Abstract
To reveal the fertility switching mechanism of temperature-sensitive sterility line PK3-12S (Brassica rapa L.), the differentially expressed proteins were isolated and identified using anthers of PK3-12S in sterile/fertile conditions by 2-DE and LC-MS/MS mass spectrometry. The expression level variations of differentially expressed genes were examined by RT-PCR in PK3-12 flower buds during sterility/fertile development. The result showed that the sterile anther size of PK3-12 was small with a little abortive pollen in the anther room under high temperature. The trait of fertility transformation was controlled by a pair of recessive alleles. There were 31 differentially expressed proteins with more than two times of the expression level, including six protein spots with increasing expression, 11 protein spots with reduced expression, 12 protein spots with complete inhibition, and two protein spots with induced expressed. Fifteen differentially expressed proteins involved in the cellular processes such as signal transduction pathways, glyoxylate and dicarboxylate metabolism, glycolysis gluconeogenesis, biosynthesis of secondary metabolites, biontheses of amino acids, chorismate biosynthesis, and carbon metabolism pathways were identified by mass spectrometry. The BrrbcL gene, encoding a Rubisco subunit-binding accessory protein, had an open reading frame (ORF) in length of 1095 bp encoded 364 amino acids. Compared with fertile anthers, the expression level of BrrbcL gene, annexin gene (ANN) and BetVI allergen family gene (BetVI) was significantly down-regulated during sterile anthers development, which indicated that these genes maybe participate in the fertility transformation of the thermo-sensitive sterile line PK3-12S.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 12
Claim · model-written summary
Under high temperature the sterile anther of PK3-12 was small, with a little abortive pollen in the anther room.
- Taxon
- Brassica rapa
Brassica rapat0 - Material
- PK3-12e1
- Treatment
- high temperaturee1
- Outcome
- sterile anther sizee1
- Qualifier
- a little abortive pollene1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica rapa L.”
e1“the sterile anther size of PK3-12 was small with a little abortive pollen in the anther room under high temperature”
Claim · model-written summary
The fertility transformation trait was controlled by a pair of recessive alleles.
- Taxon
- Brassica rapaBrassica rapat0
- Gene
- a pair of recessive allelese1
- Outcome
- trait of fertility transformatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica rapa L.”
e1“The trait of fertility transformation was controlled by a pair of recessive alleles”
Claim · model-written summary
31 differentially expressed proteins with more than two-fold expression change were identified between sterile and fertile anthers.
- Taxon
- Brassica rapaBrassica rapat0
- Outcome
- differentially expressed proteinse1
- Value
- 31e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica rapa L.”
e1“There were 31 differentially expressed proteins with more than two times of the expression level”
Claim · model-written summary
Six protein spots showed increased expression.
- Taxon
- Brassica rapaBrassica rapat0
- Outcome
- protein spots with increasing expressione1
- Value
- sixe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica rapa L.”
e1“six protein spots with increasing expression”
Claim · model-written summary
Eleven protein spots showed reduced expression.
- Taxon
- Brassica rapaBrassica rapat0
- Outcome
- protein spots with reduced expressione1
- Value
- 11e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica rapa L.”
e1“11 protein spots with reduced expression”
Claim · model-written summary
Twelve protein spots showed complete inhibition.
- Taxon
- Brassica rapaBrassica rapat0
- Outcome
- protein spots with complete inhibitione1
- Value
- 12e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica rapa L.”
e1“12 protein spots with complete inhibition”
Claim · model-written summary
Two protein spots showed induced expression.
- Taxon
- Brassica rapaBrassica rapat0
- Outcome
- protein spots with induced expressede1
- Value
- twoe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica rapa L.”
e1“two protein spots with induced expressed”
Claim · model-written summary
Fifteen differentially expressed proteins were identified by mass spectrometry as involved in cellular processes including signal transduction, glyoxylate and dicarboxylate metabolism, glycolysis gluconeogenesis, secondary metabolite biosynthesis, amino acid biosynthesis, chorismate biosynthesis and carbon metabolism.
- Taxon
- Brassica rapaBrassica rapat0
- Outcome
- differentially expressed proteins involved in the cellular processese1
- Value
- Fifteene1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica rapa L.”
e1“Fifteen differentially expressed proteins involved in the cellular processes such as signal transduction pathways, glyoxylate and dicarboxylate metabolism, glycolysis gluconeogenesis, biosynthesis of secondary metabolites, biontheses of amino acids, chorismate biosynthesis, and carbon metabolism pathways were identified by mass spectrometry”
Claim · model-written summary
The BrrbcL gene, encoding a Rubisco subunit-binding accessory protein, had an open reading frame of 1095 bp.
- Taxon
- Brassica rapaBrassica rapat0
- Gene
- BrrbcL genee1
- Outcome
- open reading frame (ORF) in lengthe1
- Value
- 1095 bpe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica rapa L.”
e1“The BrrbcL gene, encoding a Rubisco subunit-binding accessory protein, had an open reading frame (ORF) in length of 1095 bp”
Claim · model-written summary
The BrrbcL ORF encoded 364 amino acids.
- Taxon
- Brassica rapaBrassica rapat0
- Gene
- BrrbcL genee1
- Outcome
- amino acidse1
- Value
- 364 amino acidse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica rapa L.”
e1“The BrrbcL gene, encoding a Rubisco subunit-binding accessory protein, had an open reading frame (ORF) in length of 1095 bp encoded 364 amino acids”
Claim · model-written summary
Expression of BrrbcL, annexin (ANN) and BetVI genes was significantly down-regulated during sterile anther development compared with fertile anthers.
- Taxon
- Brassica rapaBrassica rapat0
- Organ / stage
- sterile antherse1
- Gene
- BrrbcL gene, annexin gene (ANN) and BetVI allergen family gene (BetVI)e1
- Outcome
- expression levele1
- Direction
- down-regulatede1
- Comparator
- fertile antherse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica rapa L.”
e1“Compared with fertile anthers, the expression level of BrrbcL gene, annexin gene (ANN) and BetVI allergen family gene (BetVI) was significantly down-regulated during sterile anthers development”
Claim · model-written summary
The authors suggest that these down-regulated genes may participate in fertility transformation of the thermo-sensitive sterile line PK3-12S.
- Taxon
- Brassica rapaBrassica rapat0
- Material
- PK3-12Se1
- Outcome
- fertility transformatione1
- Qualifier
- maybe participatee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica rapa L.”
e1“these genes maybe participate in the fertility transformation of the thermo-sensitive sterile line PK3-12S”