Abstract
Microbial fortification represents a promising approach for selenium biofortification in crops. Building on the previous discovery that Bacillus cereus SESY enhances selenium uptake in Brassica napus, this study employed an integrated multi-omics approach to investigate the mechanism by which B. cereus SESY enhances Se bioavailability in the Brassica napus rhizosphere. Inoculation with B. cereus SESY significantly increased selenium content in Brassica napus roots and shoots in calcareous soil by 42.9% and 21.5%, respectively, and increased the selenium content of shoots in yellow brown soil by 30.7%. B. cereus SESY promoted the transformation of residual Se into bioavailable forms and enriched bacterial taxa with high motility and Se-transforming capacity (e.g., Lysobacter, Rhodanobacter, Sphingomonas and Burkholderiaceae) in rhizosphere soil. Key genes of these bacteria involved in Se metabolism (e.g., trxA, narH, cysE, cysK, metB) and cell motility genes (e.g., FlgG, CheW, FliH) were up-regulated. Core rhizosphere metabolites such as N-formylmethionine and xanthine correlated strongly with enriched bacteria abundance and available Se. Joint application of these metabolites with enriched bacteria increased plant Se content by 144% and rhizosphere soil available Se by 13.4%. These results reveal a metabolite-mediated microbial network that enhances Se mobility and plant uptake, providing a novel strategy for microbiome-driven biofortification.
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Claims 10
Claim · model-written summary
Inoculation increased root selenium content by 42.9% in calcareous soil.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napuse0 - Treatment
- Inoculation with B. cereus SESYe0
- Interactor
- B. cereus SESY
Bacillus cereuse0symbiont - Outcome
- selenium content in Brassica napus rootse0
- Direction
- increasede0
- Value
- 42.9%e0
- Setting
- calcareous soile0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“Inoculation with B. cereus SESY significantly increased selenium content in Brassica napus roots and shoots in calcareous soil by 42.9% and 21.5%, respectively”
Claim · model-written summary
Inoculation increased shoot selenium content by 21.5% in calcareous soil.
- Taxon
- Brassica napusBrassica napuse0
- Treatment
- Inoculation with B. cereus SESYe0
- Interactor
- B. cereus SESYBacillus cereuse0symbiont
- Outcome
- selenium content in Brassica napus roots and shootse0
- Direction
- increasede0
- Value
- 21.5%e0
- Setting
- calcareous soile0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inoculation with B. cereus SESY significantly increased selenium content in Brassica napus roots and shoots in calcareous soil by 42.9% and 21.5%, respectively”
Claim · model-written summary
Inoculation increased shoot selenium content by 30.7% in yellow brown soil.
- Taxon
- Brassica napusBrassica napuse1
- Treatment
- B. cereus SESYe1
- Interactor
- B. cereus SESYBacillus cereuse1symbionte0
- Outcome
- selenium content of shootse0
- Direction
- increasede0
- Value
- 30.7%e0
- Setting
- yellow brown soile0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased the selenium content of shoots in yellow brown soil by 30.7%”
t0“Root‐Exuded Metabolites Recruit Selenium‐Transforming Microbiota to Enhance Plant Selenium Acquisition”
e1“Inoculation with B. cereus SESY significantly increased selenium content in Brassica napus roots and shoots in calcareous soil by 42.9% and 21.5%, respectively”
Claim · model-written summary
B. cereus SESY promoted conversion of residual selenium to bioavailable forms.
- Taxon
- Brassica napusBrassica napuse1
- Interactor
- B. cereus SESYBacillus cereuse0symbiont
- Outcome
- transformation of residual Se into bioavailable formse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“B. cereus SESY promoted the transformation of residual Se into bioavailable forms”
t0“Root‐Exuded Metabolites Recruit Selenium‐Transforming Microbiota to Enhance Plant Selenium Acquisition”
e1“Inoculation with B. cereus SESY significantly increased selenium content in Brassica napus roots and shoots in calcareous soil by 42.9% and 21.5%, respectively”
Claim · model-written summary
B. cereus SESY enriched motile selenium-transforming bacteria in rhizosphere soil.
- Taxon
- Brassica napusBrassica napuse1
- Interactor
- Lysobacter
Lysobactere0symbiont - Outcome
- enriched bacterial taxa with high motility and Se-transforming capacitye0
- Qualifier
- Rhodanobacter, Sphingomonas and Burkholderiaceaee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enriched bacterial taxa with high motility and Se-transforming capacity (e.g., Lysobacter, Rhodanobacter, Sphingomonas and Burkholderiaceae) in rhizosphere soil”
t0“Root‐Exuded Metabolites Recruit Selenium‐Transforming Microbiota to Enhance Plant Selenium Acquisition”
e1“Inoculation with B. cereus SESY significantly increased selenium content in Brassica napus roots and shoots in calcareous soil by 42.9% and 21.5%, respectively”
Claim · model-written summary
Selenium metabolism genes of enriched bacteria were up-regulated.
- Taxon
- Brassica napusBrassica napuse1
- Gene
- trxA, narH, cysE, cysK, metBe0
- Outcome
- Key genes of these bacteria involved in Se metabolisme0
- Direction
- up-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Key genes of these bacteria involved in Se metabolism (e.g., trxA, narH, cysE, cysK, metB) and cell motility genes (e.g., FlgG, CheW, FliH) were up-regulated”
t0“Root‐Exuded Metabolites Recruit Selenium‐Transforming Microbiota to Enhance Plant Selenium Acquisition”
e1“Inoculation with B. cereus SESY significantly increased selenium content in Brassica napus roots and shoots in calcareous soil by 42.9% and 21.5%, respectively”
Claim · model-written summary
Cell motility genes of enriched bacteria were up-regulated.
- Taxon
- Brassica napusBrassica napuse1
- Gene
- FlgG, CheW, FliHe0
- Outcome
- cell motility genese0
- Direction
- up-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cell motility genes (e.g., FlgG, CheW, FliH) were up-regulated”
t0“Root‐Exuded Metabolites Recruit Selenium‐Transforming Microbiota to Enhance Plant Selenium Acquisition”
e1“Inoculation with B. cereus SESY significantly increased selenium content in Brassica napus roots and shoots in calcareous soil by 42.9% and 21.5%, respectively”
Claim · model-written summary
N-formylmethionine and xanthine correlated strongly with enriched bacterial abundance and available selenium.
- Taxon
- Brassica napusBrassica napuse1
- Compound
- N-formylmethionine and xanthinee0
- Outcome
- enriched bacteria abundance and available See0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Core rhizosphere metabolites such as N-formylmethionine and xanthine correlated strongly with enriched bacteria abundance and available Se”
t0“Root‐Exuded Metabolites Recruit Selenium‐Transforming Microbiota to Enhance Plant Selenium Acquisition”
e1“Inoculation with B. cereus SESY significantly increased selenium content in Brassica napus roots and shoots in calcareous soil by 42.9% and 21.5%, respectively”
Claim · model-written summary
Joint application of metabolites and enriched bacteria increased plant selenium content by 144%.
- Taxon
- Brassica napusBrassica napuse1
- Treatment
- Joint application of these metabolites with enriched bacteriae0
- Interactor
- enriched bacteriae0symbiont
- Outcome
- plant Se contente0
- Direction
- increasede0
- Value
- 144%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Joint application of these metabolites with enriched bacteria increased plant Se content by 144% and rhizosphere soil available Se by 13.4%”
t0“Root‐Exuded Metabolites Recruit Selenium‐Transforming Microbiota to Enhance Plant Selenium Acquisition”
e1“Inoculation with B. cereus SESY significantly increased selenium content in Brassica napus roots and shoots in calcareous soil by 42.9% and 21.5%, respectively”
Claim · model-written summary
Joint application increased rhizosphere available selenium by 13.4%.
- Taxon
- Brassica napusBrassica napuse1
- Treatment
- Joint application of these metabolites with enriched bacteriae0
- Interactor
- enriched bacteriae0symbiont
- Outcome
- rhizosphere soil available See0
- Direction
- increasede0
- Value
- 13.4%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Joint application of these metabolites with enriched bacteria increased plant Se content by 144% and rhizosphere soil available Se by 13.4%”
t0“Root‐Exuded Metabolites Recruit Selenium‐Transforming Microbiota to Enhance Plant Selenium Acquisition”
e1“Inoculation with B. cereus SESY significantly increased selenium content in Brassica napus roots and shoots in calcareous soil by 42.9% and 21.5%, respectively”
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See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.