Abstract
ABSTRACT Wheat is the most important cereal worldwide. Germination assays are widely employed to assess seed quality and evaluate the response of diverse genotypes to abiotic stresses. Advancements in 3D printing technology enable the production of customized germination supports. The objective of this study was to develop and validate a 3D‐printed plate for seed germination by assessing its effectiveness for the screening of drought tolerance and herbicide resistance in different wheat genotypes. Germination experiments were conducted with different concentrations of polyethylene glycol (PEG) or imazethapyr herbicide. The 3D‐printed plate improves wheat seed germination when compared to the traditional paper‐based method. PEG treatment significantly reduced ( p < 0.05) shoot length in all genotypes, with two mutant lines displaying higher tolerance to 15% PEG than the wild type. In the herbicide assay, shoot length, green area and root length were significantly inhibited under increased concentrations of imazethapyr, exhibiting genotype‐dependent responses. The 3D‐printed plate proved to be a valuable tool for drought tolerance characterization using PEG and enabled the identification of wheat genotypes with varying levels of resistance to the imazethapyr herbicide. The germination plate developed in this study offers potential for evaluating a broad spectrum of abiotic stresses in wheat.
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Claims 6
Claim · model-written summary
The 3D-printed plate improved wheat germination compared with paper.
- Taxon
- wheat
Triticum aestivume0 - Outcome
- wheat seed germinatione0
- Direction
- improvese0
- Comparator
- traditional paper‐based methode0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 3D‐printed plate improves wheat seed germination when compared to the traditional paper‐based method”
Claim · model-written summary
PEG significantly reduced wheat shoot length across genotypes.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- PEG treatmente0
- Outcome
- shoot lengthe0
- Direction
- reducede0
- Qualifier
- all genotypese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PEG treatment significantly reduced ( p < 0.05) shoot length in all genotypes”
t0“Wheat”
Claim · model-written summary
Two mutant wheat lines tolerated 15% PEG better than wild type.
- Taxon
- WheatTriticum aestivumt0
- Material
- two mutant linese0
- Treatment
- 15% PEGe0
- Outcome
- tolerancee0
- Direction
- highere0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two mutant lines displaying higher tolerance to 15% PEG than the wild type”
t0“Wheat”
Claim · model-written summary
Increasing imazethapyr concentrations significantly inhibited wheat shoot length, green area and root length with genotype-dependent responses.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- increased concentrations of imazethapyre0
- Outcome
- shoot length, green area and root lengthe0
- Direction
- inhibitede0
- Qualifier
- genotype‐dependent responsese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shoot length, green area and root length were significantly inhibited under increased concentrations of imazethapyr, exhibiting genotype‐dependent responses”
t0“Wheat”
Claim · model-written summary
The plate enabled characterization of wheat drought tolerance using PEG.
- Taxon
- WheatTriticum aestivumt0
- Outcome
- drought tolerance characterizatione0
- Value
- PEGe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 3D‐printed plate proved to be a valuable tool for drought tolerance characterization using PEG”
t0“Wheat”
Claim · model-written summary
The plate identified wheat genotypes with differing resistance to imazethapyr.
- Taxon
- wheatTriticum aestivume0
- Outcome
- identification of wheat genotypes with varying levels of resistancee0
- Value
- imazethapyr herbicidee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enabled the identification of wheat genotypes with varying levels of resistance to the imazethapyr herbicide”
The same questions in other papers
poly(ethylene glycol) · growth · Triticum aestivum (wheat)Positive and negative results
16 papers in the same direction as this paper · 6 in the opposite direction
- negative
- positive
- positive
19 papers more in this group
See all 23 papers →Imazethapyr · growth · Triticum aestivum (wheat)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.