Abstract
This study reports improvement of a protocol for cryopreservation of olive somatic embryos by using the droplet-vitrification method on aluminum foil strips. Two different approaches were considered in order to optimize cryopreservation: investigating the effect of somatic embryos growth conditions, specifically, the influence of the inoculum density of suspension cultures from which cryopreservation explants were collected, and studying the impact of preculture with different sucrose concentrations in both solid and liquid medium. The results obtained showed a significant effect of inoculum density on cryopreservation evaluated as regrowth rate, although a significant influence on recovery percentage could not be inferred. Sucrose preculture significantly improved recovery rates after cryopreservation. The best results were achieved with somatic embryos previously incubated in liquid ECO medium supplemented with 0.2 M sucrose for 28 days. After this treatment, 90% of explants resumed embryogenesis 12 weeks after thawing. Preculture in liquid medium containing 0.2 M sucrose also had a significant influence on the initial response after cryopreservation, with first signs of regrowth resumption 3 days after thawing, compared to 24-25 days in control, non-precultured embryos. Surviving explants continued actively growing, exhibiting the growth pattern normally observed in olive. As revealed by the histological analysis, cell proliferation greatly increased in somatic embryos precultured on sucrose-enriched media. Cellular features of meristematic and proembryogenic cells, mostly constituting the proliferative regions, make them more prone to survive cryopreservation, thus explaining higher recovery rates found after these treatments.
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Claims 9
Claim · model-written summary
Inoculum density significantly affected regrowth rate after olive embryo cryopreservation.
- Taxon
- olive
Olea europaeat0 - Treatment
- inoculum densitye0
- Outcome
- regrowth ratee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results obtained showed a significant effect of inoculum density on cryopreservation evaluated as regrowth rate, although a significant influence on recovery percentage could not be inferred.”
t0“olive somatic embryos”
Claim · model-written summary
A significant effect of inoculum density on recovery percentage could not be inferred.
- Taxon
- oliveOlea europaeat0
- Treatment
- inoculum densitye0
- Outcome
- recovery percentagee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results obtained showed a significant effect of inoculum density on cryopreservation evaluated as regrowth rate, although a significant influence on recovery percentage could not be inferred.”
t0“olive somatic embryos”
Claim · model-written summary
Sucrose preculture significantly improved recovery after cryopreservation.
- Taxon
- oliveOlea europaeat0
- Treatment
- Sucrose preculturee0
- Outcome
- recovery ratese0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sucrose preculture significantly improved recovery rates after cryopreservation.”
t0“olive somatic embryos”
Claim · model-written summary
Liquid ECO medium with 0.2 M sucrose for 28 days gave the best cryopreservation results.
- Taxon
- oliveOlea europaeat0
- Organ / stage
- somatic embryose0
- Treatment
- liquid ECO medium supplemented with 0.2 M sucrose for 28 dayse0
- Outcome
- best resultse0
- Direction
- beste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best results were achieved with somatic embryos previously incubated in liquid ECO medium supplemented with 0.2 M sucrose for 28 days.”
t0“olive somatic embryos”
Claim · model-written summary
After that preculture, 90% of explants resumed embryogenesis at 12 weeks after thawing.
- Taxon
- oliveOlea europaeat0
- Organ / stage
- explantse0
- Treatment
- this treatmente0
- Outcome
- resumed embryogenesise0
- Value
- 90%e0
- Qualifier
- 12 weeks after thawinge0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After this treatment, 90% of explants resumed embryogenesis 12 weeks after thawing.”
t0“olive somatic embryos”
Claim · model-written summary
Liquid medium with 0.2 M sucrose hastened regrowth signs to 3 days after thawing versus 24-25 days without preculture.
- Taxon
- oliveOlea europaeat0
- Treatment
- Preculture in liquid medium containing 0.2 M sucrosee0
- Outcome
- first signs of regrowth resumptione0
- Direction
- lowerinferrede0
- Value
- 3 dayse0
- Qualifier
- 24-25 dayse0
- Comparator
- control, non-precultured embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Preculture in liquid medium containing 0.2 M sucrose also had a significant influence on the initial response after cryopreservation, with first signs of regrowth resumption 3 days after thawing, compared to 24-25 days in control, non-precultured embryos.”
t0“olive somatic embryos”
Claim · model-written summary
Surviving olive explants continued active growth with the usual growth pattern.
- Taxon
- oliveOlea europaeae0
- Organ / stage
- explantse0
- Outcome
- continued actively growing, exhibiting the growth pattern normally observed in olivee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Surviving explants continued actively growing, exhibiting the growth pattern normally observed in olive.”
Claim · model-written summary
Sucrose-enriched preculture greatly increased cell proliferation in somatic embryos.
- Taxon
- oliveOlea europaeat0
- Organ / stage
- somatic embryose0
- Treatment
- sucrose-enriched mediae0
- Outcome
- cell proliferatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“As revealed by the histological analysis, cell proliferation greatly increased in somatic embryos precultured on sucrose-enriched media.”
t0“olive somatic embryos”
Claim · model-written summary
The authors propose that meristematic and proembryogenic cell features promote survival and explain higher recovery.
- Taxon
- oliveOlea europaeat0
- Organ / stage
- meristematic and proembryogenic cellse0
- Outcome
- higher recovery ratese0
- Direction
- highere0
- Qualifier
- Cellular features of meristematic and proembryogenic cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cellular features of meristematic and proembryogenic cells, mostly constituting the proliferative regions, make them more prone to survive cryopreservation, thus explaining higher recovery rates found after these treatments.”
t0“olive somatic embryos”
The same questions in other papers
sucrose · growth · Olea europaea (common olive)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
in vitro · growth · Olea europaea (common olive)Positive and negative results
16 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
15 papers more in this group
See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.