Abstract
Achnatherum inebrians is a perennial plant belonging to the Poaceae, subfamily Pooideae, which often forms a symbiosis with fungal endophytes of the genus Epichloë. In this study, E. gansuensis-infected (E+) and E. gansuensis-free (E−) A. inebrians were inoculated with Blumeria graminis (pathogen-inoculated, P+; non-inoculated, P−) under greenhouse conditions. Gas chromatography-mass spectrometry (GC-MS) and transcriptome sequencing were combined to characterize the response of the leaf cuticular wax of A. inebrians plants with and without the Epichloë endophyte to B. graminis stress. The present results revealed that the predominant components of the cuticular wax of A. inebrians leaves under the Epichloë endophyte and B. graminis treatments were hydrocarbons, esters, and fatty acids. The total cuticular wax and ester contents of the E+ and E− A. inebrians leaves significantly (p < 0.05) increased under the B. graminis treatment, but the total cuticular wax and ester contents of the E+ leaves were greater than those of the E− plants. Through transcriptome analysis, 15 genes that were differentially expressed and related to cuticular wax biosynthesis were identified, including fadD, fadF, TER, SSI2, BiP, CER1, FAR, and KCS.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
Total cuticular wax and ester contents of E+ Achnatherum inebrians leaves significantly increased under Blumeria graminis treatment compared with non-inoculated plants.
- Taxon
- Achnatherum inebriansAchnatherum inebrianst0
- Material
- E+e1
- Treatment
- B. graminis treatmente1
- Interactor
- Blumeria graminis
Blumeria graminise0pathogen - Second interactor
- E. gansuensise0symbiont
- Outcome
- total cuticular wax and ester contentse1
- Direction
- increasede1
- Qualifier
- p < 0.05e1
- Comparator
- non-inoculatede0
- Setting
- greenhouse conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Achnatherum inebrians under Blumeria graminis infection”
e0“E. gansuensis-infected (E+) and E. gansuensis-free (E−) A. inebrians were inoculated with Blumeria graminis (pathogen-inoculated, P+; non-inoculated, P−) under greenhouse conditions”
e1“The total cuticular wax and ester contents of the E+ and E− A. inebrians leaves significantly (p < 0.05) increased under the B. graminis treatment”
Claim · model-written summary
Total cuticular wax and ester contents of E− Achnatherum inebrians leaves significantly increased under Blumeria graminis treatment compared with non-inoculated plants.
- Taxon
- Achnatherum inebriansAchnatherum inebrianst0
- Material
- E−e1
- Treatment
- B. graminis treatmente1
- Interactor
- Blumeria graminisBlumeria graminise0pathogen
- Second interactor
- E. gansuensise0symbiont
- Outcome
- total cuticular wax and ester contentse1
- Direction
- increasede1
- Qualifier
- p < 0.05e1
- Comparator
- non-inoculatede0
- Setting
- greenhouse conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Achnatherum inebrians under Blumeria graminis infection”
e0“E. gansuensis-infected (E+) and E. gansuensis-free (E−) A. inebrians were inoculated with Blumeria graminis (pathogen-inoculated, P+; non-inoculated, P−) under greenhouse conditions”
e1“The total cuticular wax and ester contents of the E+ and E− A. inebrians leaves significantly (p < 0.05) increased under the B. graminis treatment”
Claim · model-written summary
Total cuticular wax and ester contents were greater in E+ than in E− Achnatherum inebrians leaves.
Verbatim
t0“in Achnatherum inebrians under Blumeria graminis infection”
e2“the total cuticular wax and ester contents of the E+ leaves were greater than those of the E− plants”
Claim · model-written summary
The predominant cuticular wax components of Achnatherum inebrians leaves under the Epichloe endophyte and B. graminis treatments were hydrocarbons, esters and fatty acids.
- Taxon
- Achnatherum inebriansAchnatherum inebrianst0
- Organ / stage
- leavese3
- Treatment
- Epichloë endophyte and B. graminis treatmentse3
- Interactor
- B. graminisBlumeria graminise3pathogen
- Second interactor
- Epichloë endophytee3symbiont
- Outcome
- hydrocarbons, esters, and fatty acidse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Achnatherum inebrians under Blumeria graminis infection”
e3“the predominant components of the cuticular wax of A. inebrians leaves under the Epichloë endophyte and B. graminis treatments were hydrocarbons, esters, and fatty acids”
Claim · model-written summary
Transcriptome analysis identified 15 differentially expressed genes related to cuticular wax biosynthesis in Achnatherum inebrians, including fadD, fadF, TER, SSI2, BiP, CER1, FAR and KCS.
- Taxon
- Achnatherum inebriansAchnatherum inebrianst0
- Gene
- fadD, fadF, TER, SSI2, BiP, CER1, FAR, and KCSe4
- Outcome
- genes that were differentially expressed and related to cuticular wax biosynthesise4
- Value
- 15e4
Verbatim
t0“in Achnatherum inebrians under Blumeria graminis infection”
e4“Through transcriptome analysis, 15 genes that were differentially expressed and related to cuticular wax biosynthesis were identified, including fadD, fadF, TER, SSI2, BiP, CER1, FAR, and KCS”