Abstract
The specific partnership between legumes and rhizobia relies on a chemical dialogue. Plant flavonoids activate the bacterial transcription factor NodD, which triggers production of Nod factors that are recognized by the plant. Structural studies of the Pisum sativum (pea) symbiont Rhizobium leguminosarum NodD revealed two pockets that are essential for its activation by flavonoids. Comparative studies with NodD1 of Sinorhizobium medicae , the symbiont of Medicago truncatula , revealed that this specificity is determined by the shape of the pocket and by specific amino acids. A chimeric NodD containing the flavonoid recognition residues from S. medicae NodD1 in the R. leguminosarum NodD backbone was sufficient to complement nitrogen fixation in M. truncatula by an S. medicae nodD1 mutant, confirming the critical role of flavonoid recognition in host range.
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Claims 3
Claim · model-written summary
Rhizobium leguminosarum NodD has two pockets essential for activation by flavonoids.
- Taxon
- Pisum sativum
Lathyrus oleraceuse0 - Interactor
- Rhizobium leguminosarum
Rhizobium leguminosarume0symbiont - Gene
- Rhizobium leguminosarum NodDe0
- Outcome
- two pockets that are essential for its activation by flavonoidse0
- Value
- two pocketse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Structural studies of the Pisum sativum (pea) symbiont Rhizobium leguminosarum NodD revealed two pockets that are essential for its activation by flavonoids.”
Claim · model-written summary
NodD specificity was determined by pocket shape and specific amino acids.
- Taxon
- Medicago truncatula
Medicago truncatulae0 - Interactor
- Sinorhizobium medicae
Sinorhizobium medicaee0symbiont - Gene
- NodD1e0
- Outcome
- this specificitye0
- Qualifier
- shape of the pocket and by specific amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Comparative studies with NodD1 of Sinorhizobium medicae , the symbiont of Medicago truncatula , revealed that this specificity is determined by the shape of the pocket and by specific amino acids.”
Claim · model-written summary
A chimeric NodD complemented nitrogen fixation in Medicago truncatula by an S. medicae nodD1 mutant.
- Taxon
- M. truncatulaMedicago truncatulae0
- Treatment
- A chimeric NodD containing the flavonoid recognition residues from S. medicae NodD1 in the R. leguminosarum NodD backbonee0
- Interactor
- S. medicaeSinorhizobium medicaee0symbiont
- Gene
- chimeric NodDe0
- Outcome
- nitrogen fixation in M. truncatulae0
- Direction
- complemente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A chimeric NodD containing the flavonoid recognition residues from S. medicae NodD1 in the R. leguminosarum NodD backbone was sufficient to complement nitrogen fixation in M. truncatula by an S. medicae nodD1 mutant”