Abstract
Plants face the constant challenge of reconciling antagonistic environmental signals, such as nutrient-driven growth and water deficit–induced stress responses. However, the molecular mechanisms that integrate these conflicting pathways remain poorly understood. Through a comprehensive transcriptomic meta-analysis in Arabidopsis thaliana , we show that nitrogen (N) supply and water deficit signaling exhibit overlapping and often opposing gene expression responses. Regulatory network modeling identifies the NIN-LIKE PROTEIN 7 (NLP7) transcription factor (TF) as a central integrator of these convergent transcriptional responses. Through combinatorial water deficit and N supply treatments in wild-type and nlp7 mutant plants, we find that NLP7 accounts for 85% of the transcriptional interaction between these pathways. Chromatin immunoprecipitation and sequencing and a cell TF assay to detect TF regulation genome-wide reveal that NLP7 directly downregulate the expression of TFs such as HB6 , NAC6 , NAP , and WRKY18 , which are central regulators of water deficit–mediated stress signaling. Repression of these secondary TFs has distinct downstream effects on gene expression, influencing shared and water deficit–specific responses. Loss of NLP7 enhances water deficit tolerance, characterized by increased water retention, reduced abscisic acid-mediated stomatal aperture, and altered expression of stress-responsive genes. These findings establish NLP7 as a central hub balancing growth and stress responses, providing insight into how plants integrate competing environmental cues.
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Claims 10
Claim · model-written summary
NLP7 accounts for 85% of the transcriptional interaction between nitrogen and water deficit pathways in Arabidopsis thaliana
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Material
- nlp7 mutante3
- Treatment
- combinatorial water deficit and N supply treatmentse3
- Gene
- NLP7e2
- Outcome
- transcriptional interaction between these pathwayse2
- Value
- 85%e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Through a comprehensive transcriptomic meta-analysis in Arabidopsis thaliana , we show that nitrogen (N) supply and water deficit signaling exhibit overlapping and often opposing gene expression responses”
e2“we find that NLP7 accounts for 85% of the transcriptional interaction between these pathways”
e3“Through combinatorial water deficit and N supply treatments in wild-type and nlp7 mutant plants”
Claim · model-written summary
Nitrogen supply and water deficit signaling show overlapping and often opposing gene expression responses in Arabidopsis thaliana
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Outcome
- gene expression responsese0
- Direction
- overlapping and often opposinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Through a comprehensive transcriptomic meta-analysis in Arabidopsis thaliana , we show that nitrogen (N) supply and water deficit signaling exhibit overlapping and often opposing gene expression responses”
Claim · model-written summary
NLP7 is identified by regulatory network modeling as a central integrator of convergent transcriptional responses
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- NIN-LIKE PROTEIN 7 (NLP7)e1
- Outcome
- central integrator of these convergent transcriptional responsese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Through a comprehensive transcriptomic meta-analysis in Arabidopsis thaliana”
e1“Regulatory network modeling identifies the NIN-LIKE PROTEIN 7 (NLP7) transcription factor (TF) as a central integrator of these convergent transcriptional responses”
Claim · model-written summary
NLP7 directly downregulates the expression of HB6, NAC6 and NAP
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- HB6 , NAC6 , NAPe4
- Outcome
- expression of TFse4
- Direction
- downregulatee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Through a comprehensive transcriptomic meta-analysis in Arabidopsis thaliana”
e4“NLP7 directly downregulate the expression of TFs such as HB6 , NAC6 , NAP , and WRKY18”
Claim · model-written summary
NLP7 directly downregulates the expression of WRKY18
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- WRKY18e4
- Outcome
- expression of TFse4
- Direction
- downregulatee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Through a comprehensive transcriptomic meta-analysis in Arabidopsis thaliana”
e4“NLP7 directly downregulate the expression of TFs such as HB6 , NAC6 , NAP , and WRKY18”
Claim · model-written summary
Repression of secondary TFs by NLP7 has distinct downstream effects on gene expression
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Outcome
- downstream effects on gene expressione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Through a comprehensive transcriptomic meta-analysis in Arabidopsis thaliana”
e5“Repression of these secondary TFs has distinct downstream effects on gene expression”
Claim · model-written summary
Loss of NLP7 enhances water deficit tolerance relative to wild-type plants
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Loss of NLP7e6
- Outcome
- water deficit tolerancee6
- Direction
- enhancese6
- Comparator
- wild-typee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Through a comprehensive transcriptomic meta-analysis in Arabidopsis thaliana”
e3“Through combinatorial water deficit and N supply treatments in wild-type and nlp7 mutant plants”
e6“Loss of NLP7 enhances water deficit tolerance, characterized by increased water retention, reduced abscisic acid-mediated stomatal aperture, and altered expression of stress-responsive genes”
Claim · model-written summary
Loss of NLP7 increases water retention relative to wild-type plants
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Loss of NLP7e6
- Outcome
- water retentione6
- Direction
- increasede6
- Comparator
- wild-typee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Through a comprehensive transcriptomic meta-analysis in Arabidopsis thaliana”
e3“Through combinatorial water deficit and N supply treatments in wild-type and nlp7 mutant plants”
e6“Loss of NLP7 enhances water deficit tolerance, characterized by increased water retention, reduced abscisic acid-mediated stomatal aperture, and altered expression of stress-responsive genes”
Claim · model-written summary
Loss of NLP7 reduces ABA-mediated stomatal aperture relative to wild-type plants
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Loss of NLP7e6
- Outcome
- abscisic acid-mediated stomatal aperturee6
- Direction
- reducede6
- Comparator
- wild-typee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Through a comprehensive transcriptomic meta-analysis in Arabidopsis thaliana”
e3“Through combinatorial water deficit and N supply treatments in wild-type and nlp7 mutant plants”
e6“Loss of NLP7 enhances water deficit tolerance, characterized by increased water retention, reduced abscisic acid-mediated stomatal aperture, and altered expression of stress-responsive genes”
Claim · model-written summary
Loss of NLP7 alters expression of stress-responsive genes under water deficit
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Outcome
- expression of stress-responsive genese6
- Direction
- alterede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Through a comprehensive transcriptomic meta-analysis in Arabidopsis thaliana”
e6“Loss of NLP7 enhances water deficit tolerance, characterized by increased water retention, reduced abscisic acid-mediated stomatal aperture, and altered expression of stress-responsive genes”