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“dry weight decrease”

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2000 ’10 ’20

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Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Double mutants had 35.2% lower total seed RFO content.

Taxon: soybeanGlycine maxt0
Outcome: total RFO contente0
Direction: decreasee0
Value: 35.2%e0
Qualifier: 64.7 mg/g dry weight to 41.95 mg/g dry weighte0

Verbatim

e0
“reduction in the total RFO content of soybean seed from 64.7 mg/g dry weight to 41.95 mg/g dry weight, a 35.2% decrease”
✓ located verbatim in the source · abstract, characters 875–994

t0
“Soybean Seeds”
✓ located verbatim in the source · title, characters 119–132

Title

CRISPR/Cas9-Mediated Knockout of Galactinol Synthase-Encoding Genes Reduces Raffinose Family Oligosaccharide Levels in Soybean Seeds

Huy Le; Nhung Hong Nguyen; Dong Thị Ta; et al. Frontiers in Plant Science 2020. doi:10.3389/fpls.2020.612942

Other claims from this paper (8)

Observation The construct induced deletions at both GOLS targets.
Observation Single and double mutant null segregants were identified without off-target induced mutations.
Observation Stachyose content decreased 35.4% in double mutants.
Observation Raffinose content increased 41.7% in double mutants.
Observation Verbascose content decreased slightly in mutant lines.
Observation Some mutant lines had increased protein and fat contents.
Observation Mutants showed no difference in seed weight, germination, development, or morphology.
Mechanism GmGOLS1A and GmGOLS1B contribute to the soybean oligosaccharide profile.

Observationobservedoriginal resultmeasured

Claim · model-written summary

The 042BM noeA sequence was 99% identical to the S. meliloti 1021 sequence.

Taxon: alfalfaMedicago sativae1
Gene: noeAt0
Outcome: identicale0
Value: 99%e0

Verbatim

e0
“It is identical to that of S. meliloti 1021 at 99% level, and similarity of their NoeA is 97%.”
✓ located verbatim in the source · abstract, characters 32–126

t0
“noeA from Sinorhizobium meliloti 042BM”
✓ located verbatim in the source · title, characters 46–84

e1
“Compared to 042BM, the noeA deletion mutant 042BMA-Km showed different degrees of increase in number of nodule, fresh weight of nodule and plant top dry weight on alfalfa cultivars of Putong Zihua, Baoding, Ningxia, Baifa and Aohan, but decrease on Milu.”
✓ located verbatim in the source · abstract, characters 401–655

Title

[Cloning, deletion and functional analysis of noeA from Sinorhizobium meliloti 042BM].

Bing-hai Du; Ju-quan Jiang; Xiao-hong Li; et al. PubMed 2005

Other claims from this paper (9)

Observation NoeA similarity between 042BM and S. meliloti 1021 was 97%.
Observation NoeA shared 32% similarity with a Mesorhizobium methyltransferase.
Observation NoeA residues 303-362 had 41% similarity to an E. coli methyltransferase region.
Intervention effect The noeA deletion mutant increased nodule number in five alfalfa cultivars.
Intervention effect The noeA deletion mutant increased nodule fresh weight in five alfalfa cultivars.
Intervention effect The noeA deletion mutant increased plant top dry weight in five alfalfa cultivars.
Intervention effect The noeA deletion mutant decreased the three measured traits on Milu alfalfa.
Intervention effect The mutant showed no significant change in nodulation of Huanghou and Zahua.
Mechanism The authors conclude that noeA contributes to cultivar-specific alfalfa nodulation.

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