Abstract
In this study, an effective regeneration and micropropagation protocol was developed from segregating the F4 industrial hemp genotype Narlısaray. The apical shoot meristem and nodal (from different parts of the plant) explants were cultured at various concentrations of TDZ, BAP or Kin. Shoot regeneration was observed in all treatments, including the control treatment. The maximum number of shoots per explant (4.7) was obtained at 1.0 mg l⁻¹ TDZ, and was statistically different from 0.5 mg l⁻¹ (4.3) and 2.0 mg l⁻¹ TDZ (4.0). The longest shoots (6.5 cm) were recorded on 1.0 mg l-1 TDZ. The position of the nodal explant source on the plant also affected its regeneration ability. The 1st stem node explant was more responsive to in vitro regeneration than the 2nd stem node. All tested concentrations of IBA induced root regeneration, with frequencies ranging from 62.5% to 87.5%. The regenerated plants were successfully acclimatized, reaching a maximum survival rate of 66.7%. This propagation technique can contribute to future hemp rapid breeding programs.
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Claims 6
Claim · model-written summary
Hemp shoot regeneration occurred in all treatments, including control.
- Taxon
- Hemp
Cannabis sativa subsp. sativat0 - Outcome
- Shoot regeneratione0
- Qualifier
- all treatments, including the control treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Hemp (Cannabis sativa)”
e0“Shoot regeneration was observed in all treatments, including the control treatment”
Claim · model-written summary
TDZ at 1.0 mg l⁻¹ gave the maximum 4.7 shoots per explant, significantly different from the 0.5 and 2.0 mg l⁻¹ doses.
- Taxon
- HempCannabis sativa subsp. sativat0
- Treatment
- 1.0 mg l⁻¹ TDZe0
- Outcome
- number of shoots per explante0
- Direction
- maximume0
- Value
- 4.7e0
- Qualifier
- 2.0 mg l⁻¹ TDZ (4.0)e0
- Comparator
- 0.5 mg l⁻¹e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Hemp (Cannabis sativa)”
e0“The maximum number of shoots per explant (4.7) was obtained at 1.0 mg l⁻¹ TDZ, and was statistically different from 0.5 mg l⁻¹ (4.3) and 2.0 mg l⁻¹ TDZ (4.0)”
Claim · model-written summary
TDZ at 1.0 mg l-1 produced the longest shoots, 6.5 cm.
- Taxon
- HempCannabis sativa subsp. sativat0
- Treatment
- 1.0 mg l-1 TDZe0
- Outcome
- shootse0
- Direction
- longeste0
- Value
- 6.5 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Hemp (Cannabis sativa)”
e0“The longest shoots (6.5 cm) were recorded on 1.0 mg l-1 TDZ”
Claim · model-written summary
First stem node explants regenerated more responsively than second stem node explants.
- Taxon
- HempCannabis sativa subsp. sativat0
- Material
- 1st stem node explante0
- Outcome
- in vitro regeneratione0
- Direction
- more responsivee0
- Comparator
- 2nd stem nodee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Hemp (Cannabis sativa)”
e0“The 1st stem node explant was more responsive to in vitro regeneration than the 2nd stem node”
Claim · model-written summary
All tested IBA concentrations induced root regeneration at frequencies from 62.5% to 87.5%.
- Taxon
- HempCannabis sativa subsp. sativat0
- Treatment
- All tested concentrations of IBAe0
- Outcome
- root regeneratione0
- Direction
- inducede0
- Value
- 62.5% to 87.5%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Hemp (Cannabis sativa)”
e0“All tested concentrations of IBA induced root regeneration, with frequencies ranging from 62.5% to 87.5%”
Claim · model-written summary
Regenerated hemp plants acclimatized successfully, reaching maximum survival of 66.7%.
- Taxon
- HempCannabis sativa subsp. sativat0
- Outcome
- survival ratee0
- Direction
- maximume0
- Value
- 66.7%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Hemp (Cannabis sativa)”
e0“The regenerated plants were successfully acclimatized, reaching a maximum survival rate of 66.7%”
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