Paper · 2026 · Frontiers in Plant Science

Effects of planting density and row spacing configuration on dry matter accumulation and distribution, nutrient content, and yield in cotton

Ziwei Bai; Nan Cao; Ming Wen; et al.

12
claims
25
verbatim quotes
0
citations
2
research areas
✓
Open access · cc-by

Abstract

Background Dry matter accumulation and distribution constitute the essential material basis for crop yield formation, directly impacting both yield and quality development. Nonetheless, the effects of planting density on dry matter accumulation, distribution, and nutrient uptake in cotton across various row spacing configurations remain ambiguous. Methods This study conducted a two-year field experiment utilizing the cotton cultivar “Tahe 2”. In 2024, a single-factor trial was adopted with three row spacing treatments: same row spacing (S3), wide-ultra-narrow row spacing (S4), and wide-narrow row planting (S6). In 2025, a two-factor split-plot design was used: main plots were the three row spacing patterns (S3, S4, S6), and subplots were three planting densities: 135 000 plants ha -1 (D1), 180 000 plants ha -1 (D2), 225 000 plants ha - ¹ (D3). We investigated leaf area index (LAI), dry matter accumulation and distribution, nutrient content, and yield. Results In 2024, S6 showed the highest leaf area index (LAI) and dry matter accumulation ( p < 0.05). In 2025, LAI and dry matter accumulation increased significantly with increasing plant density ( p < 0.05). The dry matter partitioning ratio to reproductive organs increased with growth. Row spacing and planting density significantly affected leaf nutrient contents; upper-leaf nitrogen (N) and potassium (K) first increased then decreased, while middle- and lower-leaf N and leaf phosphorus (P) at all positions declined gradually. Boll number per plant decreased with increasing density, whereas yield was significantly affected by their interaction ( p < 0.05). The highest yield was found in S6 in 2024 and S6D2 in 2025. Two-year experiments indicated that S6 combined with 180,000 plants ha - ¹ optimized population structure, improved resource use efficiency, and increased cotton yield. Conclusion Among the three row spacing configurations tested, the medium planting density of 180,000 plants ha -1 under the wide-narrow row planting pattern (S6) optimized the leaf area index, enhanced population dry matter accumulation, and improved distribution to reproductive organs, thereby maximizing seed cotton yield, which reached up to 6420.47 kg·ha -1 .

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 12

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

LAI and dry matter accumulation increased significantly with increasing plant density in 2025.

Treatment
increasing plant densitye1
Outcome
LAI and dry matter accumulatione1
Direction
increasede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gossypium hirsutum
Gossypium hirsutum · TaxonChriKo · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“cotton”

✓ located verbatim in the source · title, characters 134–140 · show in text ↑
e1

“LAI and dry matter accumulation increased significantly with increasing plant density”

✓ located verbatim in the source · abstract, characters 1077–1162 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

S6 showed the highest leaf area index and dry matter accumulation in 2024.

Treatment
S6e2
Outcome
leaf area index (LAI) and dry matter accumulatione2
Direction
higheste2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gossypium hirsutum
Gossypium hirsutum · TaxonChriKo · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“cotton”

✓ located verbatim in the source · title, characters 134–140 · show in text ↑
e2

“S6 showed the highest leaf area index (LAI) and dry matter accumulation”

✓ located verbatim in the source · abstract, characters 983–1054 · show in text ↑
Associationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Row spacing and planting density significantly affected leaf nutrient contents.

Treatment
Row spacing and planting densitye3
Outcome
leaf nutrient contentse3

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gossypium hirsutum
Gossypium hirsutum · TaxonChriKo · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“cotton”

✓ located verbatim in the source · title, characters 134–140 · show in text ↑
e3

“Row spacing and planting density significantly affected leaf nutrient contents”

✓ located verbatim in the source · abstract, characters 1256–1334 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Upper-leaf N and K first increased and then decreased.

Organ / stage
upper-leafe4
Outcome
upper-leaf nitrogen (N) and potassium (K)e4
Direction
first increased then decreasede4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gossypium hirsutum
Gossypium hirsutum · TaxonChriKo · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“cotton”

✓ located verbatim in the source · title, characters 134–140 · show in text ↑
e4

“upper-leaf nitrogen (N) and potassium (K) first increased then decreased”

✓ located verbatim in the source · abstract, characters 1336–1408 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Middle- and lower-leaf N and leaf P at all positions declined gradually.

Organ / stage
middle- and lower-leafe5
Outcome
N and leaf phosphorus (P) at all positionse5
Direction
declined graduallye5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gossypium hirsutum
Gossypium hirsutum · TaxonChriKo · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“cotton”

✓ located verbatim in the source · title, characters 134–140 · show in text ↑
e5

“middle- and lower-leaf N and leaf phosphorus (P) at all positions declined gradually”

✓ located verbatim in the source · abstract, characters 1416–1500 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Boll number per plant decreased with increasing planting density.

Treatment
increasing densitye6
Outcome
Boll number per plante6
Direction
decreasede6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gossypium hirsutum
Gossypium hirsutum · TaxonChriKo · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“cotton”

✓ located verbatim in the source · title, characters 134–140 · show in text ↑
e6

“Boll number per plant decreased with increasing density”

✓ located verbatim in the source · abstract, characters 1502–1557 · show in text ↑
Associationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Yield was significantly affected by the interaction of row spacing and planting density.

Treatment
their interactione7
Outcome
yielde7

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gossypium hirsutum
Gossypium hirsutum · TaxonChriKo · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“cotton”

✓ located verbatim in the source · title, characters 134–140 · show in text ↑
e7

“whereas yield was significantly affected by their interaction”

✓ located verbatim in the source · abstract, characters 1559–1620 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The highest yield was found in S6 in 2024 and S6D2 in 2025.

Treatment
S6e1
Outcome
yielde1
Direction
higheste1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gossypium hirsutum
Gossypium hirsutum · TaxonChriKo · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“cotton”

✓ located verbatim in the source · title, characters 134–140 · show in text ↑
e1

“The highest yield was found in S6 in 2024 and S6D2 in 2025”

✓ located verbatim in the source · abstract, characters 1634–1692 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

S6 combined with 180,000 plants ha-1 increased cotton yield.

Treatment
S6 combined with 180,000 plants ha - ¹e1
Outcome
cotton yielde1
Direction
increasede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gossypium hirsutum
Gossypium hirsutum · TaxonChriKo · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“cotton”

✓ located verbatim in the source · title, characters 134–140 · show in text ↑
e1

“S6 combined with 180,000 plants ha - ¹ optimized population structure, improved resource use efficiency, and increased cotton yield”

✓ located verbatim in the source · abstract, characters 1730–1861 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

S6 combined with 180,000 plants ha-1 improved resource use efficiency.

Treatment
S6 combined with 180,000 plants ha - ¹e1
Outcome
resource use efficiencye1
Direction
improvede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gossypium hirsutum
Gossypium hirsutum · TaxonChriKo · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“cotton”

✓ located verbatim in the source · title, characters 134–140 · show in text ↑
e1

“S6 combined with 180,000 plants ha - ¹ optimized population structure, improved resource use efficiency, and increased cotton yield”

✓ located verbatim in the source · abstract, characters 1730–1861 · show in text ↑
Intervention effectauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

The medium density of 180,000 plants ha-1 under S6 maximized seed cotton yield, reaching up to 6420.47 kg·ha-1.

Treatment
medium planting density of 180,000 plants ha -1 under the wide-narrow row planting pattern (S6)e2
Outcome
seed cotton yielde1
Direction
maximizinge1
Value
6420.47 kg·ha -1e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gossypium hirsutum
Gossypium hirsutum · TaxonChriKo · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“cotton”

✓ located verbatim in the source · title, characters 134–140 · show in text ↑
e1

“thereby maximizing seed cotton yield, which reached up to 6420.47 kg·ha -1”

✓ located verbatim in the source · abstract, characters 2151–2225 · show in text ↑
e2

“the medium planting density of 180,000 plants ha -1 under the wide-narrow row planting pattern (S6)”

✓ located verbatim in the source · abstract, characters 1925–2024 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The dry matter partitioning ratio to reproductive organs increased with growth.

Organ / stage
reproductive organse1
Outcome
dry matter partitioning ratio to reproductive organse1
Direction
increased with growthe1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gossypium hirsutum
Gossypium hirsutum · TaxonChriKo · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“cotton”

✓ located verbatim in the source · title, characters 134–140 · show in text ↑
e1

“The dry matter partitioning ratio to reproductive organs increased with growth”

✓ located verbatim in the source · abstract, characters 1176–1254 · show in text ↑

density · growth · Gossypium hirsutum (cotton)Positive and negative results

46 papers: 39 positive, 7 negative or no effect

38 papers in the same direction as this paper · 7 in the opposite direction

  1. positive
  2. no effect
  3. no effect

42 papers more in this group

See all 46 papers →

density · yield · Gossypium hirsutum (cotton)Positive and negative results

171 papers: 130 positive, 41 negative or no effect

129 papers in the same direction as this paper · 38 in the opposite direction · 3 reporting no difference from an alternative

  1. negative
  2. positive
  3. positive

167 papers more in this group

See all 171 papers →

in vitro · yield · Gossypium hirsutum (cotton)Results agree

15 papers: 15 positive, 0 negative or no effect

14 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

11 papers more in this group

See all 15 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.