Abstract
The Gretchen Hagen 3 (GH3) gene family, a key component of the early auxin-responsive gene family, plays a pivotal role in regulating plant growth, development, and stress responses. However, to date, a comprehensive genome-wide analysis of the GH3 gene family and its potential role in plant defense against viruses, such as tomato yellow leaf curl virus (TYLCV), has not been conducted in Nicotiana benthamiana. Here, the GH3 gene family was thoroughly examined in N. benthamiana using a comprehensive genome-wide bioinformatic approach. A total of 25 potential GH3 genes were discovered in N. benthamiana. Phylogenetic analysis classified these NbGH3s into three different clades. Chromosomal distribution and synteny analyses revealed that NbGH3s are unevenly distributed across 14 chromosomes, with 20 segmental and one tandem duplication pairs. Promoter analysis suggested their involvement in phytohormone signaling and stress responses. Quantitative PCR showed that several NbGH3s are transcriptionally responsive to TYLCV infection, with five of them significantly upregulated in infected leaves. Furthermore, virus-induced gene silencing revealed that the suppression of NbGH3-3 and NbGH3-9 markedly increased host susceptibility to TYLCV, underscoring their critical roles in plant antiviral defense mechanisms. This research establishes a framework for understanding the functions of NbGH3s in plant growth and their response to TYLCV infection.
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Claims 9
Claim · model-written summary
A total of 25 potential GH3 genes were identified in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianat0 - Outcome
- potential GH3 genese1
- Value
- 25e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Family in Nicotiana benthamiana”
e1“A total of 25 potential GH3 genes were discovered in N. benthamiana”
Claim · model-written summary
NbGH3s classify into three different phylogenetic clades.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- NbGH3se2
- Outcome
- Phylogenetic analysise2
- Value
- three different cladese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Family in Nicotiana benthamiana”
e2“Phylogenetic analysis classified these NbGH3s into three different clades”
Claim · model-written summary
NbGH3s are unevenly distributed across 14 chromosomes.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- NbGH3se3
- Outcome
- unevenly distributede3
- Value
- 14 chromosomese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Family in Nicotiana benthamiana”
e3“NbGH3s are unevenly distributed across 14 chromosomes”
Claim · model-written summary
NbGH3s include 20 segmental duplication pairs.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Outcome
- duplication pairse4
- Value
- 20 segmentale4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Family in Nicotiana benthamiana”
e4“with 20 segmental and one tandem duplication pairs”
Claim · model-written summary
NbGH3s include one tandem duplication pair.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Outcome
- duplication pairse4
- Value
- one tandeme4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Family in Nicotiana benthamiana”
e4“with 20 segmental and one tandem duplication pairs”
Claim · model-written summary
Promoter analysis suggests NbGH3s are involved in phytohormone signalling and stress responses.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Outcome
- involvement in phytohormone signaling and stress responsese5
- Qualifier
- Promoter analysis suggestede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Family in Nicotiana benthamiana”
e5“Promoter analysis suggested their involvement in phytohormone signaling and stress responses”
Claim · model-written summary
Several NbGH3s are transcriptionally responsive to TYLCV infection.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Interactor
- TYLCVTomato yellow leaf curl viruse6pathogen
- Gene
- NbGH3se6
- Outcome
- transcriptionally responsivee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Family in Nicotiana benthamiana”
e6“Quantitative PCR showed that several NbGH3s are transcriptionally responsive to TYLCV infection”
Claim · model-written summary
Five NbGH3s are significantly upregulated in TYLCV-infected leaves.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Organ / stage
- infected leavese7
- Interactor
- TYLCVTomato yellow leaf curl viruse6pathogen
- Outcome
- transcriptionally responsivee6
- Direction
- significantly upregulatede7
- Value
- fivee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Family in Nicotiana benthamiana”
e6“Quantitative PCR showed that several NbGH3s are transcriptionally responsive to TYLCV infection”
e7“with five of them significantly upregulated in infected leaves”
Claim · model-written summary
Suppression of NbGH3-3 and NbGH3-9 by virus-induced gene silencing markedly increased host susceptibility to TYLCV.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- suppression of NbGH3-3 and NbGH3-9e7
- Interactor
- TYLCVTomato yellow leaf curl viruse7pathogen
- Gene
- NbGH3-3 and NbGH3-9e7
- Outcome
- host susceptibility to TYLCVe7
- Direction
- markedly increasede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Family in Nicotiana benthamiana”
e7“virus-induced gene silencing revealed that the suppression of NbGH3-3 and NbGH3-9 markedly increased host susceptibility to TYLCV”
The same questions in other papers
loss of function · Tomato yellow leaf curl virus · Nicotiana benthamianaPositive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
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