Abstract
The role of endogenous salicylic acid (SA), a major signaling molecule, was addressed in relation to the waterlogging (WL) stress response, including redox homeostasis and senescence. Wild-type and salicylate hydroxylase-expressing (NahG) tobacco plants were studied to reveal the stress-related effects of the transgene, which is known to deplete the endogenous SA pool. In control conditions, SA levels of the top leaves of NahG plants were moderately lower than those of wild-type, while SA was considerably reduced in the bottom leaves. WL conditions triggered a rise in H2O2 concentrations in young leaves, which was exaggerated in NahG plants, pointing to a mitigating effect of SA against the stress-associated oxidative burden. The NahG transgenic leaves displayed lower protein levels than their wild-type counterparts, indicating a role of SA in protein retention. In non-stressed NahG plants, young (top) leaves showed an increased level of protein nitration. WL treatment triggered decreased protein contents in the leaves of both genotypes. This coincided with the high H2O2 content of old leaves exceeding that of young leaves in most cases. The expression of the senescence marker gene Cysteine protease 1 was upregulated in WL-stressed bottom leaves. According to this marker, senescence progressed faster in NahG leaves. Links between SA, protein nitration, and leaf senescence were discussed. Additionally, a stimulating effect of the NahG transgene was confirmed on adventitious roots (AR) formation, which may have helped root functions and thus probably contributed to maintaining the growth of the WL-stressed plants. Our results have implications for how endogenous SA levels influence plants in a WL stress situation. According to our findings, the depletion of SA may trigger protein loss and tyrosine nitration, but at the same time accelerates AR formation in WL-stressed tobacco.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 15
Claim · model-written summary
In control conditions, SA levels in top leaves of NahG plants were moderately lower than in wild-type.
- Taxon
- tobacco plants
Nicotiana tabacume0 - Material
- NahGe1
- Organ / stage
- top leavese1
- Outcome
- SA levelse1
- Direction
- lowere1
- Comparator
- wild-typee1
- Setting
- control conditionse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wild-type and salicylate hydroxylase-expressing (NahG) tobacco plants were studied”
e1“In control conditions, SA levels of the top leaves of NahG plants were moderately lower than those of wild-type”
Claim · model-written summary
In control conditions, SA was considerably reduced in the bottom leaves of tobacco.
- Taxon
- tobacco plantsNicotiana tabacume0
- Organ / stage
- bottom leavese2
- Outcome
- SAe2
- Direction
- reducede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wild-type and salicylate hydroxylase-expressing (NahG) tobacco plants were studied”
e2“while SA was considerably reduced in the bottom leaves”
Claim · model-written summary
Waterlogging triggered a rise in H2O2 concentrations in young leaves.
- Taxon
- tobacco plantsNicotiana tabacume0
- Organ / stage
- young leavese3
- Treatment
- WL conditionse3
- Outcome
- H2O2 concentrationse3
- Direction
- risee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wild-type and salicylate hydroxylase-expressing (NahG) tobacco plants were studied”
e3“WL conditions triggered a rise in H2O2 concentrations in young leaves”
Claim · model-written summary
The rise in H2O2 concentrations was exaggerated in NahG plants.
- Taxon
- tobacco plantsNicotiana tabacume0
- Material
- NahGe4
- Outcome
- H2O2 concentrationse3
- Direction
- exaggeratede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wild-type and salicylate hydroxylase-expressing (NahG) tobacco plants were studied”
e3“WL conditions triggered a rise in H2O2 concentrations in young leaves”
e4“which was exaggerated in NahG plants”
Claim · model-written summary
SA appears to mitigate the oxidative burden of waterlogging stress.
- Taxon
- tobacco plantsNicotiana tabacume0
- Compound
- SAe5
- Outcome
- oxidative burdene5
- Qualifier
- mitigating effecte5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wild-type and salicylate hydroxylase-expressing (NahG) tobacco plants were studied”
e5“pointing to a mitigating effect of SA against the stress-associated oxidative burden”
Claim · model-written summary
NahG transgenic leaves displayed lower protein levels than wild-type counterparts.
- Taxon
- tobacco plantsNicotiana tabacume0
- Material
- NahG transgenice6
- Organ / stage
- leavese6
- Outcome
- protein levelse6
- Direction
- lowere6
- Comparator
- wild-type counterpartse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wild-type and salicylate hydroxylase-expressing (NahG) tobacco plants were studied”
e6“The NahG transgenic leaves displayed lower protein levels than their wild-type counterparts”
Claim · model-written summary
SA plays a role in protein retention in tobacco leaves.
- Taxon
- tobacco plantsNicotiana tabacume0
- Compound
- SAe7
- Outcome
- protein retentione7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wild-type and salicylate hydroxylase-expressing (NahG) tobacco plants were studied”
e7“indicating a role of SA in protein retention”
Claim · model-written summary
In non-stressed NahG plants, young top leaves showed an increased level of protein nitration.
- Taxon
- tobacco plantsNicotiana tabacume0
- Material
- NahGe1
- Organ / stage
- young (top) leavese1
- Outcome
- protein nitratione1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wild-type and salicylate hydroxylase-expressing (NahG) tobacco plants were studied”
e1“In non-stressed NahG plants, young (top) leaves showed an increased level of protein nitration”
Claim · model-written summary
Waterlogging treatment decreased protein contents in the leaves of both genotypes.
- Taxon
- tobacco plantsNicotiana tabacume0
- Organ / stage
- leavese1
- Treatment
- WL treatmente1
- Outcome
- protein contentse1
- Direction
- decreasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wild-type and salicylate hydroxylase-expressing (NahG) tobacco plants were studied”
e1“WL treatment triggered decreased protein contents in the leaves of both genotypes”
Claim · model-written summary
The high H2O2 content of old leaves exceeded that of young leaves in most cases under waterlogging.
- Taxon
- tobacco plantsNicotiana tabacume0
- Organ / stage
- old leavese1
- Outcome
- H2O2 content of old leavese1
- Direction
- exceedinge1
- Comparator
- young leavese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wild-type and salicylate hydroxylase-expressing (NahG) tobacco plants were studied”
e1“This coincided with the high H2O2 content of old leaves exceeding that of young leaves in most cases”
Claim · model-written summary
Expression of the senescence marker Cysteine protease 1 was upregulated in waterlogging-stressed bottom leaves.
- Taxon
- tobacco plantsNicotiana tabacume0
- Organ / stage
- bottom leavese1
- Treatment
- WL-stressede1
- Gene
- Cysteine protease 1e1
- Outcome
- expressione1
- Direction
- upregulatede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wild-type and salicylate hydroxylase-expressing (NahG) tobacco plants were studied”
e1“The expression of the senescence marker gene Cysteine protease 1 was upregulated in WL-stressed bottom leaves”
Claim · model-written summary
Senescence progressed faster in NahG leaves, according to the senescence marker.
- Taxon
- tobacco plantsNicotiana tabacume0
- Material
- NahG leavese1
- Outcome
- senescencee1
- Direction
- fastere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wild-type and salicylate hydroxylase-expressing (NahG) tobacco plants were studied”
e1“According to this marker, senescence progressed faster in NahG leaves”
Claim · model-written summary
The NahG transgene had a stimulating effect on adventitious root formation.
- Taxon
- tobacco plantsNicotiana tabacume0
- Treatment
- NahG transgenee1
- Outcome
- adventitious roots (AR) formatione1
- Direction
- stimulatinge1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wild-type and salicylate hydroxylase-expressing (NahG) tobacco plants were studied”
e1“a stimulating effect of the NahG transgene was confirmed on adventitious roots (AR) formation”
Claim · model-written summary
Increased adventitious root formation may have helped root functions and maintained growth of waterlogging-stressed plants.
- Taxon
- tobacco plantsNicotiana tabacume0
- Outcome
- growth of the WL-stressed plantse1
- Qualifier
- which may have helped root functionse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wild-type and salicylate hydroxylase-expressing (NahG) tobacco plants were studied”
e1“which may have helped root functions and thus probably contributed to maintaining the growth of the WL-stressed plants”
Claim · model-written summary
Depletion of SA accelerates adventitious root formation in waterlogging-stressed tobacco.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- depletion of SAe0
- Outcome
- AR formatione1
- Direction
- acceleratese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the depletion of SA may trigger protein loss and tyrosine nitration”
e1“but at the same time accelerates AR formation in WL-stressed tobacco”
The same questions in other papers
flooding · physiology: oxidative · Nicotiana tabacum (tobacco)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- negative
transgenic · growth · Nicotiana tabacum (tobacco)Positive and negative results
22 papers in the same direction as this paper · 15 in the opposite direction
- negative
- negative
- negative
34 papers more in this group
See all 38 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.