Paper · 2026 · Plant Disease

The potential of ozone nanobubbles in suppressing cucumber powdery mildew

Ewumi Azeez Folorunso; Radek Gebauer; Jan Mraz; et al.

6
claims
11
verbatim quotes
0
citations
2
research areas
—
Not open access

Abstract

This study evaluated the efficacy of ozone nanobubbles (O 3 -NB) as a foliar treatment to suppress cucumber powdery mildew was evaluated in a controlled bioassay under optimal and suboptimal relative humidity (RH) conditions. O 3 -NB solutions were generated at three concentrations (0.54 ± 0.08, 1.16 ± 0.23, and 2.08 ± 0.22 mg L -1 ) and compared with oxygen nanobubbles (O 2 -NB) and distilled water controls. In a 20-day growth chamber bioassay under three relative humidity conditions (≤40 %, 65-73 %, and ≥95 % RH), leaves treated with 0.5 mgL -1 O 3 -NB exhibited suppression of powdery mildew by approximately 20 ± 3.2 % and 33 ± 4.6 % compared to the control at moderate and low humidity. However, efficacy declined under high relative humidity (≥95 % RH). A 16-week hydroponic study confirmed that foliar application of O 3 -NB at 0.5-2 mg L -1 caused no adverse effects on cucumber growth, yield, or physical appearance. Although, efficacy was lower than typically reported for conventional fungicides, O 3 -NB offers a potentially safer, residue-free alternative for integrated pest management programs, particularly during withholding periods where chemical fungicides cannot be used.

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Claims 6

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Leaves treated with 0.5 mg/L O3-NB showed about 20 ± 3.2% suppression of cucumber powdery mildew compared with control at 65-73% relative humidity.

Taxon
cucumberCucumis sativuse0
Treatment
0.5 mgL -1 O 3 -NBe1
Interactor
powdery mildewe0pathogen
Outcome
powdery mildewe1
Direction
suppressione1
Value
20 ± 3.2 %e1
Comparator
controle1
Setting
65-73 %e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“This study evaluated the efficacy of ozone nanobubbles (O 3 -NB) as a foliar treatment to suppress cucumber powdery mildew was evaluated in a controlled bioassay under optimal and suboptimal relative humidity (RH) conditions”

✓ located verbatim in the source · abstract, characters 0–224 · show in text ↑
e1

“In a 20-day growth chamber bioassay under three relative humidity conditions (≤40 %, 65-73 %, and ≥95 % RH), leaves treated with 0.5 mgL -1 O 3 -NB exhibited suppression of powdery mildew by approximately 20 ± 3.2 % and 33 ± 4.6 % compared to the control at moderate and low humidity”

✓ located verbatim in the source · abstract, characters 413–696 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Leaves treated with 0.5 mg/L O3-NB showed about 33 ± 4.6% suppression of cucumber powdery mildew compared with control at ≤40% relative humidity.

Taxon
cucumberCucumis sativuse0
Treatment
0.5 mgL -1 O 3 -NBe1
Interactor
powdery mildewe0pathogen
Outcome
powdery mildewe1
Direction
suppressione1
Value
33 ± 4.6 %e1
Comparator
controle1
Setting
≤40 %e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“This study evaluated the efficacy of ozone nanobubbles (O 3 -NB) as a foliar treatment to suppress cucumber powdery mildew was evaluated in a controlled bioassay under optimal and suboptimal relative humidity (RH) conditions”

✓ located verbatim in the source · abstract, characters 0–224 · show in text ↑
e1

“In a 20-day growth chamber bioassay under three relative humidity conditions (≤40 %, 65-73 %, and ≥95 % RH), leaves treated with 0.5 mgL -1 O 3 -NB exhibited suppression of powdery mildew by approximately 20 ± 3.2 % and 33 ± 4.6 % compared to the control at moderate and low humidity”

✓ located verbatim in the source · abstract, characters 413–696 · show in text ↑
Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

Efficacy of ozone nanobubbles against cucumber powdery mildew declined under high relative humidity (≥95% RH).

Taxon
cucumberCucumis sativuse0
Outcome
efficacye2
Direction
declinede2
Setting
high relative humidity (≥95 % RH)e2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“This study evaluated the efficacy of ozone nanobubbles (O 3 -NB) as a foliar treatment to suppress cucumber powdery mildew was evaluated in a controlled bioassay under optimal and suboptimal relative humidity (RH) conditions”

✓ located verbatim in the source · abstract, characters 0–224 · show in text ↑
e2

“However, efficacy declined under high relative humidity (≥95 % RH)”

✓ located verbatim in the source · abstract, characters 698–764 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Foliar application of O3-NB at 0.5-2 mg/L caused no adverse effects on cucumber growth, yield or appearance in a 16-week hydroponic study.

Taxon
cucumberCucumis sativuse3
Treatment
foliar application of O 3 -NB at 0.5-2 mg L -1e3
Outcome
growth, yield, or physical appearancee3
Direction
no adverse effectse3
Setting
hydroponice3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e3

“A 16-week hydroponic study confirmed that foliar application of O 3 -NB at 0.5-2 mg L -1 caused no adverse effects on cucumber growth, yield, or physical appearance”

✓ located verbatim in the source · abstract, characters 766–930 · show in text ↑
Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

Efficacy of ozone nanobubbles against powdery mildew was lower than typically reported for conventional fungicides.

Taxon
cucumberCucumis sativuse0
Outcome
efficacye4
Direction
lowere4
Comparator
conventional fungicidese4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“This study evaluated the efficacy of ozone nanobubbles (O 3 -NB) as a foliar treatment to suppress cucumber powdery mildew was evaluated in a controlled bioassay under optimal and suboptimal relative humidity (RH) conditions”

✓ located verbatim in the source · abstract, characters 0–224 · show in text ↑
e4

“Although, efficacy was lower than typically reported for conventional fungicides, O 3 -NB offers a potentially safer, residue-free alternative for integrated pest management programs, particularly during withholding periods where chemical fungicides cannot be used”

✓ located verbatim in the source · abstract, characters 932–1196 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Ozone nanobubbles are suggested as a residue-free alternative for integrated pest management in cucumber.

Taxon
cucumberCucumis sativuse0
Outcome
residue-free alternative for integrated pest management programse4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“This study evaluated the efficacy of ozone nanobubbles (O 3 -NB) as a foliar treatment to suppress cucumber powdery mildew was evaluated in a controlled bioassay under optimal and suboptimal relative humidity (RH) conditions”

✓ located verbatim in the source · abstract, characters 0–224 · show in text ↑
e4

“Although, efficacy was lower than typically reported for conventional fungicides, O 3 -NB offers a potentially safer, residue-free alternative for integrated pest management programs, particularly during withholding periods where chemical fungicides cannot be used”

✓ located verbatim in the source · abstract, characters 932–1196 · show in text ↑

ozone · yield · Cucumis sativus (cucumber)Positive and negative results

4 papers: 3 positive, 1 negative or no effect

3 in the opposite direction

  1. positive
  2. positive
  3. positive
See all 4 papers →

application · yield · Cucumis sativus (cucumber)Positive and negative results

73 papers: 65 positive, 8 negative or no effect

7 papers in the same direction as this paper · 65 in the opposite direction

  1. positive
  2. positive
  3. positive

69 papers more in this group

See all 73 papers →

ozone · disease · Cucumis sativus (cucumber)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.