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“decrease in dry weight”

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2020

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Taxon: soybean ✕

Taxon groups are the verbatim names found in the abstracts (case-insensitive), not normalised: maize, corn and Zea mays are separate groups here. The Organism view merges them through NCBI Taxonomy.

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Double mutants had 35.2% lower total seed RFO content.

Taxon: soybeanGlycine maxt0
Outcome: total RFO contente0
Direction: decreasee0
Value: 35.2%e0
Qualifier: 64.7 mg/g dry weight to 41.95 mg/g dry weighte0

Verbatim

e0
“reduction in the total RFO content of soybean seed from 64.7 mg/g dry weight to 41.95 mg/g dry weight, a 35.2% decrease”
✓ located verbatim in the source · abstract, characters 875–994

t0
“Soybean Seeds”
✓ located verbatim in the source · title, characters 119–132

Title

CRISPR/Cas9-Mediated Knockout of Galactinol Synthase-Encoding Genes Reduces Raffinose Family Oligosaccharide Levels in Soybean Seeds

Huy Le; Nhung Hong Nguyen; Dong Thị Ta; et al. Frontiers in Plant Science 2020. doi:10.3389/fpls.2020.612942

Other claims from this paper (8)

Observation The construct induced deletions at both GOLS targets.
Observation Single and double mutant null segregants were identified without off-target induced mutations.
Observation Stachyose content decreased 35.4% in double mutants.
Observation Raffinose content increased 41.7% in double mutants.
Observation Verbascose content decreased slightly in mutant lines.
Observation Some mutant lines had increased protein and fat contents.
Observation Mutants showed no difference in seed weight, germination, development, or morphology.
Mechanism GmGOLS1A and GmGOLS1B contribute to the soybean oligosaccharide profile.

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Double mutant seeds had 35.2% less total RFO content, falling from 64.7 to 41.95 mg/g dry weight.

Taxon: soybeanGlycine maxe0
Material: double mutant linese0
Organ / stage: seede0
Treatment: double mutant linese0
Outcome: total RFO contente0
Direction: reductione0
Value: 35.2%e0

Not reportedComparator

Verbatim

e0
“The seed carbohydrate analysis of double mutant lines showed a reduction in the total RFO content of soybean seed from 64.7 mg/g dry weight to 41.95 mg/g dry weight, a 35.2% decrease”
✓ located verbatim in the source · abstract, characters 804–986

Title

CRISPR/Cas9-mediated knockout of galactinol synthase-encoding genes reduces raffinose family oligosaccharide levels in soybean seeds

Phat Do; Ha Chu; Huy Le; et al. Proceedings of The 1st International Electronic Conference on Plant Science 2020. doi:10.3390/iecps2020-08852

Other claims from this paper (9)

Method / resource The CRISPR construct induced deletions at target sites of both genes.
Observation Some induced alleles passed to progeny and T2 null segregants lacked induced off-target mutations.
Intervention effect Stachyose content declined by 35.4% in double mutant soybean.
Intervention effect Raffinose content rose by 41.7% in double mutant soybean.
Intervention effect Verbascose content decreased slightly in mutant lines.
Intervention effect Some mutant lines had increased seed protein and fat contents.
Intervention effect Mutants showed no observed differences in seed germination, development or morphology.
Mechanism The authors infer that GmGOLS1A and GmGOLS1B contribute to soybean oligosaccharide profiles through RFO biosynthesis.
Recommendation The authors present the genes as targets for crop improvement.

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