmed their typical transmembrane architecture. Interaction analysis identified EcSUT2 as a key interaction with SWEET transporters. Furthermore, the promoter regions of EcSUT2 and EcSUT5 were found to be enriched with hormone and stress-responsive elements. Under drought conditions, EcSUT1-EcSUT4 displayed transient induction, while EcSUT5 showed sustained upregulation, especially in the roots, notably after 48 h. The finger millet SUT family exhibits evolutionary conservation within grasses, with individual genes that play different roles in stress response. The persistent upregulation of EcSUT5 under drought strongly suggests its involvement in maintaining sucrose transport during long-term adverse conditions. This candidate gene requires further functional validation to uncover the stress dynamics for sustainable crop improvement.
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Claims 9
Claim · model-written summary
Five EcSUT genes encode proteins with 9-12 transmembrane domains.
- Taxon
- finger millet (Eleusine coracana)
Eleusine coracanat0 - Gene
- EcSUT1-EcSUT5e0
- Outcome
- 9-12 transmembrane domainse0
- Value
- 9-12 transmembrane domainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Five SUT genes (EcSUT1-EcSUT5) were identified that encode proteins with 9-12 transmembrane domains”
t0“finger millet (Eleusine coracana)”
Claim · model-written summary
The SUT members clustered across all three main SUT groups.
- Taxon
- finger millet (Eleusine coracana)Eleusine coracanat0
- Outcome
- clustered these SUT members across all three main SUT groupse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phylogenetic analysis clustered these SUT members across all three main SUT groups”
t0“finger millet (Eleusine coracana)”
Claim · model-written summary
Synteny revealed conserved regions and 91-94% identity of EcSUT2 with grass orthologs.
- Taxon
- finger millet (Eleusine coracana)Eleusine coracanat0
- Gene
- EcSUT2e0
- Outcome
- identity with orthologse0
- Value
- 91-94%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Synteny analysis revealed conserved genomic regions, with EcSUT2 showing 91-94% identity with orthologs in closely related grasses”
t0“finger millet (Eleusine coracana)”
Claim · model-written summary
Structural models confirmed typical transmembrane architecture.
- Taxon
- finger millet (Eleusine coracana)Eleusine coracanat0
- Outcome
- typical transmembrane architecturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Structural models further confirmed their typical transmembrane architecture”
t0“finger millet (Eleusine coracana)”
Claim · model-written summary
EcSUT2 interacted with SWEET transporters.
- Taxon
- finger millet (Eleusine coracana)Eleusine coracanat0
- Gene
- EcSUT2e0
- Outcome
- interaction with SWEET transporterse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Interaction analysis identified EcSUT2 as a key interaction with SWEET transporters”
t0“finger millet (Eleusine coracana)”
Claim · model-written summary
EcSUT2 and EcSUT5 promoters were enriched with hormone and stress-responsive elements.
- Taxon
- finger millet (Eleusine coracana)Eleusine coracanat0
- Gene
- EcSUT2 and EcSUT5e0
- Outcome
- hormone and stress-responsive elementse0
- Direction
- enrichede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the promoter regions of EcSUT2 and EcSUT5 were found to be enriched with hormone and stress-responsive elements”
t0“finger millet (Eleusine coracana)”
Claim · model-written summary
EcSUT1-EcSUT4 showed transient induction under drought.
- Taxon
- finger millet (Eleusine coracana)Eleusine coracanat0
- Treatment
- drought conditionse0
- Gene
- EcSUT1-EcSUT4e0
- Outcome
- transient inductione0
- Direction
- inductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under drought conditions, EcSUT1-EcSUT4 displayed transient induction”
t0“finger millet (Eleusine coracana)”
Claim · model-written summary
EcSUT5 showed sustained upregulation under drought, particularly in roots after 48 h.
- Taxon
- finger millet (Eleusine coracana)Eleusine coracanat0
- Organ / stage
- rootse0
- Gene
- EcSUT5e0
- Outcome
- sustained upregulatione0
- Direction
- upregulatione0
- Qualifier
- after 48 he0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EcSUT5 showed sustained upregulation, especially in the roots, notably after 48 h”
t0“finger millet (Eleusine coracana)”
Claim · model-written summary
Persistent EcSUT5 upregulation suggests a role in maintaining sucrose transport during long-term adverse conditions.
- Taxon
- finger millet (Eleusine coracana)Eleusine coracanat0
- Gene
- EcSUT5e0
- Outcome
- maintaining sucrose transporte0
- Qualifier
- under droughte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The persistent upregulation of EcSUT5 under drought strongly suggests its involvement in maintaining sucrose transport during long-term adverse conditions”
t0“finger millet (Eleusine coracana)”