Abstract
Mutants of the monopartite geminivirus beet curly top virus (BCTV) have been screened for infectivity, systemic movement, replication and symptom development in Arabidopsis thaliana. As known by coding for coat protein, R1 mutant was not infectious and did not move systemically. R2, R3 and L2/L3 mutants produced milder symptoms compared to wild type BCTV but the infectivity was reduced by 40% to 60%. R2 ORF is thought to be involved in the regulation of ssDNA and dsDNA accumulation because only dsDNA was accumulated on R2-infected organs. Disruption of ORF L4 resulted in reduced infections, but the viral DNA was accumulated in infected organs from roots to shoot tips as much as wild type BCTV on Sei-O. In addition, 4 mutants did not produce callus-like tissues on infected organs, suggesting that L4 ORF may play a role in the induction of host cell divisions by virus infection. This result was supported by the patterns of mRNA expression and promoter analysis of the cell cycle marker gene, cycl, on Arabidopsis. cycl mRNA was accumulated on symptomatic organs by wild type BCTV infections but not by L4 mutant. We conclude that the BCTV L4 ORF is essential for symptom developments, specially callus-like formation on infected organs.
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Claims 14
Claim · model-written summary
R1 mutant BCTV was not infectious in Arabidopsis.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Material
- R1 mutante1
- Outcome
- not infectiouse1
Not reportedInteractor
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana”
e1“R1 mutant was not infectious and did not move systemically.”
Claim · model-written summary
R1 mutant BCTV failed systemic movement.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- R1 mutante1
- Outcome
- did not move systemicallye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana”
e1“R1 mutant was not infectious and did not move systemically.”
Claim · model-written summary
R2 mutant caused milder symptoms than wild type BCTV.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- R2e2
- Interactor
- BCTV
Beet curly top viruse2pathogen - Outcome
- symptomse2
- Direction
- mildere2
- Comparator
- wild type BCTVe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana”
e2“R2, R3 and L2/L3 mutants produced milder symptoms compared to wild type BCTV but the infectivity was reduced by 40% to 60%.”
Claim · model-written summary
R3 mutant caused milder symptoms than wild type BCTV.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- R3e2
- Interactor
- BCTVBeet curly top viruse2pathogen
- Outcome
- symptomse2
- Direction
- mildere2
- Comparator
- wild type BCTVe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana”
e2“R2, R3 and L2/L3 mutants produced milder symptoms compared to wild type BCTV but the infectivity was reduced by 40% to 60%.”
Claim · model-written summary
L2/L3 mutant caused milder symptoms than wild type BCTV.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- L2/L3e2
- Interactor
- BCTVBeet curly top viruse2pathogen
- Outcome
- symptomse2
- Direction
- mildere2
- Comparator
- wild type BCTVe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana”
e2“R2, R3 and L2/L3 mutants produced milder symptoms compared to wild type BCTV but the infectivity was reduced by 40% to 60%.”
Claim · model-written summary
R2 mutant infectivity was reduced 40% to 60% relative to wild type BCTV.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- R2e2
- Interactor
- BCTVBeet curly top viruse2pathogen
- Outcome
- infectivitye2
- Direction
- reducede2
- Value
- 40% to 60%e2
- Comparator
- wild type BCTVe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana”
e2“R2, R3 and L2/L3 mutants produced milder symptoms compared to wild type BCTV but the infectivity was reduced by 40% to 60%.”
Claim · model-written summary
R3 mutant infectivity was reduced 40% to 60% relative to wild type BCTV.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- R3e2
- Interactor
- BCTVBeet curly top viruse2pathogen
- Outcome
- infectivitye2
- Direction
- reducede2
- Value
- 40% to 60%e2
- Comparator
- wild type BCTVe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana”
e2“R2, R3 and L2/L3 mutants produced milder symptoms compared to wild type BCTV but the infectivity was reduced by 40% to 60%.”
Claim · model-written summary
L2/L3 mutant infectivity was reduced 40% to 60% relative to wild type BCTV.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- L2/L3e2
- Interactor
- BCTVBeet curly top viruse2pathogen
- Outcome
- infectivitye2
- Direction
- reducede2
- Value
- 40% to 60%e2
- Comparator
- wild type BCTVe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana”
e2“R2, R3 and L2/L3 mutants produced milder symptoms compared to wild type BCTV but the infectivity was reduced by 40% to 60%.”
Claim · model-written summary
Only dsDNA accumulated in R2-infected organs.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Organ / stage
- organse3
- Outcome
- dsDNA was accumulatede3
- Qualifier
- R2-infectede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana”
e3“only dsDNA was accumulated on R2-infected organs.”
Claim · model-written summary
L4 disruption reduced infections.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Disruption of ORF L4e4
- Gene
- ORF L4e4
- Outcome
- infectionse4
- Direction
- reducede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana”
e4“Disruption of ORF L4 resulted in reduced infections, but the viral DNA was accumulated in infected organs from roots to shoot tips as much as wild type BCTV on Sei-O.”
Claim · model-written summary
L4 disruption allowed viral DNA accumulation from roots to shoot tips comparable with wild type BCTV on Sei-O.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- Sei-Oe4
- Treatment
- Disruption of ORF L4e4
- Outcome
- viral DNA was accumulatede4
- Comparator
- wild type BCTVe4
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana”
e4“Disruption of ORF L4 resulted in reduced infections, but the viral DNA was accumulated in infected organs from roots to shoot tips as much as wild type BCTV on Sei-O.”
Claim · model-written summary
Four mutants did not produce callus-like tissue on infected organs.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Organ / stage
- infected organse5
- Outcome
- did not produce callus-like tissuese5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana”
e5“4 mutants did not produce callus-like tissues on infected organs”
Claim · model-written summary
cycl mRNA accumulated in wild type BCTV symptomatic organs but not with the L4 mutant.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Organ / stage
- symptomatic organse6
- Treatment
- wild type BCTV infectionse6
- Gene
- cycle6
- Outcome
- cycl mRNA was accumulatede6
- Comparator
- L4 mutante6
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana”
e6“cycl mRNA was accumulated on symptomatic organs by wild type BCTV infections but not by L4 mutant.”
Claim · model-written summary
The authors conclude BCTV L4 ORF is essential for callus-like symptom formation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- BCTV L4 ORFe7
- Outcome
- callus-like formatione7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana”
e7“BCTV L4 ORF is essential for symptom developments, specially callus-like formation on infected organs.”
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