Search claims · PlantClaims

Observationobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Closest loci had no significant diversity decrease beyond drift expectations.

Taxon: wheatTriticum aestivumt0
Gene: closest locie0
Outcome: gene diversitye0
Direction: no significant decreasee0

Verbatim

e0
“no significant decrease in gene diversity was found at the closest loci”
✓ located verbatim in the source · abstract, characters 1119–1190

t0
“wheat”
✓ located verbatim in the source · title, characters 64–69

Title

Soft selective sweep near a gene that increases plant height in wheat

A.‐L. RAQUIN; P. BRABANT; B. RHONÉ; et al. Molecular Ecology 2008. doi:10.1111/j.1365-294x.2007.03620.x

Other claims from this paper (6)

Observation Rht-B1 was under strong selection with s = 0.15.
Association Rht-B1 frequency variation accounted for 15% of trait evolution.
Observation Effective population size at Rht-B1 was 18 versus genome-wide 167.
Observation Hitch-hiking was expected to affect the two closest linked loci.
Observation Expected diversity reduction from hitch-hiking did not exceed genetic drift.
Mechanism Limited hitch-hiking was attributed to low linkage disequilibrium and high initial gene frequency.

Method / resourceobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

ePE6d precise small edits did not decrease significantly relative to ePE2.

Taxon: riceOryza sativat0
Outcome: precise edits by ePE6de0
Direction: decreasee0

Not reportedComparatorValue

Verbatim

t0
“rice”
✓ located verbatim in the source · title, characters 60–64

e0
“a significant decrease in precise edits by ePE6d was not observed throughout the targets”
✓ located verbatim in the source · abstract, characters 2001–2089

Title

Engineering PE6 prime editors to efficiently insert tags in rice

Rongfang Xu; Chong Ma; Jiaqi Sheng; et al. Plant Biotechnology Journal 2024. doi:10.1111/pbi.14456

Other claims from this paper (19)

Method / resource Both ePE6c and ePE6d precisely edited rice calli, at average rates of 7.08% and 19.82%.
Method / resource ePE6c had 1.83- to 10.12-fold lower small-edit efficiency than ePE2.
Method / resource ePE6d generated 11.62- to 580.98-fold more pegRNA scaffold byproducts than ePE2.
Method / resource ePE6c HA tag insertion efficiency was 6.87-fold lower than ePE2.
Method / resource ePE6d precise HA insertion reached 15.56%, 8.36-fold above ePE2, at ERF141-T.
Method / resource ePE6d precise HA insertion reached 14.10%, 2.72-fold above ePE2, at HLH109-T.
Method / resource ePE6d precise HA insertion reached 32.15%, 2.65-fold above ePE2, at TubA1-T.
Observation Incomplete insertions followed by flanking sequence replicates were the main ePE6d byproducts.
Method / resource ePE6d byproducts stayed comparable to ePE2 at HLH109-T and TubA1-T but rose slightly at ERF141-T.
Method / resource Precise c-MYC editing efficiency did not significantly differ between ePE2 and ePE6d.
Observation Predicted pegRNA extension structure for c-MYC was more disordered than for HA.
Method / resource ePE6d induced precise insertions in 20.83% to 70.83% of T0 lines versus 4.17% to 18.75% for ePE2.
Method / resource Transgenic byproducts occurred at comparable frequencies of 10.94% with ePE2 and 11.46% with ePE6d.
Method / resource ePE6d increased CBP insertion at ERF141-T, HLH109-T and TubA1-T by 2.26-, 5.65- and 7.56-fold, respectively.
Method / resource ePE6d improved 3×c-MYC insertion overall by 4.47-fold, reaching 33.84% at ERF141-T.
Method / resource ePE6d had higher edit to byproduct ratios than ePE2 in five of six edits.
Method / resource Tagged transgenic lines for CBP increased from 7.64% under ePE2 to 50% under ePE6d.
Method / resource Tagged transgenic lines for 3×c-MYC increased from 13.89% under ePE2 to 56.94% under ePE6d.
Method / resource ePE6d achieved maximal precise editing of 81.25% of T0 lines for CBP at TubA1-T and 66.67% for 3×c-MYC at ERF141-T; three other tags included successful 3×HA and 3×AVI insertion but no intact GB1 insertion.

Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Heat-induced expression did not significantly decrease rubisco protein relative to controls.

Taxon: wheatTriticum aestivumt0
Treatment: thise0
Outcome: rubisco protein contente0
Direction: did not induce a significant decreasee0
Comparator: controlse0

Verbatim

t0
“wheat”
✓ located verbatim in the source · title, characters 59–64

e0
“However this did not induce a significant decrease in rubisco protein content relative to controls.”
✓ located verbatim in the source · abstract, characters 999–1098

Title

Loss of decreased‐rubisco phenotype between generations of wheat transformed with antisense and sense rbcS

ROWAN A C MITCHELL; PRIYA A JOYCE; HONGLIN RONG; et al. Annals of Applied Biology 2004. doi:10.1111/j.1744-7348.2004.tb00377.x

Other claims from this paper (14)

Intervention effectobservedoriginal resultnot significant (as stated)measured

Claim · model-written summary

Extreme DAB concentrations did not significantly decrease mutation counts.

Taxon: WINTER WHEATTriticum aestivumt0
Treatment: extreme concentrations of this mutagenic agente0
Outcome: number of mutationse0
Direction: did not lead to a significant decreasee0

Not reportedComparator

Verbatim

e0
“Even extreme concentrations of this mutagenic agent did not lead to a significant decrease in the number of mutations”
✓ located verbatim in the source · abstract, characters 678–795

t0
“WINTER WHEAT”
✓ located verbatim in the source · title, characters 0–12

Title

WINTER WHEAT MUTATION VARIABILITY UNDER LOW-DAMAGE ABILITY MUTAGEN ACTION

Vladislav HORSHCHAR; Mykola NAZARENKO AgroLife Scientific Journal 2023. doi:10.17930/agl2023212

Other claims from this paper (3)

Intervention effect DAB produced valuable low growing, early and late ripening, high yielding, and tillering forms.
Association Mutagen characteristics affected the frequency of some mutation types.
Intervention effect Extreme DAB concentrations maximized the spectrum of possible changes.

Observationobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

No T2 line had a significant rubisco-content decrease relative to wild type.

Taxon: wheatTriticum aestivumt0
Material: these linese0
Outcome: rubisco contente0
Direction: no significant decreasee0
Comparator: wild‐typee0

Verbatim

t0
“wheat”
✓ located verbatim in the source · title, characters 59–64

e0
“There was no significant decrease in rubisco content relative to wild‐type in any of these lines (LSD of 8% for P = 0.05).”
✓ located verbatim in the source · abstract, characters 734–856

Title

Loss of decreased‐rubisco phenotype between generations of wheat transformed with antisense and sense rbcS

ROWAN A C MITCHELL; PRIYA A JOYCE; HONGLIN RONG; et al. Annals of Applied Biology 2004. doi:10.1111/j.1744-7348.2004.tb00377.x

Other claims from this paper (14)

Method / resource Wheat transformation efficiency was 1.2%.
Observation 31% of sense‐ rbcS transformed lines showed lower rubisco content than controls.
Observation 78% of antisense‐ rbcS transformed lines showed lower rubisco content than controls.
Observation Rubisco content decreases reached 60% in primary transformants.
Observation Only 5% of transgene-bearing T1 progeny had significantly decreased rubisco content.
Observation Five potential T2 homozygous wheat lines were identified.
Observation Antisense rbcS expression was low in two T2 lines.
Intervention effect Exposure to 37°C for 48 h increased antisense rbcS expression in T2 lines.
Intervention effect Heat-induced expression did not significantly decrease rubisco protein relative to controls.
Observation Two antisense lines had low copy number.
Observation Two antisense lines had 1–2 insertion events.
Observation Ubiquitin intron methylation increased in one T2 line relative to its primary transformant.
Mechanism The authors propose greater activity of the ubiquitin promoter due to greater stress in the T0 generation plants as a possible explanation for phenotype loss.
Mechanism The authors propose increased methylation of the transgene promoter region between generations as a possible explanation for phenotype loss.

Associationobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Roots of fro6-1 mutants did not show a significant decrease in ferric reductase activity compared with WT roots.

Taxon: Arabidopsis ThalianaArabidopsis thalianat0
Material: fro6-1e0
Organ / stage: rootse0
Outcome: ferric reductase activitye0
Direction: did not show a significant decreasee0
Comparator: the roots of WT plantse0

Verbatim

t0
“Arabidopsis Thaliana”
✓ located verbatim in the source · title, characters 61–81

e0
“fro6-1 mutant roots did not show a significant decrease in ferric reductase activity compared to the roots of WT plants”
✓ located verbatim in the source · abstract, characters 1550–1669

Title

Characterization of the Role of FRO6 in Metal Homeostasis in Arabidopsis Thaliana

Margo Ellen Maynes Scholar Commons (University of South Carolina) 2013

Other claims from this paper (5)

Intervention effectobservedoriginal resultpositivenot significant (as stated)measured

Claim · model-written summary

The double mutant showed a further numeric but nonsignificant disease decrease versus ΔtoxA.

Taxon: riceOryza sativae1
Treatment: ΔtoxA/prtA −e0
Outcome: disease developmente0
Direction: decreasee0
Comparator: ΔtoxA straine0

Verbatim

e0
“A double mutant deficient in both serine metalloprotease and toxoflavin (ΔtoxA/prtA − ) exhibited a further numeric but not statistically significant decrease of disease development compared with the ΔtoxA strain”
✓ located verbatim in the source · abstract, characters 797–1009

e1
“a major bacterial disease of rice”
✓ located verbatim in the source · abstract, characters 58–91

Title

The Virulence Function and Regulation of the Metalloprotease Gene prtA in the Plant-Pathogenic Bacterium Burkholderia glumae

Tiago Lelis; Jingyu Peng; Inderjit Barphagha; et al. Molecular Plant-Microbe Interactions 2019. doi:10.1094/mpmi-11-18-0312-r

Other claims from this paper (4)

Observation prtA null mutants lacked detectable extracellular protease activity.
Intervention effect Inoculation with prtA mutants significantly reduced rice panicle disease severity versus wild type.
Mechanism Extracellular protease activity depended on tofI/tofR and qsmR.
Mechanism Toxoflavin production depended on tofI/tofR and qsmR.

Observationobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

About 93% of divergent regions showed no significant decrease in heterozygosity, suggesting they are not approaching fixation.

Taxon: MaizeZea mayst0
Outcome: heterozygositye0
Direction: decreasee0
Value: 93%e0
Qualifier: not approaching fixatione0

Verbatim

t0
“Maize”
✓ located verbatim in the source · title, characters 50–55

e0
“Approximately 93% of the divergent regions do not demonstrate a significant decrease in heterozygosity, which suggests that they are not approaching fixation”
✓ located verbatim in the source · abstract, characters 813–970

Title

A Genome-Wide Scan for Evidence of Selection in a Maize Population Under Long-Term Artificial Selection for Ear Number

Timothy M Beissinger; Candice N Hirsch; Brieanne Vaillancourt; et al. Genetics 2014. doi:10.1534/genetics.113.160655

Other claims from this paper (5)

Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Applying 80 kPa tension after shoot removal prevented a significant decrease in PIP expression.

Taxon: Oryza sativa L.Oryza sativat0
Treatment: tension (80 kPa)e0
Outcome: PIP expressione0
Direction: prevented any significant decreasee0

Not reportedComparator

Verbatim

e0
“some tension (80 kPa) was applied. This application of tension prevented any significant decrease in PIP expression”
✓ located verbatim in the source · abstract, characters 1181–1296

t0
“Oryza sativa L.”
✓ located after whitespace/quote normalisation · title, characters 79–93

Title

Rapid changes in root hydraulic conductivity and aquaporin expression in rice (Oryza sativaL.) in response to shoot removal – xylem tension as a possible signal

Delong Meng; Marc Walsh; Wieland Fricke Annals of Botany 2016. doi:10.1093/aob/mcw150

Other claims from this paper (4)

Intervention effect Shoot removal rapidly and persistently decreased expression of three PIP1 and three PIP2 genes.
Intervention effect Expression of those aquaporins decreased by up to 85% within 60 minutes.
Observation The other three PIP2 genes had varied expression responses, including transient, delayed or no decrease.
Mechanism The authors infer that xylem tension has a role in the expression response to shoot removal.

Intervention effectobservedoriginal resultnot significant (as stated)measured

Claim · model-written summary

Inactivating the IPyA pathway caused a minor nonsignificant decrease in pathogenicity.

Taxon: Gypsophila paniculataGypsophila paniculatat0 e1
Treatment: Inactivation of the IPyA pathwaye0
Interactor: Erwinia herbicola pv. gypsophilaePantoea agglomerans pv. gypsophilaet0pathogen
Outcome: pathogenicitye0
Direction: decreasee0

Not reportedComparator

Verbatim

e0
“Inactivation of the IPyA pathway caused a minor, nonsignificant decrease in pathogenicity”
✓ located verbatim in the source · abstract, characters 582–671

t0
“Erwinia herbicola pv. gypsophilae”
✓ located verbatim in the source · title, characters 113–146

e1
“Erwinia herbicola pv. gypsophilae (Ehg), which induces galls on Gypsophila paniculata”
✓ located verbatim in the source · abstract, characters 0–85

Title

Differential Involvement of Indole-3-Acetic Acid Biosynthetic Pathways in Pathogenicity and Epiphytic Fitness of Erwinia herbicola pv. gypsophilae

Shulamit Manulis; Anat Haviv-Chesner; Maria T. Brandl; et al. Molecular Plant-Microbe Interactions 1998. doi:10.1094/mpmi.1998.11.7.634

Other claims from this paper (9)

Intervention effect Inactivating the IAM pathway reduced gypsophila gall size.
Intervention effect Inactivating cytokinin biosynthesis reduced gypsophila gall size.
Intervention effect Joint inactivation of both IAA pathways did not further reduce gall size.
Intervention effect Joint inactivation of both IAA pathways and cytokinin production did not further reduce gall size.
Intervention effect Inactivating IPyA reduced Ehg populations on bean 14-fold.
Intervention effect Inactivating IAM or cytokinin production did not affect epiphytic fitness.
Association iaaM-inaZ activity increased slightly in Ehg on bean and gypsophila leaves versus culture.
Observation iaaM-inaZ activity was highly induced in Ehg injected into gypsophila stems.
Association ipdC-inaZ showed moderate induction on leaves and in gypsophila stems versus culture.

“decrease in A”

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