Paper · 2026 · Nutrient Cycling in Agroecosystems

Herbage nitrogen uptake in grass-only and grass-white clover swards receiving zero nitrogen

Murray Áine; Delaby Luc; Burchill William; et al.

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verbatim quotes
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citations
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Open access · cc-by-nc-nd

Abstract

Nitrogen (N) fertilisers are major contributors to ammonia and greenhouse gas emissions through their inputs in grazed herbage systems. There is significant need to improve our understanding of more sustainable N inputs such as soil organic N mineralisation and biological N fixation to reduce our reliance on chemical N fertiliser. The objective of this study was to evaluate the herbage N uptake from grass-only and grass-white clover (Trifolium repens L.) herbage receiving zero external N inputs across two contrasting soils and climatic conditions in temperate pasture-based systems. The study consisted of zero N plots established in 2020 and 2021 within grazed paddocks of either grass-only or grass-white clover swards at two sites. The plots received no chemical or organic N and were not grazed for the duration of the study. Plots were yielded simultaneously as the paddocks were rotationally grazed by dairy cows. Nitrogen content was measured by Leco and N yield was calculated by multiplying herbage yield by N content. The grass-only sward plots yielded 7,682 kg DM/ha per year and 185 kg N/ha per year across the 2 years and sites. The grass-white clover swards yielded significantly higher herbage (10,166 kg DM/ha per year) and N (284 kg N/ha per year) than the grass-only swards (P < 0.001). The results provide an indication of background N mineralisation of 185 kg N/ha from the organic matter content of the soil and biological N fixation of 99 kg N/ha from the white clover within grass-only and grass-clover swards receiving zero N.

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Claims 6

Observationobservedoriginal resultmeasured

Claim · model-written summary

Grass-only swards yielded 7,682 kg DM/ha per year.

Taxon
grasse0
Outcome
yieldede0
Value
7,682 kg DM/ha per yeare0

Verbatim

e0

“The grass-only sward plots yielded 7,682 kg DM/ha per year and 185 kg N/ha per year across the 2 years and sites”

✓ located verbatim in the source · abstract, characters 1034–1146 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Grass-only swards yielded 185 kg N/ha per year.

Taxon
grasse0
Outcome
yieldede0
Value
185 kg N/ha per yeare0

Verbatim

e0

“The grass-only sward plots yielded 7,682 kg DM/ha per year and 185 kg N/ha per year across the 2 years and sites”

✓ located verbatim in the source · abstract, characters 1034–1146 · show in text ↑
Associationobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Grass-white clover swards yielded significantly higher herbage (10,166 kg DM/ha per year) than grass-only swards.

Taxon
grass-white clovere0
Outcome
herbagee0
Direction
highere0
Value
10,166 kg DM/ha per yeare0
Qualifier
P < 0.001e0
Comparator
grass-only swardse0

Verbatim

e0

“The grass-white clover swards yielded significantly higher herbage (10,166 kg DM/ha per year) and N (284 kg N/ha per year) than the grass-only swards (P < 0.001)”

✓ located verbatim in the source · abstract, characters 1148–1309 · show in text ↑
Associationobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Grass-white clover swards took up significantly more N (284 kg N/ha per year) than grass-only swards.

Taxon
grass-white clovere0
Outcome
Ne0
Direction
highere0
Value
284 kg N/ha per yeare0
Qualifier
P < 0.001e0
Comparator
grass-only swardse0

Verbatim

e0

“The grass-white clover swards yielded significantly higher herbage (10,166 kg DM/ha per year) and N (284 kg N/ha per year) than the grass-only swards (P < 0.001)”

✓ located verbatim in the source · abstract, characters 1148–1309 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

Background N mineralisation of 185 kg N/ha arises from the soil organic matter in grass-only and grass-clover swards receiving zero N.

Taxon
grass-only and grass-clover swardse1
Outcome
N mineralisatione0
Value
185 kg N/hae0
Qualifier
from the organic matter content of the soile0

Verbatim

e0

“The results provide an indication of background N mineralisation of 185 kg N/ha from the organic matter content of the soil”

✓ located verbatim in the source · abstract, characters 1311–1434 · show in text ↑
e1

“within grass-only and grass-clover swards receiving zero N”

✓ located verbatim in the source · abstract, characters 1497–1555 · show in text ↑
Observationauthor interpretationoriginal resultmeasured

Claim · model-written summary

White clover fixed 99 kg N/ha biologically in zero-N swards.

Taxon
white cloverTrifolium repense0
Outcome
biological N fixatione0
Value
99 kg N/hae0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium repens
Trifolium repens · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“biological N fixation of 99 kg N/ha from the white clover”

✓ located verbatim in the source · abstract, characters 1439–1496 · show in text ↑