Claim · model-written summary
Liquid medium with 0.2 M sucrose hastened regrowth signs to 3 days after thawing versus 24-25 days without preculture.
Olea europaeaVerbatim
e0“Preculture in liquid medium containing 0.2 M sucrose also had a significant influence on the initial response after cryopreservation, with first signs of regrowth resumption 3 days after thawing, compared to 24-25 days in control, non-precultured embryos.”
t0“olive somatic embryos”
Title
Fatiha Bradaï; Javier Almagro-Bastante; Carolina Sánchez-Romero Scientia Horticulturae 2023. doi:10.1016/j.scienta.2023.112385
Other claims from this paper (8)
- Association Inoculum density significantly affected regrowth rate after olive embryo cryopreservation.
- Association A significant effect of inoculum density on recovery percentage could not be inferred.
- Intervention effect Sucrose preculture significantly improved recovery after cryopreservation.
- Observation Liquid ECO medium with 0.2 M sucrose for 28 days gave the best cryopreservation results.
- Observation After that preculture, 90% of explants resumed embryogenesis at 12 weeks after thawing.
- Observation Surviving olive explants continued active growth with the usual growth pattern.
- Intervention effect Sucrose-enriched preculture greatly increased cell proliferation in somatic embryos.
- Mechanism The authors propose that meristematic and proembryogenic cell features promote survival and explain higher recovery.