Search claims · PlantClaims

Observationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

ITS sequence analysis provides a new approach to identify Gastrodia elata resources.

Taxon: Gastrodia elataGastrodia elatae1
Outcome: new approach to identify the Gastrodia elata resourcese1

Verbatim

e1
“sequence analysis of ITS provides a new approach to identify the Gastrodia elata resources”
✓ located verbatim in the source · abstract, characters 1117–1207

Title

Identification of Different Types in Gastrodia elata Based on the Analysis of ITS Sequence

Dexin Wang Biotechnology(Faisalabad) 2010

Other claims from this paper (6)

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

ITS sequences distinctly separated isolates by anastomosis group.

Taxon: cornZea mays subsp. mayst0
Outcome: distinctly separatede0
Comparator: AG typese0

Verbatim

e0
“5.8S rDNA-ITS sequence analysis showed that the isolates could be distinctly separated based on their AG types”
✓ located verbatim in the source · abstract, characters 606–716

t0
“corn”
✓ located verbatim in the source · title, characters 30–34

Title

The anastomosis groups of the corn sheath blight pathogen Rhizoctonia spp. in northeastern China

Li Ju; Xia Hai‐Bo; YU Jin-feng Mycosystema 2011

Other claims from this paper (5)

Observation Corn sheath blight isolates belonged to eight listed Rhizoctonia groups.
Observation AG-1-IA was the largest anastomosis group at 38.46%.
Observation WAG-Z accounted for 26.92% of isolates.
Observation AG-5 accounted for 24.83% of isolates.
Observation AG4-HG-III was first isolated from maize in China.

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Three marker types were developed from the sequence alterations.

Taxon: TomatoSolanum lycopersicumt0
Outcome: a Kompetitive Allele Specific PCR marker, a sequencing marker, and an Insertion/Deletion marker were developede0

Not reportedComparatorValue

Verbatim

e0
“On the basis of these sequence alterations, a Kompetitive Allele Specific PCR marker, a sequencing marker, and an Insertion/Deletion marker were developed”
✓ located verbatim in the source · abstract, characters 1082–1236

t0
“Tomato”
✓ located verbatim in the source · title, characters 60–66

Title

Candidate Gene Identification and Transcriptome Analysis of Tomato male sterile-30 and Functional Marker Development for ms-30 and Its Alleles, ms-33, 7B-1, and stamenless-2

Kai Wei; Xin Li; Xue Cao; et al. International Journal of Molecular Sciences 2024. doi:10.3390/ijms25063331

Other claims from this paper (10)

Observation ms-30 and ms-33 exhibit twisted stamens, exposed stigmas and complete male sterility.
Observation ms-30 was fine mapped to 53.3 kb.
Observation ms-33 was fine mapped to 111.2 kb.
Observation TPI was identified as the likely gene for both loci and also for 7B-1 and sl-2.
Observation The four male sterile mutants were allelic.
Observation ms-30 had a TPI G-to-T transversion producing an S-to-I missense change.
Observation ms-33 had a transition causing alternative splicing and loss of 46 amino acids.
Observation 7B-1 and sl-2 had an approximately 4.8 kb retrotransposon insertion.
Observation Gene editing and allelism tests indicated that TPI is responsible for ms-30 and its alleles.
Observation Transcriptome and RT-PCR analyses found genes associated with stamen and carpel development.

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Direct nanopore sequencing detected Xylella fastidiosa and identified its subspecies only in plant samples with high bacterial levels.

Taxon: plant materialt0
Interactor: Xylella fastidiosaXylella fastidiosat0pathogen
Outcome: detection of Xfe0
Qualifier: only in samples with high bacterial levelse0

Not reportedComparatorValue

Verbatim

e0
“This allowed detection of Xf by direct DNA sequencing and identifying the subspecies only in samples with high bacterial levels.”
✓ located verbatim in the source · abstract, characters 725–853

t0
“Xylella fastidiosa subspecies and sequence types from naturally infected plant material”
✓ located verbatim in the source · title, characters 60–147

Title

Nanopore sequencing for the detection and identification of Xylella fastidiosa subspecies and sequence types from naturally infected plant material

Luigi Faino; Valeria Scala; Alessio Albanese; et al. Plant Pathology 2021. doi:10.1111/ppa.13416

Other claims from this paper (1)

Method / resource Amplicon nanopore sequencing identified Xylella fastidiosa at sequence type level in plant material with low bacterial infection within a few hours.

Observationauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

ITS sequence comparison and phylogenetic analysis provide useful genetic information for identifying the two herbal medicines and Brassica species.

Taxon: BrassicaBrassicae0
Gene: internal transcribed spacer (ITS) regionse3
Outcome: genetic information for the identificatione3

Verbatim

e0
“DNA barcodes of six Brassica species, B. juncea, B. napus, B. nigra, B. oleracea, B. rapa, and Sinapis alba, collected from various locations and markets”
✓ located verbatim in the source · abstract, characters 561–714

e3
“Nucleotide sequence comparison and phylogenetic analysis of internal transcribed spacer (ITS) regions based on the amphidiploid types and U's triangle provide useful genetic information for the identification of these two herbal medicines and Brassica species”
✓ located verbatim in the source · abstract, characters 827–1086

Title

Authentication of Brassicae Semen and Brassicae Campestris Semen by DNA Barcode Analysis

Pureum Noh; Sang Min Yeo; Wook Jin Kim; et al. Horticultural Science and Technology 2018. doi:10.12972/kjhst.20180086

Other claims from this paper (2)

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The SSR assay identified off-types and selfed seeds from F1 hybrid seed lots.

Taxon: hybrid riceOryza sativat0
Outcome: identify the off-types and the selfed seedse0
Comparator: F1 hybrid seedse0

Verbatim

e0
“could also effectively identify the off-types and the selfed seeds from its respective F1 hybrid seeds using these SSR primers”
✓ located verbatim in the source · abstract, characters 924–1050

t0
“hybrid rice”
✓ located verbatim in the source · title, characters 35–46

Title

Evaluation of genetic purity of F1 hybrid rice (Oryza sativa L.) with polymerase chain reaction-based simple sequence repeat markers

J. Shanthala; P.S. Aruna Bhavani; R. Siddaraju; et al. Current Advances in Agricultural Sciences(An International Journal) 2013

Other claims from this paper (5)

Method / resource RM 206 and RM 276 distinguished KRH-2 hybrids from parental lines.
Method / resource RM 204 and RM 228 distinguished DRRH-2 hybrids from parental lines.
Observation The SSR markers showed parental alleles in pure hybrids, confirming heterozygosity.
Method / resource SSR purity results were on par with field grow-out tests.
Recommendation The authors judged the SSR assays cost effective and suitable to replace conventional grow-out tests.

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Unmatched roots were putatively identified by ITS comparison with GenBank.

Outcome: putatively identifiede0
Comparator: GenBank databasee0

Not reportedTaxon

Verbatim

e0
“Roots which could not be matched to known types were putatively identified by comparison of the nuclear ribosomal ITS sequence to the GenBank database.”
✓ located verbatim in the source · abstract, characters 618–769

Title

Identification of roots from grass swards using PCR-RFLP and FFLP of the plastid trn L (UAA) intron

Karyn P Ridgway; Janette M Duck; J Peter W Young BMC Ecology 2003. doi:10.1186/1472-6785-3-8

Other claims from this paper (5)

Method / resource trnL product length and up to two restriction digests separated 14 grassland species.
Method / resource The RFLP key identified field root fragments at least to genus level.
Observation Ten taxa occurred among almost 600 root fragments.
Method / resource FFLP discriminated all taxa identified at the field site.
Method / resource A PCR-RFLP molecular database for common grassland species was developed.

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