Abstract
BACKGROUND: The storage of lipids is a crucial mechanism conserved across diverse organisms. Lipid droplets (LDs) are important organelles for storing lipids in cells, and there are many proteins interspersed with their outer phospholipid membranes. SEIPIN, as a LD protein, has been reported to influence the size and number of LDs, and to affect neutral lipid storage in Arabidopsis. RESULTS: In Brassica napus (B. napus), there are three SEIPIN genes, including SEIPIN1 (4 copies), SEIPIN2 (4 copies), and SEIPIN3 (2 copies). The transcriptome databases show that BnaSEIPIN1 only has high expression in late developing seeds, BnaSEIPIN2 has low expression almost in all tissues, while BnaSEIPIN3 has negligible expression across all tissues. BnaC03.SEIPIN1 and BnaA09.SEIPIN2 were cloned to be overexpressed in B. napus seeds specifically. The results showed that BnaC03.SEIPIN1 increased the oil content of seeds by 4.3-5.4% and the thousand-seed weight by 0.43-0.49 g. However, no significant effect of BnaA09.SEIPIN2 on oil content was observed. Further studies revealed that BnaC03.SEIPIN1 significantly increased the number of small and large LDs, but not affected the LDs size. The qRT-PCR results showed that the expression levels of some key genes related to lipid synthesis and storage were altered significantly between overexpression lines and WT. CONCLUSIONS: Taking together, above results reveal that SEIPIN1 improves seed oil content accumulation by the number of small and larger LDs, but not by influencing LDs size. Our results provide a new understanding on SEIPIN regulating seed oil accumulation in Brassica napus.
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Claims 11
Claim · model-written summary
Overexpressing BnaC03.SEIPIN1 increased seed oil content by 4.3-5.4% relative to wild type.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napust0 - Treatment
- overexpressede6
- Gene
- BnaC03.SEIPIN1e0
- Outcome
- oil content of seedse0
- Direction
- increasede0
- Value
- 4.3-5.4%e0
- Comparator
- WTe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
t0“in Brassica napus”
e0“The results showed that BnaC03.SEIPIN1 increased the oil content of seeds by 4.3-5.4% and the thousand-seed weight by 0.43-0.49 g”
e3“the expression levels of some key genes related to lipid synthesis and storage were altered significantly between overexpression lines and WT”
e6“BnaC03.SEIPIN1 and BnaA09.SEIPIN2 were cloned to be overexpressed in B. napus seeds specifically”
Claim · model-written summary
Overexpressing BnaC03.SEIPIN1 increased thousand-seed weight by 0.43-0.49 g relative to wild type.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- overexpressede6
- Gene
- BnaC03.SEIPIN1e0
- Outcome
- thousand-seed weighte0
- Direction
- increasede0
- Value
- 0.43-0.49 ge0
- Comparator
- WTe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Brassica napus”
e0“The results showed that BnaC03.SEIPIN1 increased the oil content of seeds by 4.3-5.4% and the thousand-seed weight by 0.43-0.49 g”
e3“the expression levels of some key genes related to lipid synthesis and storage were altered significantly between overexpression lines and WT”
e6“BnaC03.SEIPIN1 and BnaA09.SEIPIN2 were cloned to be overexpressed in B. napus seeds specifically”
Claim · model-written summary
Overexpressing BnaA09.SEIPIN2 had no significant effect on seed oil content relative to wild type.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- overexpressede6
- Gene
- BnaA09.SEIPIN2e1
- Outcome
- oil contente1
- Direction
- no significant effecte1
- Comparator
- WTe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Brassica napus”
e1“no significant effect of BnaA09.SEIPIN2 on oil content was observed”
e3“the expression levels of some key genes related to lipid synthesis and storage were altered significantly between overexpression lines and WT”
e6“BnaC03.SEIPIN1 and BnaA09.SEIPIN2 were cloned to be overexpressed in B. napus seeds specifically”
Claim · model-written summary
Overexpressing BnaC03.SEIPIN1 significantly increased the number of small and large lipid droplets in seeds.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- overexpressede6
- Gene
- BnaC03.SEIPIN1e2
- Outcome
- number of small and large LDse2
- Direction
- increasede2
- Comparator
- WTe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Brassica napus”
e2“BnaC03.SEIPIN1 significantly increased the number of small and large LDs, but not affected the LDs size”
e3“the expression levels of some key genes related to lipid synthesis and storage were altered significantly between overexpression lines and WT”
e6“BnaC03.SEIPIN1 and BnaA09.SEIPIN2 were cloned to be overexpressed in B. napus seeds specifically”
Claim · model-written summary
Overexpressing BnaC03.SEIPIN1 did not affect lipid droplet size.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- overexpressede6
- Gene
- BnaC03.SEIPIN1e2
- Outcome
- LDs sizee2
- Direction
- no_changeinferrede2
- Comparator
- WTe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Brassica napus”
e2“BnaC03.SEIPIN1 significantly increased the number of small and large LDs, but not affected the LDs size”
e3“the expression levels of some key genes related to lipid synthesis and storage were altered significantly between overexpression lines and WT”
e6“BnaC03.SEIPIN1 and BnaA09.SEIPIN2 were cloned to be overexpressed in B. napus seeds specifically”
Claim · model-written summary
Overexpression lines differed from WT in expression of some lipid synthesis and storage genes.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- overexpression linese3
- Outcome
- expression levels of some key genes related to lipid synthesis and storagee3
- Direction
- alterede3
- Comparator
- WTe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Brassica napus”
e3“the expression levels of some key genes related to lipid synthesis and storage were altered significantly between overexpression lines and WT”
Claim · model-written summary
SEIPIN1 improves seed oil content accumulation through the number of small and larger lipid droplets, not through droplet size.
- Taxon
- Brassica napusBrassica napust0
- Gene
- SEIPIN1e7
- Outcome
- seed oil content accumulatione7
- Qualifier
- by the number of small and larger LDse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Brassica napus”
e7“SEIPIN1 improves seed oil content accumulation by the number of small and larger LDs, but not by influencing LDs size”
Claim · model-written summary
BnaSEIPIN1 has high expression only in late developing seeds.
- Taxon
- Brassica napusBrassica napust0
- Organ / stage
- late developing seedse4
- Gene
- BnaSEIPIN1e4
- Outcome
- high expressione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Brassica napus”
e4“BnaSEIPIN1 only has high expression in late developing seeds, BnaSEIPIN2 has low expression almost in all tissues, while BnaSEIPIN3 has negligible expression across all tissues”
Claim · model-written summary
BnaSEIPIN2 has low expression in almost all tissues.
- Taxon
- Brassica napusBrassica napust0
- Organ / stage
- all tissuese4
- Gene
- BnaSEIPIN2e4
- Outcome
- low expressione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Brassica napus”
e4“BnaSEIPIN1 only has high expression in late developing seeds, BnaSEIPIN2 has low expression almost in all tissues, while BnaSEIPIN3 has negligible expression across all tissues”
Claim · model-written summary
BnaSEIPIN3 has negligible expression across all tissues.
- Taxon
- Brassica napusBrassica napust0
- Organ / stage
- across all tissuese4
- Gene
- BnaSEIPIN3e4
- Outcome
- negligible expressione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Brassica napus”
e4“BnaSEIPIN1 only has high expression in late developing seeds, BnaSEIPIN2 has low expression almost in all tissues, while BnaSEIPIN3 has negligible expression across all tissues”
Claim · model-written summary
Brassica napus has three SEIPIN genes, with SEIPIN1 and SEIPIN2 at four copies each and SEIPIN3 at two.
- Taxon
- Brassica napusBrassica napust0
- Outcome
- SEIPIN genese5
- Qualifier
- SEIPIN1 (4 copies), SEIPIN2 (4 copies), and SEIPIN3 (2 copies)e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Brassica napus”
e5“there are three SEIPIN genes, including SEIPIN1 (4 copies), SEIPIN2 (4 copies), and SEIPIN3 (2 copies)”