Claim · model-written summary
Live ovule imaging setups with confocal laser scanning and multiphoton microscopes enable real-time observation of Arabidopsis embryo development.
- Taxon
- Arabidopsis
Arabidopsist0 - Outcome
- real-time observation of embryo developmente0
Verbatim
t0“Arabidopsis”
e0“Live ovule imaging setups with confocal laser scanning and multiphoton microscopes enable real-time observation of embryo development”
Title
Imaging and genetic toolbox to study Arabidopsis embryogenesis
David Babić; Milan Župunski; Jiří Friml New Phytologist 2026. doi:10.1111/nph.71072
Other claims from this paper (5)
- Observation Following fertilisation the Arabidopsis zygote divides asymmetrically to generate apical and basal lineages, establishing the apical-basal axis that defines future shoot and root poles.
- Mechanism Analyses of classical mutants (gnom, monopteros, bodenlos, topless) showed that localised auxin biosynthesis, directional transport and downstream transcriptional responses are central to apical-basal axis establishment and organ initiation.
- Mechanism The main components of this regulation are polarly localised PIN auxin transporters and downstream modules involving MONOPTEROS and WUSCHEL-RELATED HOMEOBOX transcription factors.
- Observation Fluorescence-compatible clearing reagents and three-dimensional reconstructions now permit quantitative analyses of cell geometry, division orientation and cytoskeletal dynamics in Arabidopsis embryogenesis.
- Observation Laser-assisted cell ablation can be used to probe cell-to-cell communication and fate plasticity in Arabidopsis embryos.