Claim · model-written summary
The RPA-CRISPR/Cas12a assay achieved a detection limit of 1 copy/μL per reaction for Alternaria alternata, surpassing the sensitivity of conventional PCR/qPCR.
- Taxon
- pear
Pyrus communist0 - Interactor
- Alternaria alternata
Alternaria alternatat0pathogen - Outcome
- detection limite1
- Direction
- surpassinge1
- Value
- 1 copy/μLe1
- Comparator
- conventional PCR/qPCR methodse1
Verbatim
t0“Alternaria alternata causing pear black spot”
e1“achieved a detection limit of 1 copy/μL per reaction, surpassing the sensitivity of conventional PCR/qPCR methods”
Title
Liqing Zhang; Wei Jiang; Zhiwei Song; et al. LWT 2026. doi:10.1016/j.lwt.2026.119301
Other claims from this paper (3)
- Method / resource The optimized assay showed high specificity for A. alternata.
- Method / resource Field validation with 54 pear leaf and fruit samples showed complete concordance with PCR.
- Method / resource The entire workflow, from simplified DNA extraction to visual interpretation, can be completed within 40 min.