Abstract
Increasing dietary fibre (DF) intake is an important target to improve health. An attractive strategy for this is to increase DF in wheat which is derived principally from the endosperm cell wall polysaccharide arabinoxylan (AX). The water-extractable form of this (WE-AX) accounts for most soluble dietary fibre (SDF), which is believed to confer particular health benefits. A region of chromosome 6B in some wheat varieties confers high SDF and here we show that the cause is an allele encoding a peroxidase family protein with a single residue change (PER1-v) associated with high WE-AX, compared to the more common form (PER1). Both wheat lines carrying this natural PER1-v variant and those with an induced knockout mutation of PER1 showed reduced dimerization of endosperm ferulate consistent with a mechanism of decreased cross-linking in the cell wall that increases WE-AX. Transiently expressed PER1_RFP fusion protein driven by the native promoter in wheat endosperm was shown to localise to cell walls, whereas PER1-v_RFP did not. We therefore propose that PER1-v lacks the capacity to dimerise AX ferulate in vivo due to mis-localisation caused by the missense single-nucleotide polymorphism (SNP) in the PER1-v allele, so that the SNP acts as a perfect marker. This marker can be used to identify current wheat varieties with high WE-AX to be used by processors and by breeders to ensure future varieties have high WE-AX to make healthier wheat-based foods.
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Claims 5
Claim · model-written summary
The PER1-v allele is associated with high WE-AX compared with PER1 in wheat.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Allele in Wheat”
e1“a peroxidase family protein with a single residue change (PER1-v) associated with high WE-AX, compared to the more common form (PER1)”
Claim · model-written summary
Wheat lines with the PER1-v variant or a PER1 knockout showed reduced dimerization of endosperm ferulate.
- Taxon
- wheat
Triticum aestivume1 - Organ / stage
- endosperme1
- Treatment
- induced knockout mutation of PER1e1
- Gene
- PER1e1
- Outcome
- dimerization of endosperm ferulatee1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Both wheat lines carrying this natural PER1-v variant and those with an induced knockout mutation of PER1 showed reduced dimerization of endosperm ferulate”
Claim · model-written summary
Transiently expressed PER1 fusion protein localised to cell walls in wheat endosperm, whereas PER1-v did not.
- Taxon
- wheatTriticum aestivume1
- Organ / stage
- endosperme1
- Gene
- PER1-v_RFPe1
- Outcome
- localise to cell wallse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Transiently expressed PER1_RFP fusion protein driven by the native promoter in wheat endosperm was shown to localise to cell walls, whereas PER1-v_RFP did not”
Claim · model-written summary
PER1-v likely lacks the capacity to dimerise AX ferulate in vivo because of SNP-caused mis-localisation.
- Taxon
- WheatTriticum aestivumt0
- Gene
- PER1-ve1
- Outcome
- dimerise AX ferulate in vivoe1
- Qualifier
- mis-localisation caused by the missense single-nucleotide polymorphism (SNP)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Allele in Wheat”
e1“PER1-v lacks the capacity to dimerise AX ferulate in vivo due to mis-localisation caused by the missense single-nucleotide polymorphism (SNP) in the PER1-v allele”
Claim · model-written summary
A SNP marker can identify current wheat varieties with high WE-AX for processors and breeders.
- Taxon
- wheatTriticum aestivume1
- Outcome
- identify current wheat varieties with high WE-AXe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“This marker can be used to identify current wheat varieties with high WE-AX to be used by processors and by breeders”