Claim · model-written summary
Analyses of classical mutants (gnom, monopteros, bodenlos, topless) showed that localised auxin biosynthesis, directional transport and downstream transcriptional responses are central to apical-basal axis establishment and organ initiation.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- gnom, monopteros (mp), bodenlos (bdl) and toplesse0
- Compound
- auxine0
- Outcome
- apical-basal axis establishment and organ initiatione0
Verbatim
t0“Arabidopsis”
e0“Genetic and molecular analyses of classical mutants including gnom, monopteros (mp), bodenlos (bdl) and topless revealed that localised auxin biosynthesis, directional transport and downstream transcriptional responses are central to apical-basal axis establishment and organ initiation”
Title
Imaging and genetic toolbox to study Arabidopsis embryogenesis
David Babić; Milan Župunski; Jiří Friml New Phytologist 2026. doi:10.1111/nph.71072
Other claims from this paper (5)
- Observation Following fertilisation the Arabidopsis zygote divides asymmetrically to generate apical and basal lineages, establishing the apical-basal axis that defines future shoot and root poles.
- Mechanism The main components of this regulation are polarly localised PIN auxin transporters and downstream modules involving MONOPTEROS and WUSCHEL-RELATED HOMEOBOX transcription factors.
- Observation Fluorescence-compatible clearing reagents and three-dimensional reconstructions now permit quantitative analyses of cell geometry, division orientation and cytoskeletal dynamics in Arabidopsis embryogenesis.
- Observation Live ovule imaging setups with confocal laser scanning and multiphoton microscopes enable real-time observation of Arabidopsis embryo development.
- Observation Laser-assisted cell ablation can be used to probe cell-to-cell communication and fate plasticity in Arabidopsis embryos.