Paper · 2026 · Food Chemistry X

Comprehensive volatile profiling and metabolomics analysis of cowpea

Xiuhua He; Yanchi Zhou; Qiaozhu Wei; et al.

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citations
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Open access · cc-by-nc-nd

Abstract

L. Walp., Vu) varieties remains poorly understood, limiting targeted quality improvement. This study integrated headspace gas chromatography-ion mobility spectrometry with ultra-high-performance liquid chromatography-tandem mass spectrometry to comprehensively analyze metabolites in 14 Chinese cowpea varieties. A total of 174 volatile organic compounds were identified, with esters, heterocyclic compounds, and ketones accounting for 51.15% of the total. Principal component analysis and fingerprint clustering revealed variety-specific distribution patterns of volatile components, with the Vu-1 variety showing significant enrichment in β-pinene and 3-pentanol. Metabolomic analysis further identified 392 differentially expressed metabolites, revealing that amino acid metabolism and flavonoid metabolism are the core pathways determining varietal differences. This study systematically elucidates the chemical characteristics of cowpea varieties from a metabolomic perspective, providing important evidence for quality improvement and high-value utilization of legume crops.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 6

Observationobservedoriginal resultmeasured

Claim · model-written summary

The 14 cowpea varieties contained 174 identified volatile organic compounds.

Outcome
volatile organic compoundse0
Value
174e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“A total of 174 volatile organic compounds were identified”

✓ located verbatim in the source · abstract, characters 313–370 · show in text ↑
t0

“cowpea”

✓ located verbatim in the source · title, characters 62–68 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Esters, heterocyclic compounds and ketones accounted for 51.15% of the volatile compounds.

Outcome
esters, heterocyclic compounds, and ketonese0
Value
51.15%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“esters, heterocyclic compounds, and ketones accounting for 51.15% of the total”

✓ located verbatim in the source · abstract, characters 377–455 · show in text ↑
t0

“cowpea”

✓ located verbatim in the source · title, characters 62–68 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Volatile distributions were variety specific.

Outcome
variety-specific distribution patterns of volatile componentse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Principal component analysis and fingerprint clustering revealed variety-specific distribution patterns of volatile components”

✓ located verbatim in the source · abstract, characters 457–583 · show in text ↑
t0

“cowpea”

✓ located verbatim in the source · title, characters 62–68 · show in text ↑
Observationobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Vu-1 was enriched in β-pinene and 3-pentanol.

Material
Vu-1e0
Outcome
enrichment in β-pinene and 3-pentanole0
Direction
enrichmente0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Vu-1 variety showing significant enrichment in β-pinene and 3-pentanol”

✓ located verbatim in the source · abstract, characters 594–664 · show in text ↑
t0

“cowpea”

✓ located verbatim in the source · title, characters 62–68 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Metabolomics identified 392 differentially expressed metabolites.

Outcome
differentially expressed metabolitese0
Value
392e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“identified 392 differentially expressed metabolites”

✓ located verbatim in the source · abstract, characters 695–746 · show in text ↑
t0

“cowpea”

✓ located verbatim in the source · title, characters 62–68 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

Amino acid and flavonoid metabolism were identified as core pathways of varietal differences.

Outcome
varietal differencese0
Qualifier
amino acid metabolism and flavonoid metabolisme0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“amino acid metabolism and flavonoid metabolism are the core pathways determining varietal differences”

✓ located verbatim in the source · abstract, characters 763–864 · show in text ↑
t0

“cowpea”

✓ located verbatim in the source · title, characters 62–68 · show in text ↑