Abstract
Saccharum spp., a globally important crop for sugar and bioenergy production, suffers substantial yield losses due to drought. Whole-plant studies are complicated by inherent tissue heterogeneity and organ-level interactions. To dissect this complexity, we established a homogeneous suspension cell line from the drought-tolerant cultivar ‘Roc22’. A gradient of polyethylene glycol 6000 (PEG-6000)-induced drought stress (0%, 5%, 10%, 20%) was applied, enabling integrated cytological, physiological, and transcriptomic analysis. Cytological analysis revealed a concentration-dependent decline in cell viability under drought stress, with survival rates dropping to 45.5% after 8 h of 20% PEG treatment. Cells exhibited browning, shrinkage, and ultrastructural damage (affecting the plasma membrane, mitochondria, and nucleus), with severe stress triggering programmed cell death (PCD). Physiological analysis showed that malondialdehyde (MDA) content peaked under 10% PEG treatment (17.16 μmol g −1 FW, representing a 68.55% increase over the control), soluble sugar content accumulated progressively with stress intensity (increasing by 45.64% at 20% PEG), while soluble protein content decreased significantly (a reduction of 82.9% at 20% PEG). Antioxidant enzyme activities responded differentially. Transcriptome profiling identified 9,923 differentially expressed genes (DEGs). Protein-protein interaction (PPI) network analysis pinpointed 21 core candidates, including aquaporins ( e.g. , TIP3-1 , NIP1-1 ), cell wall modifiers ( e.g., XTH23 , CESA1 ), peroxidases ( e.g. , PER25 ), and antioxidant enzymes ( e.g. , APX1 , which was strikingly up-regulated 6.22-fold).This study provides a systematic dissection of th e multi-dimensional drought response network in sugarcane at the cellular level, integrating water regulation, oxidative defense, cell wall remodeling, and cell cycleregulation. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) validation of nine candidate genes confirmed the reliability of the transcriptomic data. Our findings offer deep mechanistic insights and establish a novel “cell model + multi-omics” paradigm for crop stress biology research.
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Claims 11
Claim · model-written summary
Cell viability declined with PEG stress, reaching 45.5% survival after 8 h at 20% PEG.
- Taxon
- sugarcanet0
- Treatment
- 20% PEG treatmente0
- Outcome
- cell viabilitye0
- Direction
- declinee0
- Value
- 45.5%e0
- Qualifier
- after 8 he0
Not reportedComparator
Verbatim
e0“Cytological analysis revealed a concentration-dependent decline in cell viability under drought stress, with survival rates dropping to 45.5% after 8 h of 20% PEG treatment”
t0“sugarcane”
Claim · model-written summary
Stressed cells showed browning, shrinkage and ultrastructural damage.
Verbatim
e0“Cells exhibited browning, shrinkage, and ultrastructural damage (affecting the plasma membrane, mitochondria, and nucleus)”
t0“sugarcane”
Claim · model-written summary
Severe stress triggered programmed cell death.
Not reportedComparatorDirection
Verbatim
e0“severe stress triggering programmed cell death (PCD)”
t0“sugarcane”
Claim · model-written summary
Malondialdehyde content peaked at 17.16 μmol g −1 FW under 10% PEG, 68.55% above control.
Verbatim
e0“malondialdehyde (MDA) content peaked under 10% PEG treatment (17.16 μmol g −1 FW, representing a 68.55% increase over the control)”
t0“sugarcane”
Claim · model-written summary
Soluble sugar content rose progressively with stress intensity, by 45.64% at 20% PEG.
Not reportedComparator
Verbatim
e0“soluble sugar content accumulated progressively with stress intensity (increasing by 45.64% at 20% PEG)”
t0“sugarcane”
Claim · model-written summary
Soluble protein content fell significantly by 82.9% at 20% PEG.
Not reportedComparator
Verbatim
e0“soluble protein content decreased significantly (a reduction of 82.9% at 20% PEG)”
t0“sugarcane”
Claim · model-written summary
Antioxidant enzyme activities responded differentially to stress.
Verbatim
e0“Antioxidant enzyme activities responded differentially”
t0“sugarcane”
Claim · model-written summary
Transcriptome profiling found 9,923 differentially expressed genes.
Verbatim
e0“Transcriptome profiling identified 9,923 differentially expressed genes (DEGs)”
t0“sugarcane”
Claim · model-written summary
PPI analysis identified 21 core candidate genes or proteins.
Verbatim
e0“Protein-protein interaction (PPI) network analysis pinpointed 21 core candidates, including aquaporins ( e.g. , TIP3-1 , NIP1-1 ), cell wall modifiers ( e.g., XTH23 , CESA1 ), peroxidases ( e.g. , PER25 ), and antioxidant enzymes ( e.g. , APX1 , which was strikingly up-regulated 6.22-fold)”
t0“sugarcane”
Claim · model-written summary
APX1 was up-regulated 6.22-fold.
Verbatim
e0“APX1 , which was strikingly up-regulated 6.22-fold”
t0“sugarcane”
Claim · model-written summary
qRT-PCR validation of nine candidate genes supported the transcriptomic data.
Verbatim
e0“Quantitative reverse transcription polymerase chain reaction (qRT-PCR) validation of nine candidate genes confirmed the reliability of the transcriptomic data”
t0“sugarcane”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.