Abstract
Rice (Oryza sativa L.), originating from tropical and subtropical regions, is a cold-sensitive and water-demanding crop whose yield and quality are severely compromised by chilling injury or water deficits during growth and development. In this study, we systematically characterized Cold and Drought Resistance 1 (OsCDR1), a nucleus-localized transcription activator belonging to the bZIP transcription factor family. OsCDR1 overexpression enhances rice tolerance to cold and drought stress, whereas knockout mutants of OsCDR1 exhibit reduced resistance to low temperatures and drought conditions. Furthermore, OsCDR1 positively regulates abscisic acid (ABA) signaling in rice, and mediates ABA-regulated drought tolerance responses in rice. Integrated RNA-seq and CUT&Tag analyses demonstrated that OsCDR1 coordinates with ABA-dependent (PIP2;2) and -independent genes (DREB1J). OsCDR1 specifically binds to the G-box cis-element in the promoters of PIP2;2 and DREB1J, thereby activating their transcription and regulating the abiotic stress responses in rice. Protein interaction analysis revealed that OsCDR1 interacts with the kinase OsMPK9 in vivo and in vitro, and a dual-luciferase reporter assay showed that OsMPK9 and OsCDR1 regulate the transcription of DREB1J and PIP2;2 in the same functional pathway. Overexpression of OsMPK9 inhibits ABA responses in rice and reduces cold and drought tolerance. Our findings establish the OsMPK9-OsCDR1 module as a critical hub connecting ABA signaling to abiotic stress resilience, providing new insights for breeding crops.
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Claims 11
Claim · model-written summary
OsCDR1 is a nuclear transcription activator in rice.
- Taxon
- rice
Oryza sativat0 - Gene
- OsCDR1e0
- Outcome
- nucleus-localized transcription activatore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsCDR1), a nucleus-localized transcription activator belonging to the bZIP transcription factor family”
t0“The OsMPK9 – OsCDR1 module regulates abiotic stress responses in rice”
Claim · model-written summary
OsCDR1 overexpression enhances rice cold and drought tolerance.
- Taxon
- riceOryza sativae0
- Treatment
- OsCDR1 overexpressione0
- Gene
- OsCDR1e0
- Outcome
- rice tolerance to cold and drought stresse0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsCDR1 overexpression enhances rice tolerance to cold and drought stress”
Claim · model-written summary
OsCDR1 knockout reduces rice resistance to low temperatures and drought.
- Taxon
- riceOryza sativat0
- Treatment
- knockout mutants of OsCDR1e0
- Gene
- OsCDR1e0
- Outcome
- resistance to low temperatures and drought conditionse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“knockout mutants of OsCDR1 exhibit reduced resistance to low temperatures and drought conditions”
t0“The OsMPK9 – OsCDR1 module regulates abiotic stress responses in rice”
Claim · model-written summary
OsCDR1 positively regulates ABA signaling in rice.
- Taxon
- riceOryza sativae0
- Gene
- OsCDR1e0
- Outcome
- abscisic acid (ABA) signalinge0
- Direction
- positivelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsCDR1 positively regulates abscisic acid (ABA) signaling in rice”
Claim · model-written summary
OsCDR1 mediates ABA-regulated drought tolerance responses.
- Taxon
- riceOryza sativae0
- Gene
- OsCDR1t0
- Outcome
- ABA-regulated drought tolerance responsese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mediates ABA-regulated drought tolerance responses in rice”
t0“The OsMPK9 – OsCDR1 module regulates abiotic stress responses in rice”
Claim · model-written summary
OsCDR1 coordinates with PIP2;2 and DREB1J.
- Taxon
- riceOryza sativat0
- Gene
- OsCDR1e0
- Outcome
- coordinates with ABA-dependent (PIP2;2) and -independent genes (DREB1J)e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsCDR1 coordinates with ABA-dependent (PIP2;2) and -independent genes (DREB1J)”
t0“The OsMPK9 – OsCDR1 module regulates abiotic stress responses in rice”
Claim · model-written summary
OsCDR1 binds G-box elements in PIP2;2 and DREB1J promoters and activates transcription.
- Taxon
- riceOryza sativat0
- Gene
- OsCDR1e0
- Outcome
- activating their transcriptione0
- Qualifier
- binds to the G-box cis-elemente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsCDR1 specifically binds to the G-box cis-element in the promoters of PIP2;2 and DREB1J, thereby activating their transcription”
t0“The OsMPK9 – OsCDR1 module regulates abiotic stress responses in rice”
Claim · model-written summary
OsCDR1 interacts with OsMPK9 in vivo and in vitro.
- Taxon
- riceOryza sativat0
- Gene
- OsCDR1e0
- Outcome
- interacts with the kinase OsMPK9e0
- Qualifier
- in vivo and in vitroe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsCDR1 interacts with the kinase OsMPK9 in vivo and in vitro”
t0“The OsMPK9 – OsCDR1 module regulates abiotic stress responses in rice”
Claim · model-written summary
OsMPK9 and OsCDR1 regulate DREB1J and PIP2;2 transcription in the same pathway.
- Taxon
- riceOryza sativat0
- Gene
- OsMPK9 and OsCDR1e0
- Outcome
- transcription of DREB1J and PIP2;2e0
- Qualifier
- same functional pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsMPK9 and OsCDR1 regulate the transcription of DREB1J and PIP2;2 in the same functional pathway”
t0“The OsMPK9 – OsCDR1 module regulates abiotic stress responses in rice”
Claim · model-written summary
OsMPK9 overexpression inhibits rice ABA responses.
- Taxon
- riceOryza sativae0
- Treatment
- Overexpression of OsMPK9e0
- Gene
- OsMPK9e0
- Outcome
- ABA responsese0
- Direction
- inhibitse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of OsMPK9 inhibits ABA responses in rice”
Claim · model-written summary
OsMPK9 overexpression reduces rice cold and drought tolerance.
- Taxon
- riceOryza sativae0
- Treatment
- Overexpression of OsMPK9e0
- Gene
- OsMPK9e0
- Outcome
- cold and drought tolerancee0
- Direction
- reducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of OsMPK9 inhibits ABA responses in rice and reduces cold and drought tolerance”