Abstract
Abstract Pea (Pisum sativum L.) is one of the most important edible legumes in China, with both planting area and total yield ranking among the highest in the world. Fusarium wilt, caused by Fusarium oxysporum f. sp. pisi (Fop), is a severe factor limiting pea production. The deployment of resistant pea cultivars is the most effective and sustainable strategy for controlling this disease. In the present study, a novel resistance gene PsFwC9, conferring resistance to Fop race 5, was identified in the resistant pure line Chengwan 9-8 (CW9-8), and its candidate gene Psat4g213640 was characterized and functionally validated to be associated with disease resistance. Genetic analysis of the F₂ population derived from the cross between the resistant parent CW9-8 and the susceptible parent Chengwan 9-1 (CW9-1) revealed that PsFwC9 was controlled by a single dominant gene. Based on whole-genome resequencing, bulked segregant analysis sequencing (BSA-seq), and fine mapping, PsFwC9 was localized to an 817.06 kb region on chromosome 4 (i.e., linkage group IV, chr4LG4), flanked by KASP markers A016508 and A016511, and co-segregated with four markers. Haplotype analysis revealed that only the marker A016615 was significantly associated with Fusarium wilt resistance, and this marker was designated as a diagnostic marker for PsFwC9. Marker A016615 was located at 425,699,725 bp on chr4LG4, corresponding to the 277 bp within Psat4g213640, where a “G/A” single-nucleotide polymorphism caused an amino acid substitution leading to an alteration in protein structure, therefore Psat4g213640 was identified as PsFwC9 candidate gene. Quantitative real-time PCR analysis showed no significant difference in the expression levels of Psat4g213640 between CW9-8 and CW9-1. Overexpression of the candidate gene Psat4g213640CW9-8 in the hairy root system significantly enhanced the resistance of CW9-1 to Fusarium wilt, whereas RNA interference-mediated silencing of Psat4g213640CW9-8 reduced the resistance of CW9-8, indicating that Psat4g213640CW9-8 played a crucial role in pea resistance to Fusarium wilt. In addition, subcellular localization showed that the protein encoded by Psat4g213640 was targeted to the endoplasmic reticulum. Collectively, these findings not only enriched the gene resources for disease resistance in pea and provided an important foundation for elucidating the molecular mechanism of PsFwC9-mediated resistance, but also provided important technical support for the practical application of molecular breeding for disease resistance in pea.
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Claims 10
Claim · model-written summary
A novel resistance gene PsFwC9 conferring resistance to Fop race 5 was identified in the resistant pea pure line Chengwan 9-8 (CW9-8).
- Taxon
- Pisum sativum
Lathyrus oleraceuse0 - Material
- Chengwan 9-8e1
- Interactor
- Fop race 5e1pathogen
- Gene
- PsFwC9e1
- Outcome
- resistance to Fop race 5e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pisum sativum”
e1“a novel resistance gene PsFwC9, conferring resistance to Fop race 5, was identified in the resistant pure line Chengwan 9-8 (CW9-8)”
Claim · model-written summary
Genetic analysis of the F2 population from the resistant CW9-8 by susceptible CW9-1 cross showed PsFwC9 was controlled by a single dominant gene.
- Taxon
- Pisum sativumLathyrus oleraceuse0
- Material
- F₂ populatione1
- Gene
- PsFwC9e1
- Outcome
- controlled by a single dominant genee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pisum sativum”
e1“Genetic analysis of the F₂ population derived from the cross between the resistant parent CW9-8 and the susceptible parent Chengwan 9-1 (CW9-1) revealed that PsFwC9 was controlled by a single dominant gene”
Claim · model-written summary
PsFwC9 was localized by resequencing, BSA-seq and fine mapping to an 817.06 kb region on chromosome 4 flanked by KASP markers A016508 and A016511.
- Taxon
- Pisum sativumLathyrus oleraceuse0
- Gene
- PsFwC9e1
- Outcome
- localized to an 817.06 kb region on chromosome 4e1
- Value
- 817.06 kbe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pisum sativum”
e1“PsFwC9 was localized to an 817.06 kb region on chromosome 4 (i.e., linkage group IV, chr4LG4), flanked by KASP markers A016508 and A016511”
Claim · model-written summary
Haplotype analysis showed that only marker A016615 was significantly associated with Fusarium wilt resistance.
- Taxon
- Pisum sativumLathyrus oleraceuse0
- Gene
- marker A016615e1
- Outcome
- Fusarium wilt resistancee1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pisum sativum”
e1“only the marker A016615 was significantly associated with Fusarium wilt resistance”
Claim · model-written summary
Marker A016615 was designated as a diagnostic marker for PsFwC9.
- Taxon
- Pisum sativumLathyrus oleraceuse0
- Gene
- PsFwC9e1
- Outcome
- designated as a diagnostic marker for PsFwC9e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pisum sativum”
e1“this marker was designated as a diagnostic marker for PsFwC9”
Claim · model-written summary
A G/A single-nucleotide polymorphism at 277 bp within Psat4g213640 caused an amino acid substitution altering protein structure.
- Taxon
- Pisum sativumLathyrus oleraceuse0
- Gene
- Psat4g213640e1
- Outcome
- amino acid substitution leading to an alteration in protein structuree1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pisum sativum”
e1“the 277 bp within Psat4g213640, where a “G/A” single-nucleotide polymorphism caused an amino acid substitution leading to an alteration in protein structure”
Claim · model-written summary
Quantitative real-time PCR showed no significant difference in Psat4g213640 expression between CW9-8 and CW9-1.
- Taxon
- Pisum sativumLathyrus oleraceuse0
- Material
- CW9-8 and CW9-1e1
- Gene
- Psat4g213640e1
- Outcome
- expression levels of Psat4g213640e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pisum sativum”
e1“no significant difference in the expression levels of Psat4g213640 between CW9-8 and CW9-1”
Claim · model-written summary
Overexpression of Psat4g213640CW9-8 in the hairy root system significantly enhanced the resistance of CW9-1 to Fusarium wilt.
- Taxon
- Pisum sativumLathyrus oleraceuse0
- Material
- CW9-1e1
- Treatment
- Overexpression of the candidate gene Psat4g213640CW9-8 in the hairy root systeme1
- Gene
- Psat4g213640CW9-8e1
- Outcome
- resistance of CW9-1 to Fusarium wilte1
- Direction
- enhancede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pisum sativum”
e1“Overexpression of the candidate gene Psat4g213640CW9-8 in the hairy root system significantly enhanced the resistance of CW9-1 to Fusarium wilt”
Claim · model-written summary
RNA interference-mediated silencing of Psat4g213640CW9-8 reduced the resistance of CW9-8 to Fusarium wilt.
- Taxon
- Pisum sativumLathyrus oleraceuse0
- Material
- CW9-8e1
- Treatment
- RNA interference-mediated silencing of Psat4g213640CW9-8e1
- Gene
- Psat4g213640CW9-8e1
- Outcome
- resistance of CW9-8e1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pisum sativum”
e1“RNA interference-mediated silencing of Psat4g213640CW9-8 reduced the resistance of CW9-8”
Claim · model-written summary
Subcellular localization showed that the protein encoded by Psat4g213640 was targeted to the endoplasmic reticulum.
- Taxon
- Pisum sativumLathyrus oleraceuse0
- Gene
- Psat4g213640e1
- Outcome
- targeted to the endoplasmic reticulume1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pisum sativum”
e1“the protein encoded by Psat4g213640 was targeted to the endoplasmic reticulum”
The same questions in other papers
loss of function · disease · Lathyrus oleraceus (pea)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- no effect
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.