Claim · model-written summary
ATP and glucose-6-phosphate decreased significantly in necrotic cells.
- Taxon
- soybean
Glycine maxt0 - Outcome
- The level of ATP and of glucose-6-phosphatee0
- Direction
- decreasede0
- Qualifier
- necrotic onese0
Verbatim
e0“The level of ATP and of glucose-6-phosphate remained constant in cells undergoing PCD, while it decreased significantly in the necrotic ones.”
t0“soybean suspension cell cultures”
Title
V. Casolo Journal of Experimental Botany 2005. doi:10.1093/jxb/eri093
Other claims from this paper (16)
- Intervention effect Hydrogen peroxide at 5 mM induced programmed cell death.
- Intervention effect Cells treated with 20 mM H2O2 became necrotic, with intermediate necrapoptotic hallmarks before that.
- Observation ATP and glucose-6-phosphate stayed constant during programmed cell death.
- Intervention effect Succinate-dependent oxygen consumption was slightly uncoupled in mitochondria from 5 mM H2O2-treated cells.
- Intervention effect Mitochondrial electrical potential difference weakly decreased after 5 mM H2O2 treatment.
- Intervention effect KCl caused greater partial dissipation of delta psi in treated mitochondria than in controls.
- Mechanism CsA induced delta psi formation in de-energized mitochondria through K+ efflux.
- Intervention effect CsA-induced K+ efflux was reduced in mitochondria from treated cells.
- Observation Mitochondria from 1 mM sodium nitroprusside-treated cells showed the same response pattern and apoptotic symptoms.
- Intervention effect Succinate-dependent oxygen consumption was completely uncoupled in necrotic cells treated with 20 mM H2O2.
- Intervention effect Delta psi generation was significantly inhibited in necrotic cells treated with 20 mM H2O2.
- Intervention effect CsA-dependent delta psi formation was prevented in necrotic cells treated with 20 mM H2O2.
- Intervention effect Mitochondrial swelling was partly prevented in apoptotic cells compared with control cells.
- Intervention effect Mitochondrial swelling was completely prevented in necrotic cells compared with control cells.
- Observation Moderate swelling accompanied slight outer-membrane rupture and cytochrome c release.
- Mechanism The authors implicate a K(+)ATP channel in H2O2- or NO-induced programmed cell death.