Abstract
1. Grafting attenuates drought damage via enhanced phytohormones and antioxidant enzymes with reduced oxidative stress. 2. Transcriptomic profiling revealed drought-responsive DEGs. 3. VvMYBPA1 overexpression confers drought tolerance validated in Arabidopsis and grape callus, revealing novel regulatory mechanisms. Drought stress negatively affects grapevine growth and development. Grafting with rootstock is widely used to improve the quality of grape fruits and confer drought stress tolerance, but the underlying genetics and regulatory mechanism is unclear. Hence, we investigated the physiologic and transcriptomic profiles in the leaves of grafted SM/1103P (SM shoot/1103P root) and self-rooted SM (Shine Muscat) as well as roots of grafted SM/1103P and self-rooted 1103P under drought stress conditions. The results indicated that grafted grapevine effectively attenuated drought damage in grape leaves by increasing phytohormone levels and antioxidant enzyme activity, reducing H 2 O 2 and MDA content. Transcriptomic profiling revealed a total of 11,855 and 11,197 differentially expressed genes (DEGs) were identified in grape leaves and roots respectively. Weighted correlation network analysis (WGCNA) was performed based on the RNA-seq data, and five modules (greenyellow, black, turquoise, salmon and blue) were significantly correlated to drought stress. Pathway analysis showed that DEGs were enriched in the plant hormone signal transduction and MAPK signaling pathway. 916 transcription factor genes (TFs) belonging to different gene families were detected that may participate in regulating the drought stress. Quantitative real-time polymerase chain expression analysis of twelve drought stress responsive DEGs were used to verify the transcriptome data. Furthermore, overexpression of VvMYBPA1 in Arabidopsis thaliana and grape callus improved drought tolerance. Our findings provided new insights into to the regulation of mechanism for improving grapevine adaptation to drought.
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Claims 11
Claim · model-written summary
Grafting attenuated drought damage in grape leaves.
VitisNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“grafted grapevine effectively attenuated drought damage in grape leaves”
t0“grapevine”
Claim · model-written summary
Grafting increased phytohormone levels and antioxidant enzyme activity in drought-stressed grape leaves.
- Treatment
- grafted grapevinee0
- Outcome
- phytohormone levels and antioxidant enzyme activitye0
- Direction
- increasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“grafted grapevine effectively attenuated drought damage in grape leaves by increasing phytohormone levels and antioxidant enzyme activity, reducing H 2 O 2 and MDA content.”
t0“grapevine”
Claim · model-written summary
Grafting reduced H 2 O 2 and MDA content in drought-stressed grape leaves.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“grafted grapevine effectively attenuated drought damage in grape leaves by increasing phytohormone levels and antioxidant enzyme activity, reducing H 2 O 2 and MDA content.”
t0“grapevine”
Claim · model-written summary
Transcriptomics identified 11,855 differentially expressed genes in grape leaves.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“11,855 and 11,197 differentially expressed genes (DEGs) were identified in grape leaves and roots respectively.”
t0“grapevine”
Claim · model-written summary
Transcriptomics identified 11,197 differentially expressed genes in grape roots.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“11,855 and 11,197 differentially expressed genes (DEGs) were identified in grape leaves and roots respectively.”
t0“grapevine”
Claim · model-written summary
Five coexpression modules correlated significantly with drought stress.
- Treatment
- drought stresse0
- Outcome
- five modulese0
- Qualifier
- greenyellow, black, turquoise, salmon and bluee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“five modules (greenyellow, black, turquoise, salmon and blue) were significantly correlated to drought stress.”
t0“grapevine”
Claim · model-written summary
Differentially expressed genes were enriched in plant hormone signal transduction and MAPK signaling pathways.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DEGs were enriched in the plant hormone signal transduction and MAPK signaling pathway.”
t0“grapevine”
Claim · model-written summary
The analysis detected 916 transcription factor genes potentially involved in drought stress regulation.
- Outcome
- transcription factor genes (TFs)e0
- Value
- 916e0
- Qualifier
- may participate in regulating the drought stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“916 transcription factor genes (TFs) belonging to different gene families were detected that may participate in regulating the drought stress.”
t0“grapevine”
Claim · model-written summary
Expression analysis of twelve drought responsive DEGs verified the transcriptome data.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Quantitative real-time polymerase chain expression analysis of twelve drought stress responsive DEGs were used to verify the transcriptome data.”
t0“grapevine”
Claim · model-written summary
VvMYBPA1 overexpression improved drought tolerance in Arabidopsis thaliana.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- overexpression of VvMYBPA1e0
- Gene
- VvMYBPA1e0
- Outcome
- drought tolerancee0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of VvMYBPA1 in Arabidopsis thaliana and grape callus improved drought tolerance.”
Claim · model-written summary
VvMYBPA1 overexpression improved drought tolerance in grape callus.
- Organ / stage
- grape calluse0
- Treatment
- overexpression of VvMYBPA1e0
- Gene
- VvMYBPA1e0
- Outcome
- drought tolerancee0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of VvMYBPA1 in Arabidopsis thaliana and grape callus improved drought tolerance.”
t0“grapevine”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.