Paper · 2025 · ACS Food Science & Technology

Occurrence and Quantification of Fusarium Mycotoxins in Wheat Grains from Uruguay

Cinthia Pendás; Agustina Muela; Gianella Brancatti; et al.

9
claims
18
verbatim quotes
0
citations
2
research areas
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Not open access

Abstract

Fusarium head blight (FHB) is a major limitation for wheat production in Uruguay. This study aimed to optimize a method for the determination of mycotoxins produced by Fusarium in wheat grains. A validated HPLC-MS/MS method was used to analyze zearalenone (ZEA) and type B trichothecenes─deoxynivalenol (DON), 3-acetyldeoxynivalenol (3ADON), 15-acetyldeoxynivalenol (15ADON), and nivalenol (NIV)─in 71 commercial samples from the 2018 season across various cultivars and locations. Mycotoxins were detected in 47 samples, with DON being the most prevalent (46/47), ranging from 102 to 10,507 μg/kg. NIV was quantified in 8 samples (37–66 μg/kg), ZEA in 6 (104–5193 μg/kg), and 3ADON in one sample (67 μg/kg); 15ADON was not detected. FHB-susceptible cultivars showed more positive samples and higher DON and ZEA levels, while moderately resistant cultivars generally had lower concentrations. However, no significant differences in DON content were observed among cultivars.

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Claims 9

Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Mycotoxins occurred in 47 wheat samples.

Outcome
Mycotoxinse0
Value
47 samplese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Mycotoxins were detected in 47 samples”

✓ located verbatim in the source · abstract, characters 482–520 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 56–61 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

DON was present in 46 of 47 positive samples, at 102 to 10,507 μg/kg.

Outcome
DONe0
Value
102 to 10,507 μg/kge0
Qualifier
46/47e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“DON being the most prevalent (46/47), ranging from 102 to 10,507 μg/kg.”

✓ located verbatim in the source · abstract, characters 527–598 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 56–61 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

NIV was quantified in eight samples at 37–66 μg/kg.

Outcome
NIVe0
Value
37–66 μg/kge0
Qualifier
8 samplese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“NIV was quantified in 8 samples (37–66 μg/kg)”

✓ located verbatim in the source · abstract, characters 599–644 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 56–61 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

ZEA was quantified in six samples at 104–5193 μg/kg.

Outcome
ZEAe0
Value
104–5193 μg/kge0
Qualifier
6e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“ZEA in 6 (104–5193 μg/kg)”

✓ located verbatim in the source · abstract, characters 646–671 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 56–61 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

3ADON was quantified in one sample at 67 μg/kg.

Outcome
3ADONe0
Value
67 μg/kge0
Qualifier
one samplee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“3ADON in one sample (67 μg/kg)”

✓ located verbatim in the source · abstract, characters 677–707 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 56–61 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

15ADON was not detected.

Outcome
15ADON was not detectede0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“15ADON was not detected.”

✓ located verbatim in the source · abstract, characters 709–733 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 56–61 · show in text ↑
Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

FHB-susceptible cultivars had more toxin-positive samples.

Material
FHB-susceptible cultivarse0
Outcome
positive samplese0
Direction
moree0
Comparator
moderately resistant cultivarse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“FHB-susceptible cultivars showed more positive samples and higher DON and ZEA levels, while moderately resistant cultivars generally had lower concentrations.”

✓ located verbatim in the source · abstract, characters 734–892 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 56–61 · show in text ↑
Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

FHB-susceptible cultivars had higher DON and ZEA levels than moderately resistant cultivars.

Material
FHB-susceptible cultivarse0
Outcome
DON and ZEA levelse0
Direction
highere0
Comparator
moderately resistant cultivarse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“FHB-susceptible cultivars showed more positive samples and higher DON and ZEA levels, while moderately resistant cultivars generally had lower concentrations.”

✓ located verbatim in the source · abstract, characters 734–892 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 56–61 · show in text ↑
Associationobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

DON content did not differ significantly among cultivars.

Material
cultivarse0
Outcome
DON contente0
Direction
no significant differencese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“no significant differences in DON content were observed among cultivars.”

✓ located verbatim in the source · abstract, characters 902–974 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 56–61 · show in text ↑