Abstract
The yield and quality of rapeseed are significantly affected by waterlogging. Many researches on waterlogging of rapeseed mostly aimed at its mechanism and physiological response, but a few of them identified the genes related to waterlogging. In this study, 248 Brassica napus L. accessions were planted in flowerpots with treatments of waterlogging and well watering for four weeks when the plants had four true leaves in rain-proof installation, and eight waterlogging-responding traits were investigated. The genome-wide association analysis was carried out based on 60K Illumina Infinium SNP genotype data and the candidate genes associated with waterlogging responses were predicted. Under waterlogging, the number of green leaf, the shoot fresh weight and dry weight, the leaf soluble protein content were lower, and the leaf POD activity, the MDA content were higher than those under well watering. The root fresh weight and dry weight were lower in most accessions and higher in some accessions under waterlogging stress. A total of 36 SNPs were identified associated with waterlogging traits and explained the phenotypic variation of 8.28%-12.95%. There were significant (P < 0.05) or extremely significant (P < 0.01) differences in the waterlogging resistance index among genotypes of 17 SNPs. The Blocks containing 17 SNPs covered 71 candidate genes. Among them, 64 candidate genes were homologous to Arabidopsis genes by blast. They encoded transcription factors (e.g. transcription initiation factor IIF, bZIP68, myb-like HTH transcriptional regulator family protein), transport protein (e.g. SUC2), inhibitor of growth (e.g. ING2), protein phosphatase (e.g. protein phosphatase 2C family protein), DNA/RNA binding proteins [e.g. bHLH DNA-binding superfamily protein, RNA-binding (RRM/RBD/RNP motifs) family protein], hormone response proteins (e.g. ARF8, ADC2, ERD6, NF-YC9), oxidative/osmotic/cold stress or water deprivation response proteins (e.g. ERD6, NP1, TIR-NBS-LRR, CRT1b). These results would be benefit for revealing the waterlogging resistance mechanism and cultivating new varieties with waterlogging resistance in Brassica napus L.
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Claims 12
Claim · model-written summary
Waterlogging made number of green leaf lower than well watering.
- Taxon
- Brassica napus L.
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napust0 - Treatment
- waterlogginge0
- Outcome
- number of green leafe0
- Direction
- lowere0
- Comparator
- well wateringe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“Under waterlogging, the number of green leaf, the shoot fresh weight and dry weight, the leaf soluble protein content were lower, and the leaf POD activity, the MDA content were higher than those under well watering.”
t0“Brassica napus L.”
Claim · model-written summary
Waterlogging made shoot fresh weight and dry weight lower than well watering.
- Taxon
- Brassica napus L.Brassica napust0
- Treatment
- waterlogginge0
- Outcome
- shoot fresh weight and dry weighte0
- Direction
- lowere0
- Comparator
- well wateringe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under waterlogging, the number of green leaf, the shoot fresh weight and dry weight, the leaf soluble protein content were lower, and the leaf POD activity, the MDA content were higher than those under well watering.”
t0“Brassica napus L.”
Claim · model-written summary
Waterlogging made leaf soluble protein content lower than well watering.
- Taxon
- Brassica napus L.Brassica napust0
- Treatment
- waterlogginge0
- Outcome
- leaf soluble protein contente0
- Direction
- lowere0
- Comparator
- well wateringe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under waterlogging, the number of green leaf, the shoot fresh weight and dry weight, the leaf soluble protein content were lower, and the leaf POD activity, the MDA content were higher than those under well watering.”
t0“Brassica napus L.”
Claim · model-written summary
Waterlogging made leaf POD activity higher than well watering.
- Taxon
- Brassica napus L.Brassica napust0
- Treatment
- waterlogginge0
- Outcome
- leaf POD activitye0
- Direction
- highere0
- Comparator
- well wateringe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under waterlogging, the number of green leaf, the shoot fresh weight and dry weight, the leaf soluble protein content were lower, and the leaf POD activity, the MDA content were higher than those under well watering.”
t0“Brassica napus L.”
Claim · model-written summary
Waterlogging made MDA content higher than well watering.
- Taxon
- Brassica napus L.Brassica napust0
- Treatment
- waterlogginge0
- Outcome
- MDA contente0
- Direction
- highere0
- Comparator
- well wateringe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under waterlogging, the number of green leaf, the shoot fresh weight and dry weight, the leaf soluble protein content were lower, and the leaf POD activity, the MDA content were higher than those under well watering.”
t0“Brassica napus L.”
Claim · model-written summary
Root fresh and dry weights were lower in most accessions under waterlogging.
- Taxon
- Brassica napus L.Brassica napust0
- Treatment
- waterlogging stresse0
- Outcome
- root fresh weight and dry weighte0
- Direction
- lowere0
- Qualifier
- most accessionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The root fresh weight and dry weight were lower in most accessions and higher in some accessions under waterlogging stress.”
t0“Brassica napus L.”
Claim · model-written summary
Root fresh and dry weights were higher in some accessions under waterlogging.
- Taxon
- Brassica napus L.Brassica napust0
- Treatment
- waterlogging stresse0
- Outcome
- root fresh weight and dry weighte0
- Direction
- highere0
- Qualifier
- some accessionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The root fresh weight and dry weight were lower in most accessions and higher in some accessions under waterlogging stress.”
t0“Brassica napus L.”
Claim · model-written summary
GWAS identified 36 SNPs associated with waterlogging traits.
- Taxon
- Brassica napus L.Brassica napust0
- Gene
- SNPse0
- Outcome
- SNPs were identified associated with waterlogging traitse0
- Value
- 36e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 36 SNPs were identified associated with waterlogging traits and explained the phenotypic variation of 8.28%-12.95%”
t0“Brassica napus L.”
Claim · model-written summary
The SNPs explained 8.28%-12.95% of waterlogging trait variation.
- Taxon
- Brassica napus L.Brassica napust0
- Gene
- SNPse0
- Outcome
- phenotypic variatione0
- Value
- 8.28%-12.95%e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“36 SNPs were identified associated with waterlogging traits and explained the phenotypic variation of 8.28%-12.95%”
t0“Brassica napus L.”
Claim · model-written summary
Waterlogging resistance index differed among genotypes at 17 SNPs.
- Taxon
- Brassica napus L.Brassica napust0
- Gene
- SNPse0
- Outcome
- waterlogging resistance indexe0
- Value
- 17 SNPse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There were significant (P < 0.05) or extremely significant (P < 0.01) differences in the waterlogging resistance index among genotypes of 17 SNPs”
t0“Brassica napus L.”
Claim · model-written summary
Blocks with 17 SNPs covered 71 candidate genes.
- Taxon
- Brassica napus L.Brassica napust0
- Outcome
- candidate genese0
- Value
- 71e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Blocks containing 17 SNPs covered 71 candidate genes”
t0“Brassica napus L.”
Claim · model-written summary
Among the candidate genes, 64 were homologous to Arabidopsis genes.
- Taxon
- Brassica napus L.Brassica napust0
- Outcome
- candidate genes were homologous to Arabidopsis genese0
- Value
- 64e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among them, 64 candidate genes were homologous to Arabidopsis genes by blast”
t0“Brassica napus L.”
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