Abstract
Tea plant ( Camellia sinensis (L.) O. Kuntze) is a cold-sensitive leaf-harvesting crop whose growth, yield, and processed tea quality are all inhibited by low temperatures. Therefore, identifying the regulatory genes involved in tea plant growth and freezing tolerance is crucial for genetic improvement. WRKY transcription factors regulate various plant processes, including growth and development, stress responses, and metabolite biosynthesis. However, the molecular network through which WRKY coordinates these pathways in tea plants remains unclear. In this study, we revealed that CsWRKY57L , a cold-inducible WRKY IIc subfamily member, positively regulated freezing tolerance by directly promoting flavonoid accumulation in tea plants. Transient suppression of CsWRKY57L weakened the freezing tolerance of tea plants by reducing flavonoid content and suppressing the C-repeat-binding factor ( CBF ) - cold-responsive ( COR ) gene pathway. In contrast, heterologous overexpression of CsWRKY57L in Arabidopsis had the opposite effect. Additionally, overexpression of CsWRKY57L inhibited reproductive development and accelerated senescence in Arabidopsis . Interaction analysis revealed that CsWRKY57L directly binds to the promoters of CsSWEET1a , CsSWEET15 , and AtSWEET15 , which encode sugar transporters essential for plant reproductive development, and inhibits their transcription. Overall, the study revealed a dual role of CsWRKY57L in promoting freezing tolerance via flavonoid biosynthesis and inhibiting reproductive development by regulating SWEETs expression. This study uncovers a novel mechanism whereby CsWRKY57L coordinately regulates both stress responses and growth in tea plants, providing a molecular basis for breeding low-temperature-tolerant varieties with restricted reproductive development.
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Claims 8
Claim · model-written summary
CsWRKY57L positively regulates freezing tolerance in tea plants by directly promoting flavonoid accumulation.
Verbatim
e1“CsWRKY57L , a cold-inducible WRKY IIc subfamily member, positively regulated freezing tolerance by directly promoting flavonoid accumulation in tea plants”
Claim · model-written summary
Transient suppression of CsWRKY57L weakened freezing tolerance in tea plants.
- Taxon
- tea plantse2
- Treatment
- Transient suppression of CsWRKY57Le2
- Gene
- CsWRKY57Le2
- Outcome
- freezing tolerancee2
- Direction
- weakenede2
Not reportedComparator
Verbatim
e2“Transient suppression of CsWRKY57L weakened the freezing tolerance of tea plants by reducing flavonoid content and suppressing the C-repeat-binding factor ( CBF ) - cold-responsive ( COR ) gene pathway”
Claim · model-written summary
Transient suppression of CsWRKY57L reduced flavonoid content in tea plants.
- Taxon
- tea plantse2
- Treatment
- Transient suppression of CsWRKY57Le2
- Outcome
- flavonoid contente2
- Direction
- reducinge2
Not reportedComparator
Verbatim
e2“Transient suppression of CsWRKY57L weakened the freezing tolerance of tea plants by reducing flavonoid content and suppressing the C-repeat-binding factor ( CBF ) - cold-responsive ( COR ) gene pathway”
Claim · model-written summary
Transient suppression of CsWRKY57L suppressed the CBF-COR gene pathway in tea plants.
- Taxon
- tea plantse2
- Treatment
- Transient suppression of CsWRKY57Le2
- Outcome
- C-repeat-binding factor ( CBF ) - cold-responsive ( COR ) gene pathwaye2
- Direction
- suppressinge2
Not reportedComparator
Verbatim
e2“Transient suppression of CsWRKY57L weakened the freezing tolerance of tea plants by reducing flavonoid content and suppressing the C-repeat-binding factor ( CBF ) - cold-responsive ( COR ) gene pathway”
Claim · model-written summary
Heterologous overexpression of CsWRKY57L in Arabidopsis had the opposite effect on freezing tolerance to suppression in tea plants.
- Taxon
- Arabidopsis
Arabidopsise3 - Treatment
- heterologous overexpression of CsWRKY57Le3
- Outcome
- freezing tolerancee2
- Direction
- opposite effecte3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Transient suppression of CsWRKY57L weakened the freezing tolerance of tea plants by reducing flavonoid content and suppressing the C-repeat-binding factor ( CBF ) - cold-responsive ( COR ) gene pathway”
e3“In contrast, heterologous overexpression of CsWRKY57L in Arabidopsis had the opposite effect”
Claim · model-written summary
Overexpression of CsWRKY57L inhibited reproductive development in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise4
- Treatment
- overexpression of CsWRKY57Le4
- Outcome
- reproductive developmente4
- Direction
- inhibitede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“overexpression of CsWRKY57L inhibited reproductive development and accelerated senescence in Arabidopsis”
Claim · model-written summary
Overexpression of CsWRKY57L accelerated senescence in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise4
- Treatment
- overexpression of CsWRKY57Le4
- Outcome
- senescencee4
- Direction
- acceleratede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“overexpression of CsWRKY57L inhibited reproductive development and accelerated senescence in Arabidopsis”
Claim · model-written summary
CsWRKY57L directly binds the promoters of CsSWEET1a , CsSWEET15 and AtSWEET15 and inhibits their transcription.
Verbatim
e1“CsWRKY57L , a cold-inducible WRKY IIc subfamily member, positively regulated freezing tolerance by directly promoting flavonoid accumulation in tea plants”
e5“CsWRKY57L directly binds to the promoters of CsSWEET1a , CsSWEET15 , and AtSWEET15 , which encode sugar transporters essential for plant reproductive development, and inhibits their transcription”
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