Abstract
Bacillus velezensis Jin12 with great starch degradation ability was isolated from stored tobacco lamina. In this study, PacBio sequencing technique was employed to analyze the genome of strain Jin12. The results revealed that the strain Jin12 genome is 4,057,213 bp in size, with a GC content of 46.33%, and encodes a total of 4,165 genes. GO annotation showed that the genes in strain Jin12 were enriched in the terms: catalytic activity, binding and transporter activity. COG annotation demonstrated that most genes in strain Jin12 were involved in amino acid transport and metabolism, transcription, and carbohydrate transport and metabolism. Sequence similarity searches using the CAZyme database helped identify the enzyme repertoire of strain Jin12 involved in starch degradation. We identified four α-amylases in the genome of strain Jin12 that likely participated in starch degradation. The experiment involving spray application of strain Jin12 bacterial suspension demonstrated that strain Jin12 degraded starch in tobacco leaves from different geographical origins, confirming its role in starch degradation. The current study enhances our understanding of the starch degradation mechanism from a genetic perspective and facilitates the application of starch-degrading strains in improving tobacco quality.
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Claims 7
Claim · model-written summary
The Jin12 genome is 4,057,213 bp in size
- Taxon
- tobacco
Nicotiana tabacumt0 - Outcome
- genomee0
- Value
- 4,057,213 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“flue-cured tobacco leaves”
e0“The results revealed that the strain Jin12 genome is 4,057,213 bp in size, with a GC content of 46.33%”
Claim · model-written summary
The Jin12 genome has a GC content of 46.33%
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- GC contente0
- Value
- 46.33%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“flue-cured tobacco leaves”
e0“The results revealed that the strain Jin12 genome is 4,057,213 bp in size, with a GC content of 46.33%”
Claim · model-written summary
The Jin12 genome encodes 4,165 genes
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- genese1
- Value
- 4,165e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“flue-cured tobacco leaves”
e1“encodes a total of 4,165 genes”
Claim · model-written summary
Genes of the Jin12 genome are enriched in catalytic activity, binding and transporter activity terms
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- genese2
- Qualifier
- catalytic activity, binding and transporter activitye2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“flue-cured tobacco leaves”
e2“GO annotation showed that the genes in strain Jin12 were enriched in the terms: catalytic activity, binding and transporter activity”
Claim · model-written summary
Most Jin12 genes are involved in amino acid transport and metabolism, transcription, and carbohydrate transport and metabolism
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- genese3
- Qualifier
- amino acid transport and metabolism, transcription, and carbohydrate transport and metabolisme3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“flue-cured tobacco leaves”
e3“COG annotation demonstrated that most genes in strain Jin12 were involved in amino acid transport and metabolism, transcription, and carbohydrate transport and metabolism”
Claim · model-written summary
Four amylase genes in the Jin12 genome likely participate in starch degradation
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- starch degradatione5
- Value
- foure4
- Qualifier
- likely participatede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“flue-cured tobacco leaves”
e4“We identified four”
e5“that likely participated in starch degradation”
Claim · model-written summary
Spray application of Jin12 bacterial suspension degraded starch in tobacco leaves from different geographical origins
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- leavese6
- Treatment
- spray application of strain Jin12 bacterial suspensione6
- Interactor
- strain Jin12e6other
- Outcome
- starche6
- Direction
- degradede6
- Setting
- different geographical originse6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“flue-cured tobacco leaves”
e6“The experiment involving spray application of strain Jin12 bacterial suspension demonstrated that strain Jin12 degraded starch in tobacco leaves from different geographical origins”
The same questions in other papers
application · quality: sugar starch · Nicotiana tabacum (tobacco)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.