Abstract
Abstract High-temperature (HT) stress poses a major threat to plant growth and physiological functions by disrupting cellular homeostasis and metabolic processes. Despite extensive studies, the molecular and physiological mechanisms underlying plant adaptation to HT stress remain incompletely understood. This study investigates the role of cysteine (CYS), a thiol-containing amino acid, in enhancing high-temperature tolerance in Arabidopsis thaliana ( A. thaliana ) through the regulation of heat shock protein 90 (HSP90) and the glyoxalase (GLX) system. Our research demonstrates that CYS treatment under HT stress significantly enhances key physiological parameters, including relative water content (RWC), and total chlorophyll levels while reducing oxidative damage markers like thiobarbituric acid reactive substances (TBARS), and hydrogen peroxide (H₂O₂). In this study, findings from A. thaliana (Col-0, hsp90.1 and hsp90.4 mutants, and those subjected to Glyoxalase I inhibitor (S-p-bromobenzylglutathione cyclopentyl diester (BBGD) treatment) reveal that CYS acts as a positive regulator of the GLX system by boosting the activities of Glyoxalase I (GLXI) and Glyoxalase II (GLXII) enzymes involved in methylglyoxal (MG) detoxification, particularly in conjunction with HSP90.1 and HSP90.4. The effects of GLXI inhibitor on the GLX system were experimentally studied for the first time on plants by applying to A. thaliana seedlings (Col-0 and hsp90.4 mutant). Moreover, CYS treatment enhances the expression of genes related to the GLX system and HSPs, leading to improved thermotolerance in A. thaliana . In conclusion, our findings highlight a synergistic interaction between CYS, the GLX system, and HSP90 proteins, suggesting promising genetic and chemical approaches for enhancing plant tolerance to high-temperature stress.
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Claims 5
Claim · model-written summary
Cysteine under heat stress significantly raised relative water content and total chlorophyll in Arabidopsis.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Treatment
- CYS treatment under HT stresse0
- Outcome
- relative water content (RWC), and total chlorophyll levelse0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CYS treatment under HT stress significantly enhances key physiological parameters, including relative water content (RWC), and total chlorophyll levels”
t0“Arabidopsis thaliana”
Claim · model-written summary
Cysteine under heat stress reduced TBARS and hydrogen peroxide.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- CYS treatment under HT stresse0
- Outcome
- thiobarbituric acid reactive substances (TBARS), and hydrogen peroxide (H₂O₂)e0
- Direction
- reducinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CYS treatment under HT stress significantly enhances key physiological parameters, including relative water content (RWC), and total chlorophyll levels while reducing oxidative damage markers like thiobarbituric acid reactive substances (TBARS), and hydrogen peroxide (H₂O₂)”
t0“Arabidopsis thaliana”
Claim · model-written summary
Cysteine boosted glyoxalase I and II activities in Arabidopsis, particularly with HSP90.1 and HSP90.4.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- CYSe0
- Gene
- HSP90.1 and HSP90.4e0
- Outcome
- activities of Glyoxalase I (GLXI) and Glyoxalase II (GLXII)e0
- Direction
- boostinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CYS acts as a positive regulator of the GLX system by boosting the activities of Glyoxalase I (GLXI) and Glyoxalase II (GLXII) enzymes involved in methylglyoxal (MG) detoxification, particularly in conjunction with HSP90.1 and HSP90.4”
t0“Arabidopsis thaliana”
Claim · model-written summary
Cysteine enhanced expression of GLX system and HSP genes.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- CYS treatmente0
- Outcome
- expression of genes related to the GLX system and HSPse0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CYS treatment enhances the expression of genes related to the GLX system and HSPs”
t0“Arabidopsis thaliana”
Claim · model-written summary
Cysteine treatment improved thermotolerance in Arabidopsis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- CYS treatmente0
- Outcome
- thermotolerancee0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CYS treatment enhances the expression of genes related to the GLX system and HSPs, leading to improved thermotolerance in A. thaliana”
t0“Arabidopsis thaliana”
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cysteine · tolerance: abiotic · Arabidopsis thaliana (thale cress)Results agree
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- negative
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.