Paper · 2026 · Agriculture

Evaluating Wheat Seed Quality: Performance, Stability, and Genetic Control Across Six Greek Environments Using Multiple Selection Designs

Vasileios Greveniotis; Elisavet Bouloumpasi; Adriana Skendi; et al.

14
claims
31
verbatim quotes
2
citations
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research areas
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Open access · cc-by

Abstract

Wheat seed quality is a key factor of end-use performance and nutritional value, yet it is strongly influenced by both genetic and environmental factors. The present study evaluated the performance, stability, and genetic control of wheat seed quality traits across six contrasting environments in Greece, focusing on genotypes derived from three selection designs (McGinnis & Shebeski, honeycomb, and gridding) and a local landrace. The measured traits included crude protein, fat, ash, starch, crude fibre, Zeleny sedimentation, carbohydrate, soluble fraction, non-starch fraction, and moisture. A combined ANOVA revealed significant effects of genotype, environment, and their interaction on all traits. Crude protein, fat, ash, and carbohydrate were predominantly governed by genotype, while starch, Zeleny sedimentation, soluble fraction, non-starch fraction, and moisture were more influenced by environmental factors, while crude fiber showed balanced genotype × environment effects. Stability analysis identified genotypes with consistent expression of key quality traits across environments, demonstrating the relevance of stability parameters for reliable selection. Correlation analysis indicated positive associations among protein, fat, Zeleny sedimentation, and crude fiber, and negative relationships with starch, carbohydrate, soluble fraction, and non-starch fraction, revealing trade-offs among wheat seed quality components. Selection method influenced trait expression, with gridding-derived lines excelling in protein and fat, McGinnis & Shebeski lines in Zeleny sedimentation and fiber, and honeycomb-derived lines in starch, carbohydrate, soluble, and non-starch fractions. Overall, the results support the use of multi-environment evaluation and stability-based selection to improve wheat seed quality in a predictable and targeted manner.

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Claims 14

Associationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Genotype, environment and their interaction significantly affected all measured seed quality traits.

Outcome
all traitse0
Setting
environmente0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“A combined ANOVA revealed significant effects of genotype, environment, and their interaction on all traits.”

✓ located verbatim in the source · abstract, characters 598–706 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 11–16 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Crude protein, fat, ash and carbohydrate were predominantly governed by genotype.

Material
genotypee0
Outcome
Crude protein, fat, ash, and carbohydratee0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Crude protein, fat, ash, and carbohydrate were predominantly governed by genotype”

✓ located verbatim in the source · abstract, characters 707–788 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 11–16 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Starch, Zeleny sedimentation and soluble fraction were more influenced by environmental factors.

Outcome
starch, Zeleny sedimentation, soluble fractione0
Direction
more influencede0
Setting
environmental factorse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“starch, Zeleny sedimentation, soluble fraction, non-starch fraction, and moisture were more influenced by environmental factors”

✓ located verbatim in the source · abstract, characters 796–923 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 11–16 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Non-starch fraction and moisture were more influenced by environmental factors.

Outcome
non-starch fraction, and moisturee0
Direction
more influencede0
Setting
environmental factorse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“non-starch fraction, and moisture were more influenced by environmental factors”

✓ located verbatim in the source · abstract, characters 844–923 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 11–16 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Crude fiber showed balanced genotype by environment effects.

Outcome
crude fibere0
Setting
environmente0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“crude fiber showed balanced genotype × environment effects”

✓ located verbatim in the source · abstract, characters 931–989 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 11–16 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Some genotypes showed consistent expression of key quality traits across environments.

Material
genotypese0
Outcome
consistent expression of key quality traitse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Stability analysis identified genotypes with consistent expression of key quality traits across environments”

✓ located verbatim in the source · abstract, characters 991–1099 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 11–16 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Protein, fat, Zeleny sedimentation and crude fiber had positive associations.

Outcome
protein, fat, Zeleny sedimentation, and crude fibere0
Direction
positive associationse0

Not reportedOrgan / stage

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“positive associations among protein, fat, Zeleny sedimentation, and crude fiber”

✓ located verbatim in the source · abstract, characters 1208–1287 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 11–16 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Starch, carbohydrate and soluble fraction showed negative relationships with the positively associated quality traits.

Outcome
starch, carbohydrate, soluble fractione0
Direction
negative relationshipse0
Comparator
protein, fat, Zeleny sedimentation, and crude fibere1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“negative relationships with starch, carbohydrate, soluble fraction, and non-starch fraction”

✓ located verbatim in the source · abstract, characters 1293–1384 · show in text ↑
e1

“positive associations among protein, fat, Zeleny sedimentation, and crude fiber”

✓ located verbatim in the source · abstract, characters 1208–1287 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 11–16 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Non-starch fraction showed negative relationships with the positively associated quality traits.

Outcome
non-starch fractione0
Direction
negative relationshipse0
Comparator
protein, fat, Zeleny sedimentation, and crude fibere1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“negative relationships with starch, carbohydrate, soluble fraction, and non-starch fraction”

✓ located verbatim in the source · abstract, characters 1293–1384 · show in text ↑
e1

“positive associations among protein, fat, Zeleny sedimentation, and crude fiber”

✓ located verbatim in the source · abstract, characters 1208–1287 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 11–16 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Gridding-derived lines excelled in protein and fat.

Material
gridding-derived linese0
Outcome
protein and fate0
Direction
excellinge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“gridding-derived lines excelling in protein and fat”

✓ located verbatim in the source · abstract, characters 1495–1546 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 11–16 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

McGinnis & Shebeski lines excelled in Zeleny sedimentation and fiber.

Material
McGinnis & Shebeski linese0
Outcome
Zeleny sedimentation and fibere0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“McGinnis & Shebeski lines in Zeleny sedimentation and fiber”

✓ located verbatim in the source · abstract, characters 1548–1607 · show in text ↑
e1

“excelling in protein and fat”

✓ located verbatim in the source · abstract, characters 1518–1546 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 11–16 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Honeycomb-derived lines excelled in starch, carbohydrate and soluble fractions.

Material
honeycomb-derived linese0
Outcome
starch, carbohydrate, solublee0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“honeycomb-derived lines in starch, carbohydrate, soluble, and non-starch fractions”

✓ located verbatim in the source · abstract, characters 1613–1695 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 11–16 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Honeycomb-derived lines excelled in non-starch fractions.

Material
honeycomb-derived linese0
Outcome
non-starch fractionse0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“honeycomb-derived lines in starch, carbohydrate, soluble, and non-starch fractions”

✓ located verbatim in the source · abstract, characters 1613–1695 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 11–16 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors support multi-environment evaluation and stability-based selection to improve wheat seed quality.

Outcome
multi-environment evaluation and stability-based selectione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the results support the use of multi-environment evaluation and stability-based selection to improve wheat seed quality in a predictable and targeted manner”

✓ located verbatim in the source · abstract, characters 1706–1862 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 11–16 · show in text ↑