Abstract
Banana productivity is severely limited by drought, yet the molecular basis of drought adaptation and endophyte-mediated stress alleviation remains poorly understood. Here, we performed a genome-wide analysis of the SQUAMOSA promoter-binding protein-like (SPL) transcription factor family in Musa acuminata and examined their transcriptional responses to drought stress and Serendipita indica inoculation. We identified 38 MaSPL genes, all encoding proteins with the conserved SBP domain and predicted nuclear localization. Phylogenetic, motif, gene structure, and collinearity analyses indicated that MaSPL genes are evolutionarily conserved, unevenly distributed across chromosomes, and expanded primarily through segmental duplication under purifying selection. Promoter analysis showed several cis-acting elements and transcription factor binding sites related to light, phytohormone, and stress signaling. Ten MaSPL genes were predicted as putative targets of miR156. qRT-PCR analysis showed that drought stress markedly downregulated the tested MaSPL genes, whereas miR156a expression increased, supporting an inverse regulatory relationship. Under drought, S. indica inoculation enhanced expression of most tested MaSPLs, restoring transcript accumulation while reducing miR156a to near-basal levels. Notable responses were observed in members of the MaSPL2, MaSPL9, and MaSPL13, respectively. S. indica improves drought tolerance by enhancing antioxidant defenses, reducing oxidative stress, and preserving photosynthetic and osmotic stability. Taken together, our results demonstrate that S. indica confers drought resilience in banana by counteracting drought-induced repression of MaSPL genes via the miR156–SPL module and by strengthening key physiological defense mechanisms.
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Claims 12
Claim · model-written summary
Thirty-eight MaSPL genes encode conserved SBP-domain proteins with predicted nuclear localization.
- Taxon
- Musa acuminata
Musa acuminatat0 - Gene
- MaSPL genese0
- Outcome
- 38 MaSPL genese0
- Value
- 38e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We identified 38 MaSPL genes, all encoding proteins with the conserved SBP domain and predicted nuclear localization.”
t0“Musa acuminata”
Claim · model-written summary
MaSPL genes are evolutionarily conserved, unevenly distributed across chromosomes, and expanded mainly through segmental duplication under purifying selection.
- Taxon
- Musa acuminataMusa acuminatat0
- Gene
- MaSPL genese0
- Outcome
- evolutionarily conserved, unevenly distributed across chromosomes, and expanded primarily through segmental duplication under purifying selectione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MaSPL genes are evolutionarily conserved, unevenly distributed across chromosomes, and expanded primarily through segmental duplication under purifying selection.”
t0“Musa acuminata”
Claim · model-written summary
MaSPL promoters have cis-acting elements and transcription factor binding sites related to light, phytohormone, and stress signaling.
- Taxon
- Musa acuminataMusa acuminatat0
- Outcome
- cis-acting elements and transcription factor binding sitese0
- Qualifier
- light, phytohormone, and stress signalinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Promoter analysis showed several cis-acting elements and transcription factor binding sites related to light, phytohormone, and stress signaling.”
t0“Musa acuminata”
Claim · model-written summary
Ten MaSPL genes are predicted targets of miR156.
- Taxon
- Musa acuminataMusa acuminatat0
- Gene
- MaSPL genese0
- Outcome
- putative targets of miR156e0
- Value
- Tene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ten MaSPL genes were predicted as putative targets of miR156.”
t0“Musa acuminata”
Claim · model-written summary
Drought markedly downregulated tested MaSPL genes.
- Taxon
- Musa acuminataMusa acuminatat0
- Treatment
- drought stresse0
- Outcome
- tested MaSPL genese0
- Direction
- downregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“drought stress markedly downregulated the tested MaSPL genes”
t0“Musa acuminata”
Claim · model-written summary
Drought increased miR156a expression.
- Taxon
- Musa acuminataMusa acuminatat0
- Treatment
- drought stresse0
- Outcome
- miR156a expressione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“qRT-PCR analysis showed that drought stress markedly downregulated the tested MaSPL genes, whereas miR156a expression increased”
t0“Musa acuminata”
Claim · model-written summary
Under drought, S. indica inoculation enhanced expression of most tested MaSPLs.
- Taxon
- Musa acuminataMusa acuminatat0
- Treatment
- S. indica inoculatione0
- Interactor
- S. indicaSerendipita indicae0symbiont
- Outcome
- expression of most tested MaSPLse0
- Direction
- enhancede0
- Qualifier
- Under droughte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under drought, S. indica inoculation enhanced expression of most tested MaSPLs, restoring transcript accumulation while reducing miR156a to near-basal levels.”
t0“Musa acuminata”
Claim · model-written summary
Under drought, S. indica inoculation reduced miR156a to near-basal levels.
- Taxon
- Musa acuminataMusa acuminatat0
- Treatment
- S. indica inoculatione0
- Interactor
- S. indicaSerendipita indicae0symbiont
- Outcome
- miR156ae0
- Direction
- reducinge0
- Qualifier
- Under droughte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under drought, S. indica inoculation enhanced expression of most tested MaSPLs, restoring transcript accumulation while reducing miR156a to near-basal levels.”
t0“Musa acuminata”
Claim · model-written summary
S. indica improves drought tolerance by enhancing antioxidant defenses.
- Taxon
- Musa acuminataMusa acuminatat0
- Interactor
- S. indicaSerendipita indicae0symbiont
- Outcome
- drought tolerancee0
- Qualifier
- enhancing antioxidant defensese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“S. indica improves drought tolerance by enhancing antioxidant defenses, reducing oxidative stress, and preserving photosynthetic and osmotic stability.”
t0“Musa acuminata”
Claim · model-written summary
S. indica reduces oxidative stress in drought-stressed banana.
- Taxon
- Musa acuminataMusa acuminatat0
- Treatment
- S. indicae0
- Interactor
- S. indicaSerendipita indicae0symbiont
- Outcome
- oxidative stresse0
- Direction
- reducinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“S. indica improves drought tolerance by enhancing antioxidant defenses, reducing oxidative stress, and preserving photosynthetic and osmotic stability.”
t0“Musa acuminata”
Claim · model-written summary
S. indica preserves photosynthetic and osmotic stability under drought.
- Taxon
- Musa acuminataMusa acuminatat0
- Interactor
- S. indicaSerendipita indicae0symbiont
- Outcome
- photosynthetic and osmotic stabilitye0
- Qualifier
- preservinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“S. indica improves drought tolerance by enhancing antioxidant defenses, reducing oxidative stress, and preserving photosynthetic and osmotic stability.”
t0“Musa acuminata”
Claim · model-written summary
The authors attribute S. indica-induced drought resilience to counteraction of MaSPL repression through the miR156–SPL module.
Verbatim
e0“S. indica confers drought resilience in banana by counteracting drought-induced repression of MaSPL genes via the miR156–SPL module”
The same questions in other papers
inoculation · molecular: expression · Musa acuminata (banana)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.