Abstract
Abstract Background Verticillium wilt, commonly known as cotton cancer, is a devastating disease that significantly reduces cotton yield and fiber quality worldwide. The nuclear pore complex (NPC), composed of nucleoporins (Nups), plays a crucial role in plant disease resistance by mediating nucleocytoplasmic transport and selective nuclear signaling that are key components of pathogen defense. Given the critical role of nucleoporins in plant immunity, their specific roles in cotton remain poorly understood, highlighting the need for a comprehensive bioinformatics analysis to clarify their contributions to disease resistance in cotton. Results This study identified a total of 42 Nup genes across five cotton species. Phylogenetic analysis revealed divergence of these genes into six distinct subgroups during evolution. Analysis of cis -acting elements indicated that Nup gene expression may be regulated by salicylic acid (SA), jasmonic acid (JA), and temperature, impacting disease resistance. Collinearity and chromosomal mapping demonstrated strong conservation of Nup gene functions across species. Furthermore, virus-induced gene silencing (VIGS) and quantitative real-time polymerase chain reaction (RT-qPCR) showed that silencing of Ghnup160 significantly reduced cotton resistance to Verticillium wilt. Conclusions Our study suggests an important role of Ghnup160 in the cotton defense pathway and provides functional insights into the Ghnup160 gene and lays the groundwork for further investigation into Nup-mediated resistance mechanisms in cotton.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
Forty-two Nup genes were identified across five cotton species.
- Taxon
- cotton
Gossypium hirsutume0 - Gene
- Nupe0
- Outcome
- Nup genese0
- Value
- 42e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This study identified a total of 42 Nup genes across five cotton species”
Claim · model-written summary
The identified Nup genes diverged into six subgroups.
- Taxon
- cottonGossypium hirsutume1
- Gene
- Nupe1
- Outcome
- subgroupse0
- Value
- sixe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phylogenetic analysis revealed divergence of these genes into six distinct subgroups during evolution”
e1“Nup genes across five cotton species”
Claim · model-written summary
Cis-element analysis suggested that salicylic acid, jasmonic acid and temperature may regulate Nup expression.
- Taxon
- cottonGossypium hirsutume1
- Gene
- Nupe0
- Compound
- salicylic acid (SA), jasmonic acid (JA)e0
- Outcome
- Nup gene expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analysis of cis -acting elements indicated that Nup gene expression may be regulated by salicylic acid (SA), jasmonic acid (JA), and temperature”
e1“five cotton species”
Claim · model-written summary
Collinearity and chromosomal mapping showed strong conservation of Nup gene functions across cotton species.
- Taxon
- cottonGossypium hirsutume1
- Gene
- Nupe0
- Outcome
- conservation of Nup gene functionse0
- Direction
- stronge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Collinearity and chromosomal mapping demonstrated strong conservation of Nup gene functions across species”
e1“five cotton species”
Claim · model-written summary
Silencing Ghnup160 significantly reduced cotton resistance to Verticillium wilt.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- silencing of Ghnup160e0
- Interactor
- VerticilliumVerticilliume0pathogen
- Gene
- Ghnup160e0
- Outcome
- cotton resistance to Verticillium wilte0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of Ghnup160 significantly reduced cotton resistance to Verticillium wilt”
The same questions in other papers
loss of function · Verticillium · Gossypium hirsutum (cotton)Positive and negative results
7 papers in the same direction as this paper · 7 in the opposite direction
- positive
- positive
- positive
11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.