Abstract
Soybean mosaic virus (SMV) is a major constraint on global soybean (Glycine max (L.) Merr.) production, causing substantial economic losses worldwide. Despite these losses, the potential of resistance genes as a solution remains largely unexplored. In this study, the COPPER CHAPERONE FOR SUPEROXIDE DISMUTASE (GmCCS) was initially employed as a bait to screen the soybean cDNA library, leading to the identification of a protein homologous to Arabidopsis thaliana COP9 signalosome complex subunit 5B (AtCSN5B), designated as GmCSN5B. Quantitative real-time PCR (qRT-PCR) analysis revealed differential expression of GmCSN5B in the SMV-resistant (Qihuang No.1, QH) and susceptible (Nannong 1138-2, NN) variety following SMV-SC3 strain inoculation. Knockdown of GmCSN5B via Bean pod mottle virus (BPMV)-induced gene silencing (VIGS) significantly enhanced SMV resistance compared to control plants. This work further demonstrated that GmCSN5B can interact with the downstream GmVTC1 protein, which was potentially associated with ascorbic acid (AsA; Vitamin C) synthesis. Moreover, GmVTC1 also responded to SMV infection, and its knockdown led to a reduction in endogenous AsA levels within the host, thereby compromising the plant’s resistance to SMV. Together, these findings suggest that the GmCCS-GmCSN5B-GmVTC1 pathway in soybean modulates host resistance to SMV through the regulation of AsA synthesis.
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Claims 8
Claim · model-written summary
A GmCCS bait screen identified the protein GmCSN5B.
- Taxon
- soybean
Glycine maxe0 - Gene
- GmCSN5Be0
- Outcome
- identification of a protein homologous to Arabidopsis thaliana COP9 signalosome complex subunit 5Be0
- Comparator
- AtCSN5Be0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“COPPER CHAPERONE FOR SUPEROXIDE DISMUTASE (GmCCS) was initially employed as a bait to screen the soybean cDNA library, leading to the identification of a protein homologous to Arabidopsis thaliana COP9 signalosome complex subunit 5B (AtCSN5B), designated as GmCSN5B”
Claim · model-written summary
GmCSN5B expression differed between resistant Qihuang No.1 and susceptible Nannong 1138-2 after SMV-SC3 inoculation.
- Taxon
- SoybeanGlycine maxt0
- Material
- Qihuang No.1, QHe0
- Treatment
- SMV-SC3 strain inoculatione0
- Interactor
- SMV-SC3e0pathogen
- Gene
- GmCSN5Be0
- Outcome
- differential expression of GmCSN5Be0
- Comparator
- Nannong 1138-2, NNe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“differential expression of GmCSN5B in the SMV-resistant (Qihuang No.1, QH) and susceptible (Nannong 1138-2, NN) variety following SMV-SC3 strain inoculation”
t0“Characterization of the Soybean”
Claim · model-written summary
Silencing GmCSN5B significantly enhanced soybean SMV resistance relative to controls.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- Knockdown of GmCSN5B via Bean pod mottle virus (BPMV)-induced gene silencing (VIGS)e0
- Interactor
- SMVe0pathogen
- Gene
- GmCSN5Be0
- Outcome
- SMV resistancee0
- Direction
- enhancede0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Characterization of the Soybean”
e0“Knockdown of GmCSN5B via Bean pod mottle virus (BPMV)-induced gene silencing (VIGS) significantly enhanced SMV resistance compared to control plants”
Claim · model-written summary
GmCSN5B interacted with GmVTC1.
- Taxon
- SoybeanGlycine maxt0
- Gene
- GmCSN5Be0
- Outcome
- interact with the downstream GmVTC1 proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Characterization of the Soybean”
e0“GmCSN5B can interact with the downstream GmVTC1 protein”
Claim · model-written summary
GmVTC1 responded to SMV infection.
- Taxon
- SoybeanGlycine maxt0
- Interactor
- SMVe0pathogen
- Gene
- GmVTC1e0
- Outcome
- responded to SMV infectione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Characterization of the Soybean”
e0“GmVTC1 also responded to SMV infection”
Claim · model-written summary
GmVTC1 knockdown reduced endogenous AsA levels in soybean.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- knockdowne0
- Gene
- GmVTC1t1
- Outcome
- endogenous AsA levelse0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Characterization of the Soybean”
t1“GmVTC1”
e0“its knockdown led to a reduction in endogenous AsA levels within the host”
Claim · model-written summary
GmVTC1 knockdown compromised soybean resistance to SMV.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- knockdowne0
- Interactor
- SMVe0pathogen
- Gene
- GmVTC1e0
- Outcome
- plant’s resistance to SMVe0
- Direction
- compromisinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Characterization of the Soybean”
e0“GmVTC1 also responded to SMV infection, and its knockdown led to a reduction in endogenous AsA levels within the host, thereby compromising the plant’s resistance to SMV”
Claim · model-written summary
The authors propose that the GmCCS-GmCSN5B-GmVTC1 pathway regulates AsA synthesis to modulate soybean resistance to SMV.
- Taxon
- soybeanGlycine maxe0
- Interactor
- SMVe0pathogen
- Gene
- GmCCS-GmCSN5B-GmVTC1e0
- Compound
- AsAe0
- Outcome
- host resistance to SMVe0
- Qualifier
- regulation of AsA synthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmCCS-GmCSN5B-GmVTC1 pathway in soybean modulates host resistance to SMV through the regulation of AsA synthesis”
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