Claim · model-written summary
The VdEGe1 knockout mutant of V. dahliae showed impaired colony expansion and hyphal growth.
- Taxon
- Verticillium dahliae
Verticillium dahliaet0 - Treatment
- ΔVdEGe1 knockoute1
- Gene
- VdEGe1e1
- Outcome
- colony expansion, hyphal growthe1
- Direction
- impairede1
Not reportedComparator
Verbatim
e1“The ΔVdEGe1 knockout mutant exhibited impaired colony expansion, hyphal growth, sporulation, and spore germination, accompanied by severely attenuated virulence”
t0“Verticillium dahliae”
Title
VdEGe1, a cell wall-degrading enzyme gene from Verticillium dahliae required for cotton pathogenesis
Yuanjing Li; Ruixiang Yuan; Yongtai Li; et al. BMC Microbiology 2026. doi:10.1186/s12866-026-04977-x
Other claims from this paper (11)
- Observation VdEGe1 of Verticillium dahliae was classified into glycoside hydrolase family 45 (GH45).
- Intervention effect Host-induced gene silencing of VdEGe1 significantly reduced Verticillium wilt symptoms, disease index and fungal biomass in infected cotton.
- Intervention effect The VdEGe1 knockout mutant of V. dahliae showed impaired sporulation and spore germination.
- Intervention effect The VdEGe1 knockout mutant of V. dahliae had severely attenuated virulence.
- Observation VdEGe1 was confirmed to be a secreted protein.
- Intervention effect VdEGe1 did not trigger or suppress cell death in Nicotiana benthamiana.
- Observation Secretome analysis identified 311 differentially expressed secretory proteins.
- Observation Of the differentially expressed secretory proteins, 156 were down-regulated.
- Intervention effect The VdEGe1 deletion mutant showed significant down-regulation of mitochondrial-related proteins and several carbohydrate-active enzymes.
- Intervention effect The VdEGe1 deletion mutant had decreased intracellular ATP levels and slower growth on media with various carbon sources.
- Mechanism The authors conclude that VdEGe1 deletion leads to mitochondrial dysfunction and impaired carbon utilization, inducing energy starvation in V. dahliae and compromising its growth, development and virulence.
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