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Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

E64 and Ac-Z-asp-DCB significantly affected the final tracheary element dimensions (length and area) of Zinnia elegans.

Taxon
Zinnia elegansZinnia eleganse1
Material
cv Envye1
Organ / stage
mesophyll cellse1
Treatment
Application of E64 (a general protease inhibitor) and Ac-Z-asp-DCB (a broad-range human caspase inhibitor)e0
Outcome
final TE dimensions (length and area)e0

Not reportedComparatorDirection

Verbatim

e0

“Application of E64 (a general protease inhibitor) and Ac-Z-asp-DCB (a broad-range human caspase inhibitor), both well established inhibitors of PCD in plants, resulted in a significant delay in the timing of cell death, the formation of secondary wall thickenings and significantly affected the final TE dimensions (length and area)”

✓ located verbatim in the source · abstract, characters 878–1210
e1

“Zinnia elegans cv Envy mesophyll cells”

✓ located verbatim in the source · abstract, characters 838–876

Title

Manipulation of programmed cell death in xylogenic Zinnia elegans suspension cell cultures influences the kinetics and dimensions of tracheary elements produced

Peter Twumasi; Ernst J. Woltering; W. van Ieperen; et al. Socio-Environmental Systems Modeling 2006

Other claims from this paper (3)
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

A-pao tobacco plants showed less programmed cell death than wild-type plants under salt stress.

Material
A-pao plantse1
Treatment
salt stresse2
Outcome
programmed cell deathe1
Direction
lesse1
Comparator
wild-type plantse1

Verbatim

e0

“we developed a series of tobacco (Nicotiana tabacum cv Xanthi) transgenic plants overexpressing or downregulating apoplastic polyamine oxidase (PAO; S-pao and A-pao plants, respectively) or downregulating S-adenosyl-l-methionine decarboxylase (samdc plants)”

✓ located verbatim in the source · abstract, characters 234–491
e1

“A-pao plants accumulated less H2O2 and exhibited less programmed cell death (PCD) than did wild-type plants, in contrast with S-pao and samdc downregulating plants”

✓ located verbatim in the source · abstract, characters 625–788
e2

“Upon salt stress, plants secreted spermidine (Spd) into the apoplast, where it was oxidized by the apoplastic PAO, generating H2O2”

✓ located verbatim in the source · abstract, characters 493–623

Title

Spermidine Exodus and Oxidation in the Apoplast Induced by Abiotic Stress Is Responsible for H2O2 Signatures That Direct Tolerance Responses in Tobacco

Panagiotis N. Moschou; Konstantinos A. Paschalidis; Ioannis D. Delis; et al. The Plant Cell 2008. doi:10.1105/tpc.108.059733

Other claims from this paper (9)
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

E64 and Ac-Z-asp-DCB significantly delayed cell death timing and secondary wall thickening formation in Zinnia elegans mesophyll cells.

Taxon
Zinnia elegansZinnia eleganse1
Material
cv Envye1
Organ / stage
mesophyll cellse1
Treatment
Application of E64 (a general protease inhibitor) and Ac-Z-asp-DCB (a broad-range human caspase inhibitor)e0
Outcome
timing of cell death, the formation of secondary wall thickeningse0
Direction
delaye0

Not reportedComparator

Verbatim

e0

“Application of E64 (a general protease inhibitor) and Ac-Z-asp-DCB (a broad-range human caspase inhibitor), both well established inhibitors of PCD in plants, resulted in a significant delay in the timing of cell death, the formation of secondary wall thickenings and significantly affected the final TE dimensions (length and area)”

✓ located verbatim in the source · abstract, characters 878–1210
e1

“Zinnia elegans cv Envy mesophyll cells”

✓ located verbatim in the source · abstract, characters 838–876

Title

Manipulation of programmed cell death in xylogenic Zinnia elegans suspension cell cultures influences the kinetics and dimensions of tracheary elements produced

Peter Twumasi; Ernst J. Woltering; W. van Ieperen; et al. Socio-Environmental Systems Modeling 2006

Other claims from this paper (3)
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

NADPH-dependent carbonyl reductase activity decreased after a lethal dose of H2O2 in BY-2 cells.

Taxon
tobacco Bright Yellow-2 (BY-2) cellse0
Treatment
lethal dose (1.0 mM) of H2O2e1
Gene
carbonyl reductasee3
Outcome
activity of a reduced nicotinamide adenine dinucleotide phosphate (NADPH)-dependent carbonyl reductasee3
Direction
decreasede3
Comparator
sublethal dose (0.5 mM) of H2O2e1

Verbatim

e0

“H2O2-induced programmed cell death (PCD) of tobacco Bright Yellow-2 (BY-2) cells”

✓ located verbatim in the source · abstract, characters 0–80
e1

“The following biochemical changes were observed in 10-min response to a lethal dose (1.0 mM) of H2O2, but they did not occur in a sublethal dose (0.5 mM) of H2O2. (1) The C3LP activity was increased twofold”

✓ located verbatim in the source · abstract, characters 312–518
e3

“The activity of a reduced nicotinamide adenine dinucleotide phosphate (NADPH)-dependent carbonyl reductase, scavenging HNE, and n-hexanal was decreased”

✓ located verbatim in the source · abstract, characters 651–802

Title

Inactivation of Carbonyl-Detoxifying Enzymes by H2O2 Is a Trigger to Increase Carbonyl Load for Initiating Programmed Cell Death in Plants

Md. Sanaullah Biswas; Ryota Terada; Jun’ichi Mano Antioxidants 2020. doi:10.3390/antiox9020141

Other claims from this paper (7)
Observationobservedoriginal resultmeasured

Claim · model-written summary

Identified proteins included the stated functional groups and proportions.

Taxon
Dimocarpus longanDimocarpus longane2
Outcome
proteins were unknown identity or functione0
Value
28.79%e0

Verbatim

e0

“28.79% of the proteins were unknown identity or function,30.30% of the proteins participated instress response and anti-oxidation,10.61% of the proteins were involved in energy and carbohydrate metabolism,10.61% of the proteins belonged to protein synthesis related,6.06% of the proteins were structural proteins involved in cytoskeleton remodeling,4.55% of the proteins were regulatory proteins,3.03%of the proteins wer…” Show full quote

✓ located verbatim in the source · abstract, characters 905–1370
e1

“Additionally,1.52% of the proteins were associated with signal transduction and PCD,1.52%of the proteins were related to amino acid metabolism,1.52% of the proteins were lipid metabolism-related protein,and 1.52% of the proteins attached themselves to vitamin metabolism”

✓ located verbatim in the source · abstract, characters 1371–1641
e2

“Dimocarpus longan Lour.cv.Honghezi”

✓ located verbatim in the source · abstract, characters 40–74

Title

Analysis of Protein Expression during Growth Processes of Friable Embryogenic Callus(FEC) of Longan

RA Li Xibei zhiwu xuebao 2015

Other claims from this paper (14)
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Intracellular RCS levels (4-hydroxy-(E)-hexenal and HNE) increased 1.2–1.5-fold after a lethal dose of H2O2 in BY-2 cells.

Taxon
tobacco Bright Yellow-2 (BY-2) cellse0
Treatment
lethal dose (1.0 mM) of H2O2e1
Outcome
intracellular levels of RCS, i.e., 4-hydroxy-(E)-hexenal and 4-hydroxy-(E)-nonenal (HNE)e2
Direction
increasede2
Value
1.2–1.5-folde2
Comparator
sublethal dose (0.5 mM) of H2O2e1

Verbatim

e0

“H2O2-induced programmed cell death (PCD) of tobacco Bright Yellow-2 (BY-2) cells”

✓ located verbatim in the source · abstract, characters 0–80
e1

“The following biochemical changes were observed in 10-min response to a lethal dose (1.0 mM) of H2O2, but they did not occur in a sublethal dose (0.5 mM) of H2O2. (1) The C3LP activity was increased twofold”

✓ located verbatim in the source · abstract, characters 312–518
e2

“The intracellular levels of RCS, i.e., 4-hydroxy-(E)-hexenal and 4-hydroxy-(E)-nonenal (HNE), were increased 1.2–1.5-fold”

✓ located verbatim in the source · abstract, characters 524–645

Title

Inactivation of Carbonyl-Detoxifying Enzymes by H2O2 Is a Trigger to Increase Carbonyl Load for Initiating Programmed Cell Death in Plants

Md. Sanaullah Biswas; Ryota Terada; Jun’ichi Mano Antioxidants 2020. doi:10.3390/antiox9020141

Other claims from this paper (7)

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