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“simultaneously detect the two pathogens”

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2010 ’20

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Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

The duplex PCR assay detects the two pathogens simultaneously at a limit of about 0.05 ng/µL.

Taxon: cottonGossypium hirsutumt0
Outcome: simultaneously detect the two pathogense3
Value: 0.05 ng/µLe3

Verbatim

t0
“from cotton in Australia”
✓ located verbatim in the source · title, characters 121–145

e3
“a duplex PCR assay was developed to simultaneously detect the two pathogens at a limit of an approximately 0.05 ng/µL”
✓ located verbatim in the source · abstract, characters 708–825

Title

Duplex PCR for the detection of the morphologically indistinguishable Berkeleyomyces rouxiae and Berkeleyomyces basicola from cotton in Australia

Chi P. T. Nguyen; Andrew Chen; Duy P. Le Australasian Plant Pathology 2026. doi:10.1007/s13313-026-01096-z

Other claims from this paper (5)

Method / resource The duplex PCR assay identifies B. rouxiae and B. basicola simultaneously without sequencing.
Method / resource A new primer specifically amplifies a 159 bp amplicon of B. basicola.
Method / resource The duplex PCR is sensitive enough to detect B. rouxiae from crude extracts prepared with a 5-min microwave-based DNA extraction.
Method / resource Nested PCR combined with the duplex PCR successfully detects the BRR pathogen in 15–30 mg of diseased cotton tissue.
Recommendation The duplex PCR is a valuable diagnostic tool for monitoring BRR pathogens in cotton in Australia.

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The assay detected both pathogens simultaneously in two rice seedlots.

Taxon: RiceOryza sativat0
Interactor: Xanthomonas oryzae pv. oryzaeXanthomonas oryzae pv. oryzaet0pathogen
Second interactor: X. oryzae pv. oryzicolaXanthomonas oryzae pv. oryzicolat0pathogen
Outcome: detected both pathogens simultaneouslye0
Value: two seedlotse0

Verbatim

e0
“We also detected both pathogens simultaneously in two seedlots, which successfully proved that PLPs (P-Xoo and P-Xoc) combined with reverse dotblot hybridization can be used to simultaneously detect multiple pathogens in naturally infested commercial rice seedlots.”
✓ located verbatim in the source · abstract, characters 1191–1456

t0
“Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola in Rice Seed”
✓ located verbatim in the source · title, characters 26–96

Title

Simultaneous Detection of Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola in Rice Seed Using a Padlock Probe-Based Assay

Yanli Tian; Yuqiang Zhao; Rui Xu; et al. Phytopathology 2014. doi:10.1094/phyto-10-13-0274-r

Other claims from this paper (8)

Method / resource The assay detected X. oryzae pv. oryzae genomic DNA at 1 pg.
Method / resource The assay detected X. oryzae pv. oryzae in seedlots with 2% artificially infested seed.
Method / resource The assay detected X. oryzae pv. oryzicola genomic DNA at 1 pg.
Method / resource The assay detected X. oryzae pv. oryzicola in seedlots with 0.2% artificially infested seed.
Method / resource PLP detected X. oryzae pv. oryzae in 39.3% of naturally infested commercial seedlots.
Method / resource PLP detected X. oryzae pv. oryzicola in 21.3% of naturally infested commercial seedlots.
Method / resource Conventional PCR detected X. oryzae pv. oryzae in 9.1% of the same seedlots.
Method / resource Conventional PCR detected X. oryzae pv. oryzicola in 8.5% of the same seedlots.

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“simultaneously detect the two pathogens”

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