Abstract
Background: Plant citrate synthase (CSY) is involved in the iron deficiency (−Fe) response and aluminum (Al) detoxification. However, knowledge of CSY function in responding to excess iron (+Fe) or Al stress (+Al) is still limited. Methods: The CDS and promoter of GmCSY3 were isolated from soybean and bioinformatically analyzed. The GmCSY3 expression was detected by qRT-PCR and GUS assay. The growth of GmCSY3 recombinant yeast under +Fe or +Al was detected. The phenotype, CSY activity, citric acid concentration, chlorophyll content, MDA, H2O2, O2− contents, GST, CAT, SOD, and POD activities were examined in GmCSY3 overexpressed and RNAi-suppressed soybean chimeras under +Fe or +Al. Perls and Hematoxylin stained the roots, and the FCR activity was determined. Results: GmCSY3 was induced by +Fe or +Al, but not by −Fe. GmCSY3 enhanced yeast’s acid production and resistance to +Fe or +Al. GmCSY3 overexpression in soybean significantly enhanced CSY activity, promoted growth, alleviated oxidative damage caused by +Fe or +Al, with less free Fe3+ and Al3+, and reduced FCR activity, while GmCSY3 RNAi-suppressed showed the opposite effect. Conclusions: GmCSY3 promotes the process of citrate synthesis, chelates Fe3+ and Al3+, alleviates oxidative damage caused by +Fe or +Al, and modulates iron absorption in plants.
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Claims 10
Claim · model-written summary
GmCSY3 is induced by excess iron or aluminum but not by iron deficiency.
- Taxon
- Soybean
Glycine maxt0 - Treatment
- +Fe or +Ale1
- Gene
- GmCSY3e1
- Outcome
- GmCSY3 expressione2
- Direction
- inducede1
- Comparator
- −Fee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Enhances Soybean Tolerance”
e1“GmCSY3 was induced by +Fe or +Al, but not by −Fe”
e2“The GmCSY3 expression was detected by qRT-PCR and GUS assay”
Claim · model-written summary
GmCSY3 enhanced yeast acid production and resistance to excess iron or aluminum.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- +Fe or +Ale1
- Gene
- GmCSY3e1
- Outcome
- acid production and resistancee1
- Direction
- enhancede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Enhances Soybean Tolerance”
e1“GmCSY3 enhanced yeast’s acid production and resistance to +Fe or +Al”
Claim · model-written summary
GmCSY3 overexpression in soybean significantly enhanced CSY activity.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- GmCSY3 overexpressione1
- Gene
- GmCSY3e1
- Outcome
- CSY activitye1
- Direction
- enhancede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Enhances Soybean Tolerance”
e1“GmCSY3 overexpression in soybean significantly enhanced CSY activity”
Claim · model-written summary
GmCSY3 overexpression promoted soybean growth under excess iron or aluminum.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- GmCSY3 overexpressione1
- Outcome
- growthe2
- Direction
- promotede2
- Qualifier
- caused by +Fe or +Ale2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Enhances Soybean Tolerance”
e1“GmCSY3 overexpression in soybean significantly enhanced CSY activity”
e2“promoted growth, alleviated oxidative damage caused by +Fe or +Al”
Claim · model-written summary
GmCSY3 overexpression alleviated oxidative damage caused by excess iron or aluminum.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- GmCSY3 overexpressione1
- Outcome
- oxidative damagee2
- Direction
- alleviatede2
- Qualifier
- caused by +Fe or +Ale2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Enhances Soybean Tolerance”
e1“GmCSY3 overexpression in soybean significantly enhanced CSY activity”
e2“promoted growth, alleviated oxidative damage caused by +Fe or +Al”
Claim · model-written summary
GmCSY3 overexpression led to less free Fe3+ and Al3+ in soybean under excess iron or aluminum.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- GmCSY3 overexpressione1
- Outcome
- free Fe3+ and Al3+e3
- Direction
- lesse3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Enhances Soybean Tolerance”
e1“GmCSY3 overexpression in soybean significantly enhanced CSY activity”
e3“with less free Fe3+ and Al3+”
Claim · model-written summary
GmCSY3 overexpression reduced ferric chelate reductase (FCR) activity in soybean.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- GmCSY3 overexpressione1
- Outcome
- FCR activitye4
- Direction
- reducede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Enhances Soybean Tolerance”
e1“GmCSY3 overexpression in soybean significantly enhanced CSY activity”
e4“reduced FCR activity”
Claim · model-written summary
GmCSY3 RNAi suppression had the opposite effect to GmCSY3 overexpression.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- GmCSY3 RNAi-suppressede5
- Gene
- GmCSY3e5
- Outcome
- effecte5
- Direction
- oppositee5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Enhances Soybean Tolerance”
e5“GmCSY3 RNAi-suppressed showed the opposite effect”
Claim · model-written summary
GmCSY3 chelates Fe3+ and Al3+.
- Taxon
- SoybeanGlycine maxt0
- Gene
- GmCSY3e6
- Outcome
- chelates Fe3+ and Al3+e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Enhances Soybean Tolerance”
e6“GmCSY3 promotes the process of citrate synthesis, chelates Fe3+ and Al3+”
Claim · model-written summary
GmCSY3 modulates iron absorption in plants.
- Taxon
- SoybeanGlycine maxt0
- Gene
- GmCSY3e6
- Outcome
- iron absorptione7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Enhances Soybean Tolerance”
e6“GmCSY3 promotes the process of citrate synthesis, chelates Fe3+ and Al3+”
e7“modulates iron absorption in plants”
The same questions in other papers
aluminium atom · disease · Glycine max (soybean)Positive and negative results
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- negative
- positive
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iron atom · disease · Glycine max (soybean)Positive and negative results
1 paper in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- no effect
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.