Abstract
Olive (Olea europea L.) somatic embryos were successfully cryopreserved using encapsulation-dehydration and encapsulation-vitrification. In the encapsulation-dehydration procedure, a maximum of 48% embryo survival was obtained when bead moisture content was decreased to 21.1% after 4 h dehydration. Preculture of embryos for 4 d in medium containing 0.75 to 1.25 M sucrose produced higher (40 to 34 %, respectively) regrowth after cryopreservation using encapsulation-dehydration procedure. Dehydration of beads for 3 h in PVS2 ensured higher survival (64%) of encapsulated-vitrified and cryopreserved (EVN) somatic embryos. Thermal treatment of embryogenic callus for 1 d at 30 degree C was very effective to increase survival of encapsulated-dehydrated and cryopreserved (EDN) (58%) and EVN (68%) embryos. Plantlets produced from control and cryopreserved embryos were phenotypically similar.
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Claims 8
Claim · model-written summary
Olive somatic embryos were successfully cryopreserved by encapsulation-dehydration.
- Taxon
- Olive
Olea europaeae0 - Treatment
- encapsulation-dehydratione0
- Outcome
- somatic embryos were successfully cryopreservede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Olive (Olea europea L.) somatic embryos were successfully cryopreserved using encapsulation-dehydration and encapsulation-vitrification.”
Claim · model-written summary
Olive somatic embryos were successfully cryopreserved by encapsulation-vitrification.
- Taxon
- OliveOlea europaeae0
- Treatment
- encapsulation-vitrificatione0
- Outcome
- somatic embryos were successfully cryopreservede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Olive (Olea europea L.) somatic embryos were successfully cryopreserved using encapsulation-dehydration and encapsulation-vitrification.”
Claim · model-written summary
Encapsulation-dehydration produced a maximum olive embryo survival of 48% at 21.1% bead moisture after four hours.
- Taxon
- OliveOlea europaeat0
- Treatment
- 4 h dehydratione0
- Outcome
- embryo survivale0
- Direction
- maximume0
- Value
- 48%e0
- Qualifier
- 21.1%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a maximum of 48% embryo survival was obtained when bead moisture content was decreased to 21.1% after 4 h dehydration.”
t0“olive”
Claim · model-written summary
Preculture in 0.75 M sucrose for four days produced 40% olive embryo regrowth after cryopreservation.
- Taxon
- OliveOlea europaeat0
- Treatment
- 0.75 to 1.25 M sucrosee0
- Outcome
- regrowthe0
- Direction
- highere0
- Value
- 40 to 34 %e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Preculture of embryos for 4 d in medium containing 0.75 to 1.25 M sucrose produced higher (40 to 34 %, respectively) regrowth after cryopreservation using encapsulation-dehydration procedure.”
t0“olive”
Claim · model-written summary
Three hours of PVS2 dehydration yielded 64% survival of encapsulated, vitrified and cryopreserved olive embryos.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Dehydration of beads for 3 h in PVS2 ensured higher survival (64%) of encapsulated-vitrified and cryopreserved (EVN) somatic embryos.”
t0“olive”
Claim · model-written summary
One day of thermal treatment at 30 degrees C raised survival of encapsulated-dehydrated olive embryos to 58%.
- Taxon
- OliveOlea europaeat0
- Treatment
- Thermal treatment of embryogenic callus for 1 d at 30 degree Ce0
- Outcome
- survival of encapsulated-dehydrated and cryopreserved (EDN)e0
- Direction
- increasee0
- Value
- 58%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Thermal treatment of embryogenic callus for 1 d at 30 degree C was very effective to increase survival of encapsulated-dehydrated and cryopreserved (EDN) (58%) and EVN (68%) embryos.”
t0“olive”
Claim · model-written summary
One day of thermal treatment at 30 degrees C raised survival of encapsulated-vitrified olive embryos to 68%.
- Taxon
- OliveOlea europaeat0
- Treatment
- Thermal treatment of embryogenic callus for 1 d at 30 degree Ce0
- Outcome
- survivale0
- Direction
- increasee0
- Value
- 68%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Thermal treatment of embryogenic callus for 1 d at 30 degree C was very effective to increase survival of encapsulated-dehydrated and cryopreserved (EDN) (58%) and EVN (68%) embryos.”
t0“olive”
Claim · model-written summary
Plantlets from cryopreserved and control olive embryos were phenotypically similar.
- Taxon
- OliveOlea europaeat0
- Outcome
- phenotypically similare0
- Comparator
- control and cryopreserved embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plantlets produced from control and cryopreserved embryos were phenotypically similar.”
t0“olive”
The same questions in other papers
sucrose · growth · Olea europaea (common olive)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
drought · survival · Olea europaea (common olive)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.