Abstract
The nitrogen fertilizer was overapplied with people’s increased demand for crop yield, but the nitrogen utilization efficiency (NUE) of crops was decreasing. In this study, the differentially expressed genes (DEGs) including the nitrogen absorption, transport, distribution and transcription factors were screened under low nitrogen treatment of 0, 3, and 72 h by the physiological changes and RNA-Seq in rapeseed. The results showed that nitrogen were preferentially allocated to the shoots with the increased glutamine synthetase (GS) and NUE activities and the decreased nitrate reductase (NR) activity and the total nitrogen concentration under low nitrogen treatment. The analysis of Gene Ontology (GO) enrichment and the Kyoto encyclopedia of genes and genomes (KEGG) metabolic pathway showed that DEGs of the shoots were mainly involved in metabolic process, protein binding, ion binding, and anion binding, while DEGs of the roots were mainly involved in molecular function, primary metabolic process, ion binding, and anion binding. The gene expression profile analysis indicated that after low nitrogen treatment for 72 h, the expression of most genes in BnaGLNs increased; the expression of BnaWRKY33s and BnaWRKY70s showed significantly decreased in roots; the expression of most genes in BnaMYB4s, BnaMYB44s, and BnaMYB51s decreased in the roots; the expression of most genes in BnaNIAs family was up-regulated in roots; and in the subfamily of BnaNRT2.1s and BnaNRT3.1s, the expression of BnaA6NRT2.1 (BnaA06g04560D), BnaA6NRT2.1 (BnaA06g04570D), BnaA2NRT3.1 (BnaA02g11760D), and BnaC2NRT3.1 (BnaC02g16150D) increased significantly in roots. At the same time, skipped exon (SE) and mutually exclusive exons (MXE) type occurred in shoots and roots in order to have a better adaptation under low nitrogen stress. In conclusion, the NUE activity was increased by regulating BnaNRTs, BnaGLNs and BnaNIAs genes, and the BnaMYBs, BnaWRKYs genes and alternative splicing favored Brassica napus to adapt the low nitrogen stress.
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Claims 11
Claim · model-written summary
Low nitrogen preferentially allocated nitrogen to shoots.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napust0 - Organ / stage
- shootse0
- Treatment
- low nitrogen treatmente1
- Outcome
- nitrogen were preferentially allocated to the shootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“nitrogen were preferentially allocated to the shoots”
t0“Brassica napus”
e1“under low nitrogen treatment of 0, 3, and 72 h”
Claim · model-written summary
Low nitrogen increased GS and NUE activities.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- low nitrogen treatmente0
- Outcome
- glutamine synthetase (GS) and NUE activitiese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the increased glutamine synthetase (GS) and NUE activities and the decreased nitrate reductase (NR) activity and the total nitrogen concentration under low nitrogen treatment”
t0“Brassica napus”
Claim · model-written summary
Low nitrogen decreased nitrate reductase activity and total nitrogen concentration.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- low nitrogen treatmente0
- Outcome
- nitrate reductase (NR) activity and the total nitrogen concentratione0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the decreased nitrate reductase (NR) activity and the total nitrogen concentration under low nitrogen treatment”
t0“Brassica napus”
Claim · model-written summary
Shoot DEGs were chiefly enriched for metabolic process, protein binding and ion binding.
- Taxon
- Brassica napusBrassica napust0
- Organ / stage
- shootse0
- Outcome
- metabolic process, protein binding, ion binding, and anion bindinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DEGs of the shoots were mainly involved in metabolic process, protein binding, ion binding, and anion binding”
t0“Brassica napus”
Claim · model-written summary
Root DEGs were chiefly enriched for molecular function, primary metabolism and ion binding.
- Taxon
- Brassica napusBrassica napust0
- Organ / stage
- rootse0
- Outcome
- molecular function, primary metabolic process, ion binding, and anion bindinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DEGs of the roots were mainly involved in molecular function, primary metabolic process, ion binding, and anion binding”
t0“Brassica napus”
Claim · model-written summary
Most BnaGLNs genes showed increased expression after 72 hours of low nitrogen.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- low nitrogen treatment for 72 he0
- Gene
- BnaGLNse0
- Outcome
- expression of most genes in BnaGLNse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after low nitrogen treatment for 72 h, the expression of most genes in BnaGLNs increased”
t0“Brassica napus”
Claim · model-written summary
Root BnaWRKY33s and BnaWRKY70s expression decreased significantly.
- Taxon
- Brassica napusBrassica napust0
- Organ / stage
- rootse0
- Treatment
- low nitrogen treatment for 72 he1
- Gene
- BnaWRKY33s and BnaWRKY70se0
- Outcome
- expression of BnaWRKY33s and BnaWRKY70se0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of BnaWRKY33s and BnaWRKY70s showed significantly decreased in roots”
t0“Brassica napus”
e1“after low nitrogen treatment for 72 h”
Claim · model-written summary
Most BnaMYB4s, BnaMYB44s and BnaMYB51s expression decreased in roots.
- Taxon
- Brassica napusBrassica napust0
- Organ / stage
- rootse0
- Treatment
- low nitrogen treatment for 72 he1
- Gene
- BnaMYB4s, BnaMYB44s, and BnaMYB51se0
- Outcome
- expression of most genese0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of most genes in BnaMYB4s, BnaMYB44s, and BnaMYB51s decreased in the roots”
t0“Brassica napus”
e1“after low nitrogen treatment for 72 h”
Claim · model-written summary
Most BnaNIAs family genes were upregulated in roots.
- Taxon
- Brassica napusBrassica napust0
- Organ / stage
- rootse0
- Treatment
- low nitrogen treatment for 72 he1
- Gene
- BnaNIAs familye0
- Outcome
- most genes in BnaNIAs familye0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“most genes in BnaNIAs family was up-regulated in roots”
t0“Brassica napus”
e1“after low nitrogen treatment for 72 h”
Claim · model-written summary
Four named BnaNRT2.1 and BnaNRT3.1 genes increased significantly in roots.
- Taxon
- Brassica napusBrassica napust0
- Organ / stage
- rootse0
- Treatment
- low nitrogen treatment for 72 he1
- Outcome
- expression of BnaA6NRT2.1 (BnaA06g04560D), BnaA6NRT2.1 (BnaA06g04570D), BnaA2NRT3.1 (BnaA02g11760D), and BnaC2NRT3.1 (BnaC02g16150D)e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of BnaA6NRT2.1 (BnaA06g04560D), BnaA6NRT2.1 (BnaA06g04570D), BnaA2NRT3.1 (BnaA02g11760D), and BnaC2NRT3.1 (BnaC02g16150D) increased significantly in roots”
t0“Brassica napus”
e1“after low nitrogen treatment for 72 h”
Claim · model-written summary
Skipped exon and mutually exclusive exon splicing occurred in shoots and roots under low nitrogen.
- Taxon
- Brassica napusBrassica napust0
- Organ / stage
- shoots and rootse0
- Outcome
- skipped exon (SE) and mutually exclusive exons (MXE) typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“skipped exon (SE) and mutually exclusive exons (MXE) type occurred in shoots and roots”
t0“Brassica napus”
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See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.