Abstract
Mitogen-activated protein kinase kinase kinase (MAPKKK) assumes a pivotal position within the MAPK cascade, converting external stimuli into intracellular responses and mediating plant stress resistance. However, there are limited reports investigating its function in regulating resistance to soybean mosaic virus (SMV). Here, a MAPKKK2-like gene GmMEKK2 (LOC100798607) was identified from an SMV-resistant cultivar, with an amino acid sequence containing the typical conserved G(T/S)Px(W/Y/F)MAPExV domain, showing homology to AtMEKK1, AtMEKK2 (SUMM1) and OsMAPKKK5. Overexpression of GmMEKK2 in soybean reduced SMV accumulation and the disease index, mitigated the yield loss after SMV inoculation and improved yield traits. In contrast, silencing GmMEKK2 via virus-induced gene silencing (VIGS) significantly enhanced SMV susceptibility, with increased disease index and viral content. Transcriptionally, defence responce genes such as PR1s and RPM1s were pre-activated in the overexpression lines before SMV infection, conferring soybean with preventive resistance. While non-transgenic plants showed severe down-regulation of basic metabolic genes and strong induction of genomic repair genes at 14 days post-inoculation (dpi), overexpression lines exhibited minimal changes. Despite lacking kinase activity, GmMEKK2 bound with GmMKK1 and GmMPK4A to block the MKK1/2-MPK4 cascade, thereby indirectly enhancing the salicylic acid-induced defence responses. Additionally, GmMEKK2 overexpression elevated basal reactive oxygen species (ROS) levels to trigger autoimmunity, while maintaining ROS homeostasis via the antioxidant enzyme system in soybean. This study clarifies the function and molecular mechanism of GmMEKK2 in SMV resistance, providing a strategy for improving soybean SMV resistance.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 14
Claim · model-written summary
GmMEKK2 was identified in an SMV resistant cultivar with a conserved domain.
- Taxon
- Soybean
Glycine maxt0 - Interactor
- SMVe0pathogen
- Gene
- GmMEKK2e0
- Outcome
- G(T/S)Px(W/Y/F)MAPExV domaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a MAPKKK2-like gene GmMEKK2 (LOC100798607) was identified from an SMV-resistant cultivar, with an amino acid sequence containing the typical conserved G(T/S)Px(W/Y/F)MAPExV domain”
t0“Soybean”
Claim · model-written summary
GmMEKK2 overexpression reduced SMV accumulation and disease index.
- Taxon
- soybeanGlycine maxe0
- Treatment
- Overexpression of GmMEKK2e0
- Interactor
- SMVe0pathogen
- Gene
- GmMEKK2e0
- Outcome
- SMV accumulation and the disease indexe0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of GmMEKK2 in soybean reduced SMV accumulation and the disease index, mitigated the yield loss after SMV inoculation and improved yield traits.”
Claim · model-written summary
GmMEKK2 overexpression mitigated yield loss after SMV inoculation.
- Taxon
- soybeanGlycine maxe0
- Treatment
- Overexpression of GmMEKK2e0
- Interactor
- SMVe0pathogen
- Gene
- GmMEKK2e0
- Outcome
- yield losse0
- Direction
- mitigatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of GmMEKK2 in soybean reduced SMV accumulation and the disease index, mitigated the yield loss after SMV inoculation and improved yield traits.”
Claim · model-written summary
GmMEKK2 overexpression improved soybean yield traits.
- Taxon
- soybeanGlycine maxe0
- Treatment
- Overexpression of GmMEKK2e0
- Interactor
- SMVe0pathogen
- Gene
- GmMEKK2e0
- Outcome
- yield traitse0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of GmMEKK2 in soybean reduced SMV accumulation and the disease index, mitigated the yield loss after SMV inoculation and improved yield traits.”
Claim · model-written summary
GmMEKK2 silencing increased SMV susceptibility, disease index and viral content.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- silencing GmMEKK2e0
- Interactor
- SMVe0pathogen
- Gene
- GmMEKK2e0
- Outcome
- SMV susceptibilitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing GmMEKK2 via virus-induced gene silencing (VIGS) significantly enhanced SMV susceptibility, with increased disease index and viral content.”
t0“Soybean”
Claim · model-written summary
GmMEKK2 silencing increased disease index and viral content.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- silencing GmMEKK2e0
- Interactor
- SMVe0pathogen
- Gene
- GmMEKK2e0
- Outcome
- disease index and viral contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing GmMEKK2 via virus-induced gene silencing (VIGS) significantly enhanced SMV susceptibility, with increased disease index and viral content.”
t0“Soybean”
Claim · model-written summary
Defense genes PR1s and RPM1s were pre activated in overexpression lines before infection.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- overexpression linese0
- Interactor
- SMVe0pathogen
- Gene
- PR1s and RPM1se0
- Outcome
- defence responce genese0
- Direction
- pre-activatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“defence responce genes such as PR1s and RPM1s were pre-activated in the overexpression lines before SMV infection”
t0“Soybean”
Claim · model-written summary
Nontransgenic plants showed severe downregulation of basic metabolic genes at 14 days after inoculation.
- Taxon
- SoybeanGlycine maxt0
- Material
- non-transgenic plantse0
- Outcome
- basic metabolic genese0
- Direction
- down-regulatione0
- Qualifier
- 14 days post-inoculation (dpi)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“non-transgenic plants showed severe down-regulation of basic metabolic genes and strong induction of genomic repair genes at 14 days post-inoculation (dpi)”
t0“Soybean”
Claim · model-written summary
Nontransgenic plants showed strong induction of genomic repair genes at 14 days after inoculation.
- Taxon
- SoybeanGlycine maxt0
- Material
- non-transgenic plantse0
- Outcome
- genomic repair genese0
- Direction
- inductione0
- Qualifier
- 14 days post-inoculation (dpi)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“non-transgenic plants showed severe down-regulation of basic metabolic genes and strong induction of genomic repair genes at 14 days post-inoculation (dpi)”
t0“Soybean”
Claim · model-written summary
Overexpression lines showed minimal gene expression changes following infection.
- Taxon
- SoybeanGlycine maxt0
- Material
- overexpression linese0
- Outcome
- minimal changese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression lines exhibited minimal changes”
t0“Soybean”
Claim · model-written summary
GmMEKK2 bound GmMKK1 and GmMPK4A and blocked the MKK1/2-MPK4 cascade.
- Taxon
- SoybeanGlycine maxt0
- Gene
- GmMEKK2e0
- Outcome
- MKK1/2-MPK4 cascadee0
- Qualifier
- bound with GmMKK1 and GmMPK4Ae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmMEKK2 bound with GmMKK1 and GmMPK4A to block the MKK1/2-MPK4 cascade, thereby indirectly enhancing the salicylic acid-induced defence responses.”
t0“Soybean”
Claim · model-written summary
GmMEKK2 blockade indirectly enhanced salicylic acid induced defense.
- Taxon
- SoybeanGlycine maxt0
- Gene
- GmMEKK2e0
- Compound
- salicylic acide0
- Outcome
- defence responsese0
- Direction
- enhancinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmMEKK2 bound with GmMKK1 and GmMPK4A to block the MKK1/2-MPK4 cascade, thereby indirectly enhancing the salicylic acid-induced defence responses.”
t0“Soybean”
Claim · model-written summary
GmMEKK2 overexpression increased basal reactive oxygen species to trigger autoimmunity.
- Taxon
- soybeanGlycine maxe0
- Treatment
- GmMEKK2 overexpressione0
- Gene
- GmMEKK2e0
- Outcome
- basal reactive oxygen species (ROS) levelse0
- Direction
- elevatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmMEKK2 overexpression elevated basal reactive oxygen species (ROS) levels to trigger autoimmunity, while maintaining ROS homeostasis via the antioxidant enzyme system in soybean.”
Claim · model-written summary
GmMEKK2 overexpression maintained ROS homeostasis through the antioxidant enzyme system.
- Taxon
- soybeanGlycine maxe0
- Gene
- GmMEKK2e0
- Outcome
- ROS homeostasise0
- Qualifier
- antioxidant enzyme systeme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmMEKK2 overexpression elevated basal reactive oxygen species (ROS) levels to trigger autoimmunity, while maintaining ROS homeostasis via the antioxidant enzyme system in soybean.”
The same questions in other papers
loss of function · disease · Glycine max (soybean)Positive and negative results
19 papers in the same direction as this paper · 17 in the opposite direction
- positive
- positive
- positive
33 papers more in this group
See all 37 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.